Anti-GPS2 antibody
种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Sheep
验证应用WB,FC
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human GPS2 1-100/327
偶联Non-conjugated
Anti-GPS2 antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式affinity purified by Protein A
亚细胞定位Testis (Mouse) Lysate, A549.
其它名称AMF-1
G protein pathway suppressor 2
GPS-2
GPS2
MGC104294
MGC119287
MGC119288
MGC119289
GPS2_HUMAN.
more
Anti-GPS2 antibody应用
WB:1:500-2000
FC:1ug/test
Fig1: Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti- GPS2 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37kD
Observed band size: 37kD
Fig2: Blank control: A549.
Primary Antibody (green line): Rabbit Anti-GPS2 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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