Anti-GPC3 antibody
种属反应性Human,Mouse
验证应用WB,ICC,IHC-P,FC
抗体类型小鼠单抗
免疫原Recombinant protein
偶联Non-conjugated
Anti-GPC3 antibody性能
形态Liquid
浓度2 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
亚型IgG1
纯化方式Protein A purified.
亚细胞定位Plasma membrane. Secreted.
其它名称
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Anti-GPC3 antibody应用
WB: 1:500-1:1,000
ICC: 1:100-1:500
IHC-P: 1:100-1:500
FC: 1:100-1:200
Fig1: Western blot analysis of Glypican3 on human Glypican3 recombinant protein using anti- Glypican3 antibody at 1/1,000 dilution.
Fig2: Western blot analysis of Glypican3 on different lysates using anti- Glypican3 antibody at 1/1,000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: HEK293
Lane 3:Jurkat
Lane 4: SK-N-SH
Lane 5: PC-12
Lane 6: F9
Lane 7: Mouse liver tissue
Fig3: ICC staining Glypican3 (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded liver cancer tissue using anti- Glypican3 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded breast cancer tissue using anti- Glypican3 antibody. Counter stained with hematoxylin.
Fig6: Flow cytometric analysis of Jurkat cells with Glypican3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
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