种属反应性Human,Mouse,Rat,Pig,Cow,Horse,Sheep
验证应用WB,IHC-P
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human PKC iota/PKCI 181-280/596
偶联Non-conjugated
Anti-PKC iota antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式affinity purified by Protein A
亚细胞定位Cytoplasm. Membrane. Endosome. Nucleus. Transported into the endosome through interaction with SQSTM1/p62. After phosphorylation by cSrc, transported into the nucleus through interaction with KPNB1. Colocalizes with CDK7 in the cytoplasm and nucleus. Vesi
其它名称aPKC lambda/iota
aPKC-lambda/iota
Atypical protein kinase C lambda/iota
Atypical protein kinase C-lambda/iota
DXS1179E
KPCI_HUMAN
MGC26534
nPKC iota
nPKC-iota
nPKCiota
PKC lambda
PKCI
PKCiota
PKClambda
PRKC iota
PRKC lambda/iota
PRKC-lambda/iota
PRKCI
Protein kinase C iota
Protein kinase C iota type.
more
Anti-PKC iota antibody应用
WB:1:500-2000
IHC-P:1:400-800
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PKC iota) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig2: Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti-PKC iota at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kD
Observed band size: 68 kD
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