Fig4: Flow cytometric analysis of E-Cadherin was done on Hela cells. The cells were fixed, permeabilized and stained with E-Cadherin antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.