碳13呼气检测器中性能研究检测方案

SerCon Ltd Tel: +44(0)1270 580008 Fax: +44(0)1270 252310 www.sercongroup.com (​http:​/​​/​www.sercongroup.com​) e-mail: sercon@btinternet.com ABCA Automated Breath 13C Analyser Introduction 介绍 Carbon –13 breath analysis is a common, reliable and non-invasive test for metabolic and digestive disorders. The ABCA can be used to routinely measure 13C enrichment in breath in nutritional, medicinal or veterinary research. 碳13呼气检测是普通的、可靠的、非侵入性的用于消化和代谢紊乱的测试。ABCA可以测量呼气中的13C分度，用于营养、医学和兽医领域的研究。 Method 方法 The ABCA is a continuous flow IRMS which is easy to operate and maintain and provides high quality performance on a budget. Breath samples are taken in 10ml septum sealed containers before and after patients are fed a 13C labelled substrate. A needle probe flushes only 2ml of the sample onto a GC, allowing for repeat analysis if required. The CO2 is purified ready for measurement on the stable isotope analyser. The 190 position auto-sampler is fully software integrated with automated reference gas calibration and bar code reader to accurately track sample tubes. An ABCA – NT system was investigated for accuracy and precision of 13C breath analysis. ABCA是连续流的稳定同位素比质谱仪，它操作和维护简便，高质量的输出指标，运行费用低。病人服下13C标记物前后的呼气样本放于10ml的隔膜密封的小瓶中。取样针只抽取2ml的样品到GC即可，如需要可以重复提取分析。190位的自动进样器完全软件控制，结合自动的参考气校准和条形码读取器用于精确地追踪样品管。 Accuracy :- Pre and post dose breath samples were measured by ABCA and on dual-inlet IRMS. The results show excellent agreement between the two techniques over a range of 0 to 88‰ 13C vs. PDB (Fig 1). 精度：前后分别使用ABCA和双路进样系统分析呼气样品结果是有规律的。这样的结果表示了这两种方法在13C是0 – 88‰的范围具有极好的一致性。 Fig 1: Comparison of breath samples on an ABCA and a dual-inlet IRMS system. Results are expressed as 13C excess (‰); the difference between pre and post-dose samples. ABCA和双路进样系统呼气样品对照。 Precision 精度（重复性）： The external precision of 13C analysis by ABCA was tested by multiple measurements of three different gases and a breath sample. The gases were measured against a reference gas (-32.66‰ 13C vs PDB). The external precision (1 SD) for each samples was less than 0.1‰ (Table 1). 小于 0.1‰ Applications 应用 13C Urea – Helicobacter pylori : the cause of gastric ulcers and possibly cancers. 13C 尿素检测—幽门杆菌：导致胃溃疡和可能引发胃癌。 13C Xylose – Bacterial Overgrowth : nutrient malabsorption and malnutrition. 13C木糖检测—细菌的繁殖过度：营养吸收障碍和营养失调研究。 13C Triglyceride 13C Trioctanoin – Fat Malabsorption and Pancreatic Function. 13C甘油三酸酯/13C三辛酸甘油酯检测—脂肪吸收障碍和胰腺功能研究。 13C Aminopyrine Erythromycin / 13C Methacetin – Liver Function & Diseases 13C氨基比林 红霉素/13C 麦撒西丁检测—肝功能和疾病研究。 13C Acetate/ 13C Octanoate - Gastric Emptying 13C醋酸盐/ 13C辛酸盐—胃排空研究。 Table 1 Analytical precision for 13C measurements of reference gases and breath. Values are 13C (‰) vs. PDB. Sample Gas 1 Gas2 Gas3 Breath 1 -32.27 -26.58 -8.11 -25.73 2 -32.32 -26.61 -8.01 -25.72 3 -32.25 -26.54 -7.96 -25.75 4 -32.26 -26.63 -7.91 -25.81 5 -32.22 -26.55 -7.93 -25.71 Mean -32.26 -26.58 -7.98 -25.74 SD 0.03 0.04 0.08 0.04 Linearity 线性 The linearity was tested by measuring a reference gas over a range of samples sizes (33.5 to 2.3 µmol CO2). Each sample was calibrated against 33.5 µmol CO2 of the reference gas. (Fig 2). 线性测试是测量全样品大小(33.5 to 2.3 µmol CO2)的参考气。每个样品用33.5 µmol CO2 的样品气校准。 Fig 2. Linearity of measured 13C/12C ratio with sample size. Conclusions 结论 Automated breath 13C analysis is accurate, precise and applicable to the measurement of 13C enrichment in breath samples. The speed and simplicity of 13C breath tests with the ABCA permits rapid turnaround of patient samples and provides the clinician with the exact results needed for accurate diagnosis 自动13C呼气检测是精确的、准确的和适于呼气样品中13C丰度 的测量。 References Tytgat, G.N.J et cal (1990) Working Party Report of the World Congress of Gastroenterology, 36-45. Graham, D.Y. et al (1987) Lancet, 1174-1177. Logan, R et al (1990) Rev. Esp. Enf. Digest., 78 (Supl. 1), 14. Choi, M.G. et al (1998) Am. J. Gastroenterol. 93. 92-8. Dellert, S.F. et al (1997) J Pediatr. Gastroenterol. Nutr., 25, 153-8. Mion, F. et al. (1995). Hepato-Gastroenterology, 42, 931-8. The Stable Isotope Company Company Registration Number in England and Wales 4290072 VAT Registration No. GB 771 1100 74 Registered Office: 5 Dunnillow Field, Stapeley, Nantwich, Cheshire. CW5 7GX