Anti-cMet antibody

Anti-cMet antibody

• 种属反应性Human, Mouse, Rat

• 验证应用WB, ICC, IHC-P, FC

• 抗体类型兔多抗

• 免疫原Synthetic peptide within mouse cMet aa 650-690.

• 偶联Non-conjugated

• Anti-cMet antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Peptide affinity purified

• 亚细胞定位Cell membrane.

• 其它名称AUTS9 antibody
  c met antibody
  D249 antibody
  Hepatocyte growth factor receptor antibody
  HGF antibody
  HGF receptor antibody
  HGF/SF receptor antibody
  HGFR antibody
  MET antibody
  Met proto oncogene tyrosine kinase antibody
  MET proto oncogene, receptor tyrosine kinase antibody
  Met proto-oncogene (hepatocyte growth factor receptor) antibody
  Met proto-oncogene antibody
  Met protooncogene antibody
  MET_HUMAN antibody
  Oncogene MET antibody
  Par4 antibody
  Proto-oncogene c-Met antibody
  RCCP2 antibody
  Scatter factor receptor antibody
  SF receptor antibody
  Tyrosine-protein kinase Met antibody

  more

• Anti-cMet antibody应用

  WB: 1:1,000
  ICC: 1:200
  IHC-P: 1:200
  FC: 1:100-1:200

• 

  Fig1: Western blot analysis of cMet on different cell lysates using anti-cMet antibody at 1/1000 dilution.
  Positive control:
  Lane 1: Mouse liver
  Lane 2: Mouse kidney
  Lane 3: D3
  Lane 4: MEF

  

  Fig2: ICC staining cMet in N2A cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig3: ICC staining cMet in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig4: ICC staining cMet in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig5: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-cMet antibody. Counter stained with hematoxylin.

  

  Fig6: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-cMet antibody. Counter stained with hematoxylin.

  

  Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-cMet antibody. Counter stained with hematoxylin.

  

  Fig8: Flow cytometric analysis of Hela cells with cMet antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.

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