果汁中维生素检测方案

No.TIF-06302 KEM Application Note Kyoto Electronics Oxidation-Reduction titration No.NZ22-06278 Vitamin C in soft drink -Abstract- According to JAS for determination of vitamin C， indophenol is titrated with testsample. This method requires complex process by exchanging titration solution foreach sample. By our method， the sample is titrated with indophenol of which factoris predetermined so that the concentration can be easily obtained. -Equipment- Main unit ： Automatic potentiometric titrator AT-510 Detector ： Standard preamplifier STD-510 Combination Pt electrode C-272 #429-0012 -Reagent- Titration solution ： indophenol solution 一Method- (1)To 200mL beaker， About 3g sample was added. (2) Added pure water to make it total 100mL， and added 5 drops acetic acid. (3)Titrated with indophenol solution to determine concentration. -Calculation- Concentration(%)：(EP1-BL1)xC1xK1/SIZE EP1：volume(mL) BL1： blank (mL)=0 SIZE： sample size (mL)C1： concentration conversion (mg/mL)=0.2712K1： unit conversion coefficient=0.1 -Ambient condition- Room temperature ：22.5℃ Humidity ： 47%Weather ： cloudy -Literature- JAS Japan Agricultural Standard (Bulletin No.1226 September 29 1990) Tel：021-66581985，63172460 Fax： 021-66581986 周峰13916388882 zhou-feng1027@163.com -Results- Run Size (g) Vol. (mL) Conc.(%) 1 st2nd3rd 3.01083.04853.0213 6.58206.66836.6220 0.05930.05930.0594 Statistics MeanSD RSD 0.0594%0.0001% 0.1354% -Comment- Http：//www.shxf17.com Yttp：//www.shxfcom上海旭风科学仪器有限公司 上海市闸北区荣路 Y海旭风科学仪器有限公司 上海市闸北区荣路 el： Fax： 周峰 zhou-fengcom