Anti-Phosphoserine antibody
种属反应性Human,Mouse,Rat
验证应用WB,IHC-P
抗体类型小鼠单抗
免疫原Purified Protein
偶联Non-conjugated
Anti-Phosphoserine antibody性能
形态Liquid
浓度1 mg/mL
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
亚型IgG1
纯化方式The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
其它名称
phospho-Ser antibody
pS antibody
pSER antibody
Anti-Phosphoserine antibody应用
WB:1:500-1:2,000
IHC-P:1:50-1:200
Fig1: Western blot analysis of Phosphoserine on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: Rat brain lysate
Lane 3: Mouse brain lysate
Fig2: Immunohistochemical analysis of paraffin-embedded Human Lung Caricnoma tissue using anti-Phosphoserine antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Fig3: Immunohistochemical analysis of paraffin-embedded Human Breast Caricnoma tissue using anti-Phosphoserine antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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