Anti-Glutathione peroxidase 1 antibody
种属反应性Human,Mouse
验证应用WB,ICC,FC
抗体类型小鼠单抗
免疫原Recombinant protein within human GPX1 aa 30-203.
偶联Non-conjugated
Anti-Glutathione peroxidase 1 antibody性能
形态Liquid
浓度2 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG2b
纯化方式Protein G affinity purified.
亚细胞定位Cytoplasm.
其它名称
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Anti-Glutathione peroxidase 1 antibody应用
WB: 1:500-1:2,000
ICC: 1:50-1:100
FC: 1:50-1:100
Fig1: Western blot analysis of Glutathione peroxidase 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: THP-1 cell lysate
Lane 2: 293 cell lysate
Fig2: ICC staining of Glutathione peroxidase 1 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Fig3: Flow cytometric analysis of Glutathione peroxidase 1 was done on THP-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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