Anti-Bcl-2 antibody
种属反应性Human
验证应用WB,ICC,IHC-P,FC
抗体类型兔多抗
免疫原Synthetic peptide (KLH-coupled) within human Bcl-2 aa 20-80.
偶联Non-conjugated
Anti-Bcl-2 antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Peptide affinity purified
亚细胞定位Mitochondrion outer membrane, nucleus membrane
数据链接P10415
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Anti-Bcl-2 antibody应用
WB: 1:500
ICC: 1:200
IHC-P: 1:200
FC: 1:50-1:100
Fig1: Western blot analysis of Bcl-2 on different cell lysates using anti-Bcl-2 antibody at 1/500 dilution.
Fig2: ICC staining Bcl-2 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Bcl-2 rabbit polyclonal antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Bcl-2 rabbit polyclonal antibody. Counter stained with hematoxylin.
Fig5: Flow cytometric analysis of Hela cells with Bcl-2 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
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