Anti-TNNT2 antibody
种属反应性Human,Mouse
验证应用WB,ICC,IHC-P,FC
抗体类型小鼠单抗
免疫原Synthetic peptide within human TNNT2 50-100 aa.
偶联Non-conjugated
Anti-TNNT2 antibody性能
形态Liquid
浓度2 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG2b
纯化方式Peptide affinity purified
亚细胞定位Cytoskeleton. Cytosol.
其它名称
more
Cardiac muscle troponin T antibody
Cardiomyopathy dilated 1D (autosomal dominant) antibody
Cardiomyopathy hypertrophic 2 antibody
Anti-TNNT2 antibody应用
WB: 1:2,000-1:5,000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:100
Fig1: Western blot analysis of TNNT2 on human heart tissue lysate using anti-TNNT2 antibody at 1/2,000 dilution.
Fig2: ICC staining TNNT2 (red) in SK-Br-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-TNNT2 antibody. Counter stained with hematoxylin.
Fig4: Flow cytometric analysis of SK-Br-3 cells with TNNT2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
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