摘要 本研究使用一种大范围疑似物筛查工作流程，来鉴定废水出水中的有毒化学物质。该全面的分析方法将靶向和非靶向方法相结合，使用在多种电离模式下运行的高分辨率精确质量数 Agilent 7250 GC/Q-TOF、Agilent MassHunter 定量分析软件 10.1 中的 GC/Q-TOF 筛查工作流程以及农药和环境污染物的 GC/Q-TOF 精确质量谱库。

前言 对食品中污染物的筛查需求不断增长，这时需要一种高效灵敏的技术[1]。高分辨率 GC/Q-TOF 成为了满足这一目标的技术，用于适合气相色谱分析的化合物。相同的全谱精确质量数据有助于对样品中的化合物进行可靠的鉴定，其定量分析能力也满足严格的最大残留限量 (MRL) 要求。低能量电子轰击 (EI) 离子源的加入增强了在 EI 质谱图中保留或确认分子离子的可能性，有助于研究未知物。本文介绍了如何使用支持低能量 EI 的新型高分辨率 GC/Q-TOF 筛查食品基质中的农药及其他污染物。

Abstract This application note describes the use of the novel simultaneous dynamic multiple reaction monitoring (dMRM) and scan (dMRM/scan) data acquisition mode for triple quadrupole gas chromatography mass spectrometry (GC/TQ) analysis of pesticides in challenging food matrices. The simultaneous dMRM/scan capability enables identification of the unknown compounds and retrospective analysis, while maintaining sensitivity and dynamic range of the method comparable to a conventional dMRM analysis. Additionally, scan data enables more confidence in compound identification by library spectrum matching. Finally, the full scan data allow the analyst to evaluate the sample matrix to ensure the most efficient performance of the GC/TQ system. This work demonstrates the application of dMRM/scan to the analysis of extracts, using Agilent QuEChERS sample preparation, of spinach, walnut, and cayenne pepper spiked with over 200 pesticides. The calibration results and method sensitivity for 203 evaluated compounds were comparable to results observed with conventional dMRM data acquisition mode with the Agilent 8890/7000E GC/TQ and the Agilent 8890/7010C GC/TQ. The unknown identification workflow based on the spectral library matching using a retention time locked library was carried out with Agilent MassHunter Unknowns Analysis. Many of the compounds with the established maximum residue limits (MRLs) were identified with full scan data at concentrations below their MRLs even in the challenging cayenne pepper extract

Abstract This application note illustrates a sensitive method used to analyze semivolatile organic compounds (SVOCs) on an Agilent 7000E triple quadrupole GC/MS system (GC/TQ). The use of GC/TQ instrumentation for analysis of SVOCs offers significant advantages. High selectivity afforded by multiple reaction monitoring (MRM) mode results in faster batch review and increased confidence due to the elimination of matrix interferences. These interferences are often present when using selective ion monitoring (SIM) or scan acquisition modes. Increased sensitivity can facilitate smaller extraction volumes that improve sustainability, reduce waste, and decrease costs associated with sample preparation, solvent usage, and waste disposal. A primary objective of this work was to demonstrate the ability of a GC/TQ to detect SVOCs at low levels to meet these laboratory needs while maintaining an excellent dynamic range.

Abstract This application note describes two approaches for achieving robust, multiresidue pesticide analysis in 10 minutes by GC/MS/MS, while maintaining sufficient chromatographic resolution for the analysis of over 200 pesticides in spinach; a challenging high chlorophyll, fresh matrix. First, the conventional 15 × 15 m (0.25 mm × 0.25 μm) midcolumn backflush configuration was used with an accelerated oven ramp, yielding an analysis time of 10 minutes. Second, a minibore 10 × 10 m (0.18 mm × 0.18 μm) midcolumn backflush configuration was used, enabling a fast 10-minute analysis time. The latter method was precisely scaled using the Agilent GC method translation technique. It was shown that midcolumn backflushing enabled method robustness and extended maintenance-free operation of the system by minimizing column trimming and source cleaning. Results demonstrate that the Agilent 7000E and 7010C triple quadrupole GC/MS systems delivered excellent linearity over a concentration range of 0.1 to 1,000 parts per billion (ppb). Method robustness was shown with 700 consecutive injections of a spinach extract, spiked with pesticides at 20 ppb, that spanned over 175 hours of continuous running of the GC/TQ.

Using the Agilent HydroInert source in a challenging soil matrix

Abstract This application note describes the key strategies for pesticide analysis with gas chromatography/triple quadrupole mass spectrometry (GC/TQ) using hydrogen as the carrier gas while maintaining sensitivity to meet maximum residue limits (MRLs). The key aspects addressed in this work include the recommended column configuration, the optimized injection conditions, and the appropriate choice of the mass spectrometer (MS) electron ionization (EI) source hardware developed for use with hydrogen carrier gas. The 20 m × 20 m (0.18 mm × 0.18 μm) Agilent HP-5ms UI midcolumn backflush configuration allowed for maintaining the same retention times as with helium, leading to time savings associated with method translation. The resulting chromatographic resolution achieved under the optimal conditions with hydrogen surpassed that with helium. The optimized injection conditions included solvent vent mode, a 2 mm dimpled liner, and the use of analyte protectants. The analyte response with hydrogen was enhanced on average 10-fold when using the optimized conditions compared to using hydrogen carrier gas with the injection conditions, commonly used with helium. Both the Agilent HydroInert and the Agilent High Efficiency Source (HES) resulted in nearly identical spectra bserved with hydrogen and helium, which allowed using the same multiple reaction monitoring (MRM) transitions and collision energies as with helium. The ability to use the same MRMs, collision energies, and retention times greatly simplified the transition from helium to hydrogen.

Abstract Gas chromatography/mass spectrometry (GC/MS) is integral to the analysis of semivolatile organic compounds (SVOCs) in environmental matrices. Recent pressure on the helium (He) supply has required organizations to actively investigate hydrogen (H2) carrier gas, but most GC/MS analyses have reduced sensitivity and hydrogenation or dechlorination in the sources. The Agilent HydroInert source retains the ability to analyze a wide calibration range (0.1 to 100 μg/mL) and meet the U.S. Environmental Protection Agency (EPA) method 8270 calibration criteria when using H2 carrier gas.

Abstract An Agilent 8890/5977C GC/MSD system coupled with an Agilent 8697 headspace sampler was successfully used with hydrogen carrier gas for the analysis of volatile organic compounds (VOCs) in drinking water. Recent concerns with the price and availability of helium have led laboratories to look for alternative carrier gases for their GC/MS methods. For GC/MS, hydrogen is the best alternative to helium, and offers potential advantages in terms of chromatographic speed and resolution. However, hydrogen is not an inert gas, and may cause chemical reactions in the mass spectrometer electron ionization (EI) source. This can lead to disturbed ion ratios in the mass spectrum, spectral infidelity, peak tailing, and nonlinear calibration for some analytes. Therefore, a new EI source for GC/MS and GC/MS/MS was developed, and optimized for use with hydrogen carrier gas. The new source, named HydroInert, was used in the system evaluated here. In addition to the new source, the chromatographic conditions were optimized to provide separation of 80 volatile compounds in 7 minutes. Standards and samples were analyzed in both scan and SIM data acquisition modes. For the scan data, spectra were deconvoluted with MassHunter Unknowns Analysis software and searched against NIST 20 to assess the spectral fidelity. In both modes, quantitative calibration was performed for the 80 compounds over the range of 0.05 to 25 μg/L. As demonstrated in this note, the system gives excellent results for the analysis of VOCs in drinking water.

Abstract This application note evaluates the performance of an Agilent 8890/5977C gas chromatography/mass selective detector (GC/MSD) in the analysis of semivolatile organic compounds (SVOCs) at both traditional and enhanced sensitivity levels. The GC/MSD system was initially calibrated with a range of 0.2 to 150 μg/mL with 97% of the 76 evaluated analytes meeting the requirement for average response factor (RF) curve fits. Method modifications to enhance sensitivity were applied to challenge a lower calibration range of 0.01 to 10 μg/mL. Under these amended conditions, 97% of the tested compounds also met or exceeded the United States Environmental Protection Agency (US EPA) Method 8270E criteria for average RF fits. These results demonstrated a potential for lower detection of analytes.

Introduction The term photobleaching refers to loss of fluorescence emission from samples due to prolonged exposure to exciting radiation. While many fluorophores retain stable fluorescence after extended periods of illumination, some photobleach after short periods of time. In order to maintain optimal fluorescence emission, it is important to minimize photobleaching. Strategies to achieve this usually involve limiting one or both of the following: (1) the time of exposure to, or (2) the intensity of, the exciting light. However, either of these strategies may compromise the quality of the results or limit the types of analyses that can be performed because the signal to noise ratio (S/N) is unavoidably decreased. Furthermore, kinetics-based assays performed over an extended period of time may not be possible due to increased exposure of the fluorophore to the excitation light which results in photobleaching.

安 捷 伦 是 您 分 子 光 谱 领 域 的 重 要 资 源 和 合 作伙伴 。 世 界 闻 名 的 C a r y 产 品 线 包 括 F T I R 、UV-Vis-NIR 和荧光光谱仪，为您提供全系列的分子光谱解决方案。

用于药物发现、药物开发和药物生产的分析工作流程

简化分析、改善工作流程并节省成本

使用荧光检测辅助体积排阻色谱分析可提高分析效率

Complying with environmental standards Metal plating is a process that uses electrochemical methods to treat material surfaces. As the process varies with the material to be plated, the pollutants in the liquid waste, and their concentrations, vary considerably. Plating wastewater is generally complex in composition, often containing elements such as Cr, Cd, Pb, Cu, Zn, and Ni.

Ensuring the quality of steel Steel manufacturers test the quality of their products by measuring several elements in the steel to ensure the grade and performance of their final product. Different grades of steel have different specifications for elemental content.

前言 由于母婴饮食中硒的缺乏，导致一些新生儿血清样品中的硒含量仅处于 20 μg/L 的痕量水平 [9]。 此前业内已发表了大量有关通过 GFAAS 测定血液和血清中的硒的分析方法 [1,5,6,7,9]。本研究介绍了新仪器和程序的应用，这对于获得最高准确度和精密度非常重要。

摘要 本文介绍了采用安捷伦快速序列火焰原子吸收光谱仪同时测定土壤中铬 (Cr)、铜 (Cu)、镍(Ni) 和锌 (Zn) 元素的方法，对多种土壤成分分析标准物质 GSS-4、GSS-8、GSS-9、GSS-11、GSS-13、GSS-21、GSS-23、GSS-24 中 Cr、Cu、Ni 和 Zn 元素进行验证测试，测定值在 标准物质标准值范围内。该方法操作简便，方法检测限完全满足《土壤环境质量 农用地土壤污染风险管控标准 》的分析要求，适用于土壤中多元素的快速测定。

安捷伦 AA 系列光谱仪 高效、精确、可靠 安捷伦 AA 系列具有高效、易用和极其可靠的特性。该系列产品具有适用于任何分析所需要的高性能，并且同样适用于重视可靠性和易用性的常规实验室 。

Measured using the Agilent 280Z graphite furnace atomic absorption spectrometer and Omega platform tube

Efficient elemental analysis of food samples

High throughput analysis

Heavy metal pollution Heavy metal pollution is a serious environmental problem. Heavy metals enter soil through wastes, irrigation water, air pollution, and settling of airborne particles. By migrating and accumulating in crop products, they can work their way through the food chain.

在正确时间了解正确信息 信息可以帮助您突破日常事务中复杂性的束缚，包括样品、仪器、法规需求以及时间和资源上的其他需求 ― 并帮助您应对意料之外的挑战。分析解决方案可以提供的最重要价值，就是使您确信，在任何有需要的时候您均能得到可靠的答案。基于全球客户的反馈和建议，安捷伦开发了涵盖仪器、消耗品、标准品、服务、软件等多个方面的创新原子光谱产品组合，为您的实验室带来从容应对的信心。

中国 HJ 781-2015 方法概括了采用四酸微波消解法进行样品前处理的固体废物样品中 22 种元素的测定程序。这种复杂样品消解物中通常包含方法的非靶向元素。如果不考虑这些额外元素（通常是未知元素）的存在，则可能导致光谱干扰。光谱干扰可能导致结果错误并迫使样品重新测量，从而造成时间浪费。

Agilent 5800 VDV ICP-OES 智能功能可最大程度减少 样品重新测量

Quality control of unrefined cathode-metal solutions from recycled LIBs by Agilent 5800 VDV ICP-OES

Quality assurance of lithium-ion battery precursor chemicals by Agilent 5800 VDV ICP-OES

Analysis of cathode raw materials for Li-ion batteries by Agilent 5900 ICP-OES