蛋白质中蛋白质含量分析检测方案(质谱部件及外设)

NANOBAUMETMSP-403K PARTS ASSEMBLY DIAGRAM NANOBAUME HIGHPRESSURE PACKINGFORCAPILLARYCOLUMNS PACK YOUR OWN CAPILLARIES FOR HPLC AND MASS SPEC ANOBAUME NANOBAUME CAPILLARY COLUMN PACKER Pack micro-columns of various length and diameterCompact design will not tip on the work surface Seals safely at pressures up to 9000 psi * Assembles simply，without tools Priced economically Save time， money， and gaincontrolbypackingyyour owncapillary columns.Designed toaccommodate emptyfusedsilica or PEEKsilTM columns of lessthan 1/32” in outer diameter，the NanobaumeTM is a compact316 stainless steel unit built to packtubing with a silica bed at highpressure. lThe NanobaumeTM maybe used for packing at up to9000 psig with liquid media (3000psig for gas pressure) occupies asmall footprint on the benchtop(at 2.5”in diameter， 2.5” tall)， andassembles easily in a few simplesteps. The ETFE O-ring seals at highpressure by hand-tightening only.A magnetic stir bar keeps thesilica slurry suspended over a stir-plate during column packing. Notforuse under SFC conditions. * Packing limit of the pressure vessel is 9000psiwith liquid media； fittings supplied in SP-400 toconnect a column to the Nanobaume will holdpressure to 6000 psi； SP-403K includes high-pressure (9000psi liquid media) CE-005 columnconnection kit. SP-400 NanobaumeTM Assembly SP-403K NanobaumeIM Startup Kit SP-400 NANOBAUMEMASSEMBLY Our base unit - all the components for a functional packerIncludes connections for 360um and 1/32”OD columns Order the SP-400 assembly when all you need is a packer to get started. Includes apressure source connection for 1/16”OD tubing， two column connections each for1/32" and 360 pm OD， glass vial， ETFE O-ring， Teflon"micro stir bar， and SS316 hardware(body， cap lock and cap seal). Note that the SP-400 assembly does not include columnend fittings， which are used to retain the silica bed while packing. Although the pres-sure limit of the packing vessel is 9000 psig with liquid media， the column connectionswill hold to 6000 psig； for higher pressure packing， the CE-005 Adapter Kit is required.These and other items may be purchased separately or as part of the SP-403K Kit. Thefollowing items are included in the SP-400： NANOBAUMETM CAPILLARY PACKING UNITSP-400 Nanobaume High Pressure Capillary Packing Assembly SP-403K STARTUP KIT The ideal choice when starting from scratch- includes theSP-400 Assembly and the most commonly used accessories High Pressure End Fittings and accessories included Rated to 9000 psig with liquid media The SP-403K Startup Kit includes the most common accessories used in capillary columnpacking. At the heart of the system is the SP-400 Nanobaume Packing unit， with pack-ing accessories to connect to a pressure source and monitor the system pressure. Alsoincluded is the CE-005 High Pressure Packing Adapter Kit， which includes end fittingsand column connections capable of 9000 psig packing pressure with liquid media(3000 psig recommended with gas). Below are the items included in the SP-403K： PART # QUANTITY DESCRIPTION SP-400NANOBAUMEPACKING UNITSP-087PRESSURE GAUGE， 0-10000 PSI， SS316SP-0462TUBING， 0.020”ID X 1/16”OD X 10CM， SS316SP-050SHUTOFF VALVE， 3-WAY WITH BOTTOM VENTSP-055TUBING， 0.020”ID X 1/16"ODX5FEET， SS316SP-058PRESSURE GAUGE TEE FOR 1/16"OD TUBINGVL-002XGLASS VIAL， 2ML， 12X32， PACK OF 10CE-005HIGH PRESSURE PACKING ADAPTER KIT NANOBAUME STARTUP KITSP-403K NanobaumeM High Pressure Capillary Packing Startup Kit CE-003 COLUMN END FITTINGS 27nL void volume with 0.5pm Stainless Steel screen For fused silica and 1/32”OD PEEKsilTM columns Rated to 9000 psig with liquid media To successfully pack a capillary column， a fritted end is required to retain the silica bedwhile packing. The CE-003 is a stainless steel end fitting made for direct-connection to1/32”OD columns. Connection to columns and transfer tubing of smaller OD may beachieved by use of the appropriate column adapter sleeve， such as included in the TS-015 Adapter Kit. Use the CE-003 at system pressures up to 9000 psig with liquid media(3000 psig recommended with gas pressure). Please note that when the Nanobaume isin use with CE-003 end fittings， the CE-004 High Pressure Column-To-Nanobaume Con-nection is required at pressure above 6000 psig. CE-003 High Pressure Column End Fitting， SS316， with (1) SP-088 0.5pm frit CE-004 High Pressure Column-To-Nanobaume connection， with (1) SP-094 plug SP-088 Frit， 0.5 stainless steel SP-089 Micro Union， SS316， for 1/32"OD columns， 150pm bore， with 6-40 ports SP-092 Ferrule， for 1/32" OD columns， SS316 SP-094 Pressure testing plug， SS316， 6-40 threads TS-015 Column adapter kit， includes two 1/32"OD PEEK adapters each in five ranges： 125-165pm， 150-190pm，225-265pm， 325-365pm，455-495pm CE-005 COLUMN ADAPTER KIT A 9000 psig conversion kit for the SP-400 base unit Includes sample columns， end fittings， and adapters For fused silica and 1/32”OD PEEKsilM columns For those customers who purchased the SP-400 base unit and later require higher pack-ing pressure， the CE-005 High Pressure Column Adapter Kit will convert the 6000 psig ca-pability of the column connections to 9000 psig with liquid media. Includes two CE-003end fittings with spare SP-092 ferrules and SP-088 frits， sample SP-044 PEEKsil and SP-010fused silica columns， and TS-015 Adapter Kit for columns with OD smaller than 1/32". ACE-004 High Pressure Column-To-Nanobaume Connection and SP-094 pressure-testingplug are also included. HIGH PRESSURE ADAPTER KIT CE-005 High Pressure Column Packing Adapter Kit SILICA BULK MATERIAL Pack columns with the phase of your choice Other packings available-please inquire A number of small particle silicas are available below and on the following page.Select a phase to suit your needs and order by the gram. Other packings are avail-able； please contact your distributor for a broader selection or allow our technicalstaff to suggest a material tailored to your requirements. SILICA BULK MATERIAL SP-008 Strong Cation Exchange， PolySULFOETHYL A， 5pm，300A SP-004 Weak Cation Exchange， PolyCAT A， 5pm， 1000A A Stainless Nut B SP-092 Ferrule SP-089 Union D SP-088 Frit 0G CE-004 COLUMN-TO-NANOBAUME HIGH PRESSURE CONNECTION SILICA BULK MATERIAL， CONTINUED SP-005Hydrophilic Interaction， PolyHYDROXYETHYLA， 5pm， 100ASP-007Hydrophilic Interaction，PolyPROPYL A， 5pm， 300ASP-006C-18， 5pm，300ASP-002C-18，5pm， 100A Hydrophilic EndcapSP-003C-8， 5pm， 100ASP-001C-18， 5pm， 100A FUSED SILICA Methyl-Deactivated and Undeactivated optionsPolyimide coated for strength Fused silica tubing may be used as column bodies or as transfer tubing to connect aPEEKsil column to your system. May be cut to length by the user. Although referred to as“360 micron OD" by the industry， typical tolerances are±10pm on the OD，±3-4pm on theID. Note that Deactivated fused silica has been coated on the ID to reduce active sites，while Un-Deactivated is standard uncoated fused silica. Priced per meter. SP-010 100pm IDx 365um OD， Deactivated fused silica SP-011 250pm ID x350pm OD， Deactivated fused silica UN-DEACTIVATED FUSED SILICA TUBING SP-059 5pm ID x 363m OD， Un-Deactivated fused silica SP-060 10pm ID x363pm OD， Un-Deactivated fused silica SP-061 20pm IDx363pm OD，Un-Deactivated fused silica SP-062 50um ID x 363pm OD，Un-Deactivated fused silica SP-063 75pm IDx363pm OD，Un-Deactivated fused silica SP-064 100pm IDx363um OD， Un-Deactivated fused silica SP-065 200pm ID x363pm OD， Un-Deactivated fused silica SP-066 250pm IDx363pm OD，Un-Deactivated fused silica FUSED SILICA TUBING CUTTER SP-019 Precision fused silica tubing cutter PEEKsiIM EMPTY COLUMNS Fused silica lined PEEK tubing， UndeactivatedAvailable in pre-cut lengths only PEEKsilM is fused silica tubing covered by a PEEK polymer sheath， supplied here in 1/32"OD. Mechanically stronger than fused silica tubing alone， it is readily adaptable totypical stainless or PEEKM tubing connections. PEEKsillM must be factory-cut to length. PEEKSILTM EMPTY COLUMNS SP-031 25pm IDx 1/32" OD， 50mm long， pack of 2 Orange SP-032 25pm ID x 1/32”OD， 100mm long， pack of 2 Orange SP-033 25pm ID x 1/32"OD， 150mm long， pack of 2 Orange SP-034 50pm ID x1/32"OD， 50mm long， pack of 2 Beige SP-035 50pm IDx 1/32"OD， 100mm long， pack of 2 Beige SP-036 50pm IDx1/32"OD， 150mm long， pack of 2 Beige SP-037 75pm IDx1/32"OD， 50mm long， pack of 2 Black SP-038 75pm IDx 1/32"OD， 100mm long， pack of 2 Black SP-039 75pm ID x 1/32"OD， 150mm long， pack of 2 Black PEEKSILTM EMPTY COLUMNS， CONTINUED SP-040 100pm ID x 1/32"OD， 50mm long， pack of 2 RedSP-041 100pm IDx 1/32"OD， 100mm long， pack of 2 RedSP-042 100pm ID x 1/32"OD， 150mm long， pack of 2 RedSP-043 150pm ID x1/32"OD， 50mm long， pack of 2 PurpleSP-044 150pm ID x 1/32”OD， 100mm long， pack of 2 Purple SP-045 150pm ID x 1/32”OD， 150mm long， pack of 2 Purple PARTS AND ACCESSORIES Replacement parts High-pressure tubing and connectionsGauges， valves， and flow control accessories Connection of the NanobaumeM to a pressure supply source requires an adapter andseveral lengths of high-pressure tubing. Inserting an SP-029 pressure gauge into thefluid stream (using the SP-058 gauge tee) will allow monitoring of the packing pressure.A 3-way valve (SP-050) is an efficient way to connect to or remove the unit from thepressure source. SP-052 THREADED INTO SP-400 BODY NANOBAUMETM PARTS AND ACCESSORIES SP-013 Ferrule for 1/32"OD columns (use with SP-014 Nut)，PEEK SP-014 Nut， 10-32， SS316 SP-015 Ferrule for 360um OD columns (use with SP-014 Nut)， Kel-F SP-026 Pressure-Testing Plug， 10-32 threads SP-030 Tubing， PEEK， green， 1/16"OD x 0.030" ID， priced per foot SP-046 Tubing， 316 stainless steel， 1/16"OD x 0.020"IDx10cm long SP-050 Three-Way Valve with bottom vent， SS316 SP-051 Port Adapter， 1/16”OD tube x 1/8"NPT， straight， SS316 SP-052 Port Adapter， 1/16”OD tube x 1/8"NPT， 90° angle， SS316 SP-055 Tubing， 316 stainless steel， 1/16"ODx0.020"ID x 5'long SP-056 Port Adapter Replacement Ferrule， for 1/16" OD tube， SS316 SP-057 Port Adapter Replacement Female Nut， SS316 SP-058 Pressure Gauge Tee， with two 10-32 fittings for 1/16"OD tubing， SS316 SP-085 Nanobaume Stir Bar， 5mm x 2mm， PTFE SP-087 Pressure Gauge， 0-10，000 PSIG， stainless steel， non liquid-filled SP-098 Nanobaume low-pressure Static O-ring， Viton (<1000 psi) SP-300-03 Nanobaume high-pressure Static O-Ring， ETFE with Inconel spring SP-400-02 Nanobaume Cap Seal VL-002X Glass Vial， 2mL， 12x32mm， pack of 10 WARNING： PACKING AT HIGH PRESSURE CAN BE HAZARDOUS.LDONOT USE AT PRESSURE ABOVE RECOMMENDED LEVELS (9000 PSIGLIQUID， 3000 PSIG GAS). NOT FOR SFC (SUPERCRITICAL FLUIDCHROMATOGRAPHY) USE. PURGE AIR FROM LINES BEFORE PACKINGWITH LIQUID MEDIA. NOTE： WE OFFER NO GUARANTEE REGARDING COLUMN-TO-COL-UMN PACKING REPRODUCIBILITY， COLUMN EFFICIENCY (THEORETICALPLATES ACHIEVED)， OR COLUMN SELECTIVITY OR TAILING FACTORS.CAPILLARY COLUMN PACKING IS， AT THIS STAGE， A HIGHLY EXPERI-MENTAL TECHNIQUE. PRICES SUBJECT TO CHANGE WITHOUT NOTICE NanobaumeM is a Trademark of Western Fluids Engineering 8 Mfg， LLCTeflon@， Tefzel， and Delrinare Registered Trademarks of E.l. du Pont deNemours and Company Kel-F@ is a Registered Trademark of the 3M Company PolySULFOETHYL A， PolyCAT A， PolyHYDROXYETHYL A， and PolyPROPYL A are trademarks of PolyLC Inc. PEEKsil" is a Trademark of SGE International Pty. Ltd. PEEK'M polymer is a Trademark of Victrex PLC HIGH PRESSURE PACKINGFORRCAPILLARY COLUMNS PACK YOUR OWN CAPILLARIES FOR HPLC AND MASS SPEC AUGUST 2007 NANOBAUME 800.542.8421[fax]