Anti-Prostaglandin E Receptor EP1 antibody
种属反应性Human,Mouse,Rat,Dog
验证应用WB,FC
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human PTGER1/Prostaglandin E Receptor EP1 61-160/402
偶联Non-conjugated
Anti-Prostaglandin E Receptor EP1 antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式affinity purified by Protein A
亚细胞定位Cell membrane; Multi-pass membrane protein.
其它名称Prostaglandin E Receptor EP1
EP1
PGE receptor EP1 subtype
PGE2 receptor EP1 subtype
Prostaglandin E receptor 1 subtype EP1 42kDa
Prostaglandin E receptor 1 subtype EP1
Prostaglandin E2 receptor EP1 subtype
Prostanoid EP1 receptor
PE2R1_HUMAN.
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Anti-Prostaglandin E Receptor EP1 antibody应用
WB:1:500-2000
FC:1ug/Test
Fig1: Sample: Kidney (Mouse) Lysate at 40 ug
Primary: Anti-Prostaglandin E Receptor EP1 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 45 kD
Fig2: Sample: Brain (Rat) Lysate at 40 ug
Primary: Anti-Prostaglandin E Receptor EP1 at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Fig3: Blank control (Black line): Mouse blood (Black).
Primary Antibody (green line): Rabbit Anti-Prostaglandin E Receptor EP1 antibody
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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