Anti-C3a Receptor antibody
种属反应性Human,Mouse,Rat,Horse,Rabbit
验证应用WB,IHC-P
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human C3a Receptor/C3aR 401-482/482
偶联Non-conjugated
Anti-C3a Receptor antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式affinity purified by Protein A
亚细胞定位Cell membrane; Multi-pass membrane protein.
数据链接SwissProt: Q16581 Human
其它名称AZ 3B
AZ3B
C3a anaphylatoxin chemotactic receptor
C3A R
C3A R1
C3a-R
C3AR
C3AR_HUMAN
C3AR1
C3R1
Complement component 3 receptor 1
Complement component 3a receptor 1
HNFAG09.
more
Anti-C3a Receptor antibody应用
WB:1:500-2000
IHC-P:1:400-800
Fig1: Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (C3a Receptor) Polyclonal Antibody, Unconjugatedat 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig2: Sample:
K562(Human) Cell Lysate at 30 ug
293T(Human) Cell Lysate at 30 ug
A549(Human) Cell Lysate at 30 ug
Lung (Mouse) Lysate at 40 ug
Primary: Anti-C3a Receptor at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 64 kD
Fig3: Sample: A549 Cell (Human) Lysate at 30 ug
Primary: Anti-C3a Receptorat 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54kD
Observed band size: 60kD
Fig4: Sample: 293T Cell (Human) Lysate at 30 ug
Primary: Anti-C3a Receptorat 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54kD
Observed band size: 60kD
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