• Easy transfer of an EP monograph HPLC method from an Agilent® 1260 Infinity LC system to a Thermo Scientific™ Vanquish™ Core HPLC system is demonstrated.• Enhanced hardware features of the Vanquish Core HPLC system enable flexible adjustments of the overall system gradient delay volume, which facilitates fine-tuning during the transfer.• Equivalent chromatographic results are obtained with the originating and receiving instrument.