Fig3: Western blot analysis of YWHAQ against Hela (1), NIH/3T3 (2), C6 (3), A549 (4), COS7 (5), PC-12 (6), and HEK293 (7) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.