Anti-Frizzled-5 antibody

Anti-Frizzled-5 antibody

• 种属反应性Human

• 验证应用WB,ICC,FC

• 抗体类型兔多抗

• 免疫原Synthetic peptide within Human Frizzled-5 of aa 168-181.

• 偶联Non-conjugated

• Anti-Frizzled-5 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Peptide affinity purified.

• 亚细胞定位Cell membrane.Golgi apparatus. Membrane. Synapse.

• 数据链接SwissProt: Q13467 Human
  

• 其它名称C2orf31 antibody
  DKFZP434E2135 antibody
  Frizzled 5 seven transmembrane spanning receptor antibody
  Frizzled class receptor 5 antibody
  Frizzled family receptor 5 antibody
  Frizzled homolog 5 antibody
  Frizzled-5 antibody
  Frizzled5 antibody
  Fz 5 antibody
  Fz-5 antibody
  Fz5 antibody
  FZD 5 antibody
  fzd5 antibody
  FZD5_HUMAN antibody
  FzE 5 antibody
  FzE5 antibody
  HFZ 5 antibody
  hFz5 antibody
  MGC129692 antibody
  Seven transmembrane receptor frizzled 5 antibody
  Uncharacterized protein C2orf31 antibody
  Wnt receptor antibody

  more

• Anti-Frizzled-5 antibody应用

  WB:1:500-1:1,000
  ICC:1:50-1:200
  FC:1:50-1:100

• 

  Fig1: Western blot analysis of Frizzled-5 on human ES cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  

  Fig2: ICC staining Frizzled-5 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (0905-3) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig3: ICC staining Frizzled-5 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (0905-3) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig4: Flow cytometric analysis of Frizzled-5 was done on HepG2 cells. The cells were fixed, permeabilized and stained with Frizzled-5 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution.

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