活细胞培养中CO2浓度，温度，O2浓度检测方案(活体成像系统)

mBio，2014，10.1128/mBio.01850-14 ibidi Heating and Incubation System mentioned in publications： Cutting off the power： inhibition of leukemia cell growth by pausing basal ATP release andP2X receptor signaling? C. Ledderose， T. Woehrle， S. Ledderose， K. Strasser， R. Seist， Y. Bao， J. Zhang and W. JungerPurinergic Signalling， 2016， 10.1007/s11302-016-9510-y read abstract Accelerated actin filament polymerization from microtubule plus ends J. Henty-Ridilla， A. Rankova， J. Eskin， K. Kenny and B. Goode Science， 2016， 10.1126/science.aaf1709 read abstract Time-Resolved Study of Nanoparticle Induced Apoptosis Using Microfabricated Single CellArrays P. Rottgermann， K. Dawson and J. Radler Microarrays， 2016， 10.3390/microarrays5020008 read abstract Kinetics of sickle cell biorheology and implications for painful vasoocclusive crisis E. Du， M. Diez-Silva， G. Kato， M. Dao and S. Suresh Proceedings of the National Academy of Sciences， 2015， 10.1073/pnas.1424111112read abstract Wavelet transform analysis of chromatin texture changes during heat shock G. Herbomel， A. Grichine， A. Fertin， A. Delon， H. VOURC， C. Souchier and Y. UssonJournal of microscopy，2015， 10.1111/jmi.12363 read abstract Stability analysis of chemically modified mRNA using micropattern-based single-cellarrays M. Ferizi，C. Leonhardt，C. Meggle， M. Aneja， C. Rudolph， C. Plank and J. Radler Lab on a Chip， 2015， 10.1039/C5LC00749F read abstract Regulatory T Cell-Derived Adenosine Induces Dendritic Cell Migration through the Epac-Rap1 Pathway S. Ring，A. Pushkarevskaya，H. Schild， H. Probst， V. Jendrossek， F. Wirsdorfer， C. Ledent， S.Robson， A. Enk and K. Mahnke The Journal of Immunology，2015， 10.4049/jimmunol.1401434 read abstract Characterization of a Replication-Incompetent Pseudorabies Virus Mutant Lacking theSole Immediate Early Gene IE180 B. Wu，E. Engel and L. Enquist read abstract Expression of the oestrogen receptor GPER by testicular peritubular cells is linked to sexual maturation and male fertility F. Sandner， H. Welter， J. U. Schwarzer， F. M. Kohn， H. F. Urbanski and A. MayerhoferAndrology， 2014， 10.1111/j.2047-2927.2014.00243.x read abstract Cell surface engineering using glycosylphosphatidylinositol anchored tissue inhibitor ofmatrix metalloproteinase-1 stimulates cutaneous wound healing R. Djafarzadeh， C. Conrad， S. Notohamiprodjo，S. Hipp， H. Niess， C. Bruns and P. Nelson Wound Repair and Regeneration， 2014， 10.1111/wrr.12132 read abstract Enhanced quantitative phase imaging in self-interference digital holographic microscopyusing an electrically focus tunable lens R. Schubert， A. Vollmer， S. Ketelhut and B. Kemper Biomedical Optics Express， 2014， 10.1364/BOE.5.004213 read abstract Arraying Cell Cultures Using PEG-DMA Micromolding in Standard Culture Dishes A.-K. Marel， S.Rappl， A. Piera Alberola and J. O. Radler Macromolecular Bioscience， 2013， 10.1002/mabi.201200400 read abstract Dye selection for live cell imaging of intact siRNA M.Hirsch， D. Strand and M. Helm Biological Chemistry， 2012， read abstract CtIP Mutations Cause Seckel and Jawad Syndromes P. Qvist， P. Huertas， S. Jimeno， M. Nyegaard， M. J. Hassan， S. P. Jackson and A. D. BorglumPLoS Genet， 2011，10.1371/journal.pgen.1002310 read abstract A new assay for nerve fiber repulsion A. Fassold and R. H. Straub Annals of the New York Academy of Sciences， 2010， 10.1111/j.1749-6632.2009.05295.xread abstract Autophagy supports Candida glabrata survival during phagocytosis A. Roetzer， N. Gratz， P. Kovarik and C. Schuller Cellular Microbiology， 2010， 10.1111/j.1462-5822.2009.01391.x read abstract Contrast-enhanced digital holographic imaging of cellular structures by manipulating theintracellular refractive index C. E. Rommel， C. Dierker，L. Schmidt， S. Przibilla， G. von Bally， B. Kemper and J. Schnekenburger Journal of Biomedical Optics， 2010， 10.1117/1.3449567 read abstract TCR-Induced Activation of Ras Proceeds at the Plasma Membrane and RequiresPalmitoylation of N-Ras I. Rubio， S. Grund， S.-P. Song， C. Biskup， S. Bandemer， M. Fricke， M. Forster， A. Graziani， U. Wittig and S. Kliche The Journal of Immunology， 2010， doi：10.4049/jimmunol.1000334 read abstract The carboxyl tail of Cx43 augments p38 mediated cell migration in a gap junction-independent manner J. Behrens， P. Kameritsch， S. Wallner， U. Pohl and K. Pogoda European Journal of Cell Biology， 2010， 10.1016/j.ejcb.2010.06.003 read abstract Regulation of DNA-damage responses and cell-cycle progression by the chromatinremodelling factor CHD4 S. E. Polo， A. Kaidi， L. Baskcomb， Y. Galanty and S. P. Jackson The EMBO Journal，2010， 10.1038/emboj.2010.188 read abstract Regulation of adhesion behavior of murine macrophage using supported lipid membranesdisplaying tunable mannose domains T. Kaindl， J. Oelke， A. Pasc， S.Kaufmann，O.V. Konovalov，S. S. Funari， U. Engel， A. Wixforthand M.Tanaka Journal of Physics： Condensed Matter， 2010， 10.1088/0953-8984/22/28/285102 read abstract VE-PTP controls blood vessel development by balancing Tie-2 activity M. Winderlich， L. Keller， G. Cagna， A. Broermann，O. Kamenyeva， F. Kiefer， U.Deutsch， A. F. Nottebaum and D. Vestweber J. Cell Biol.， 2009， 10.1083/jcb.200811159 read abstract Mammalian SUMO E3-ligases PIAS1 and PIAS4 promote responses to DNA double-strandbreaks Y. Galanty， R. Belotserkovskaya， J. Coates， S. Polo， K. M. Miller and S. P. Jackson Nature， 2009， 10.1038/nature08657 read abstract Inhibition of "self" engulfment through deactivation of myosin-ll at the phagocytic synapsebetween human cells R. K. Tsai and D. E. Discher J. Cell Biol.， 2008，10.1083/jcb.200708043 read abstract Time lapse microscopy of Streptomyces coelicolor growth and sporulation V. Jyothikumar， E. Tilley， R. Wali and P. Herron Appl. Envir.Microbiol.， 2008， 10.1128/aem.01233-08read abstract 活体细胞成像的加热阶段独立控制，被加热的ibidi加热和培养系统的玻璃盖解决了活体细胞成像时产生冷凝的难题。通过加热盖子至一个比金属板高的温度，创建一个垂直温度梯度，这种梯度和活跃的湿度控制防止在培养皿盖上形成冷凝水，在细胞的位置的温度保持在一个恒定的37 °C。ibidi 气体培养系统对氧气和二氧化碳精确可靠的气体培养灵敏和快速加湿-没有蒸发使用加压气体产生气流——无震动可供选择的空气压力发生器（对于不能获取加压空气时）实验样品血管生成研究中的管形成研究显微镜用于调查的管形成过程。根据感兴趣的焦点，这可以通过使用视频显微镜（一个连续的影像），也可以通过观察在不同的时间点的图像来完成。该ibidi加热和培养系统在整个试验期间中在培养箱内提供了卓越的温度稳定性