Fig2: Citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (ER1919-20) at 1:400 overnight at 4°C, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37°C, followed by a conjugated streptavidin at [1:500] for 40 minutes and DAPI staining of the nu Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium clei.