This Application Note describes both HPLC and UHPLC methods to simultaneously separate and identify seven tannin compounds as aging markers in barrel-aged alcoholic beverages. The proposed methodology was applied to four different matrixes: whisky, brandy, rum, and tequila, to demonstrate a correlation between aging time and total tannin concentration level. Such a methodology could easily lead to an authenticity verifi cation method to be implemented at the manufacturing process as a quality control measurement, preventing frauds or adulteration.

This Application Note describes a UHPLC/Q - TOF/MS method for the detection of 240 pesticides and pesticide metabolites in diffi cult food matrixes using the Agilent All Ions MS/MS technique. The method benefi ts from increased chromatographic resolution using the Agilent 1290 Infi nity UHPLC system and improved ionization capa bilities with Agilent Jet Stream ionization source. More importantly, the successful screening and quantitation are accomplished by the innate sensitivity improvement of the Agilent 6545 Q - TOF LC/MS system and ion transmission tuning to facilitate the o ptimal ion transmission of small fragile organic molecules. The All Ions MS/MS technique features the easy acquisition method setup and retrospective data interrogation with expanded library. Black tea matrix was chosen for its complexity, and avocado m atrix was chosen to represent food commodities with high lipid content. Our results demonstrate that the improved detection of small organic molecules by the 6545 Q - TOF LC/MS system allows the screening and quantitation of most of the targeted pesticid es below the maximum residue limits (MRLs) specifi ed by regulations

The Agilent 1290 Infinity II LC was used to determine the bitter compounds iso-alpha-acids and reduced iso-alpha-acids, and the clove-like phenolic flavor 4-vinylguaiacol in bottled beer. A six-minute UHPLC method was developed to measure these important solutes in a single run. Diode array and fluorescence detectors (DAD and FLD) were placed in-series for quantification of iso-alpha-acids and reduced iso-alpha-acids, and of 4-vinylguaiacol, respectively. The fluorescence detector provided a selective, sensitive, and accurate determination of ppb amounts of 4-vinylguaiacol. Some figures of merit are presented together with the profiles of a series of different beers.

The 5100 Synchronous Vertical Dual View (SVDV) ICP-OES has a range of features that simplify the analysis and sample preparation procedures required for the Chinese National Food Safety Standard (GB 5413.21—201) for measuring Ca, Fe, Zn, Na, K, Mg, Mn and Cu in milk powder for infants.

The Agilent 1290 Infi nity II LC was used to analyze additives such as sweeteners, preservatives, and caffeine in various food products, beverages, and consumer toothpaste. The developed method facilitates accurate and sensitive determination of nine additives using a diode array detector (DAD) and an evaporative light scattering detector (ELSD) placed in series. After the DAD, a valve was installed to remove nonretained solutes in reversed-phase LC, which could increase the noise level of the ELSD. Total analysis time, including re-equilibration, was below 10 minutes with minimal sample preparation. Method performance was evaluated with standard solutions as well as with a series of real samples.

The determination of pesticide residues in food products is important especially for infants and children, as the quantity of food they ingest per kilogram of body weight is relatively high. Some pesticides are known or suspected endocrine disrupting chemicals (EDCs), so the potential for interference with the function of the naturally occurring hormones can severely impact a growing child, even at low concentrations. The implications of long-term, low-dose exposure through food consumption have led to interest in development of highly sensitive analytical methods that combine rapid sample preparation with hyphenated separation and detection. Most pesticide residue laboratories use some variation of the QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) extraction methods. Often the extracted material is analyzed using gas chromatography-tandem mass spectrometry (GC/MS/MS) for the thermally stable, less polar pesticides, or liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis for the less volatile and/or more polar ones. A more recently developed alternative technique involves coupling GC to triple quadrupole ICP-MS (GC-ICP-QQQ), which is able to Determination of pesticides in foods using phosphorus and sulfur detection by GC-ICP-QQQ Application note Authors Jenny Nelson1-3, Helene Hopfer1-2,4, Fabio Silva3 , Steve Wilbur3 , Jianmin Chen3 , Kumi Shiota Ozawa3 and Philip L. Wylie5 1 Department of Viticulture and Enology, University of CaliforniaDavis, Davis CA, USA 2 Food Safety and Measurement Facility, University of CaliforniaDavis, Davis CA, USA 3 Agilent Technologies, Inc., Santa Clara CA, USA 4 HM Clause, Davis CA, USA 5 Agilent Technologies, Inc., Wilmington DE, USA Food testing 2 determine pesticides by measuring the heteroatoms P and S (also Cl and Br) contained in most pesticides. GC-ICP-QQQ offers very good selectivity and specificity, and sensitivity that can be greater than the established methods. The method described in this work ca

Concern about the impact on public health from potentially toxic elements and compounds present in everyday foodstuff has led to new legislative guidance. The inorganic forms of arsenic (As) are known to be toxic and carcinogenic to humans, and food and drink are a potential source of exposure [1]. To reduce the intake of inorganic arsenic from the diet, the U.S. Food and Drug Administration (FDA) proposed a new action level for inorganic arsenic in apple juice of 10 μg/L in 2013 [2,3]. Also, following the eighth meeting of the CODEX Committee on Contaminants in Food, the World Health Organization (WHO) proposed a guideline for inorganic As in polished rice of 0.2 mg/kg [4]. There is a clear need for reliable screening methods to accurately determine the levels of such contaminants in food and drink products. The detection limits required are low, as a 0.2 mg/kg limit equates to a digestate concentration of 2 μg/L (assuming a 100x digestion dilution). Even small errors in the precision and accuracy of the analytical method could result in a food product being wrongly classified as safe. Selenium (Se) is an essential micronutrient that can be deficient in the diet as Se-poor soils yield Se-poor food crops. Accurate quantification of Se in food is necessary to assess nutrient status.

A fast and sensitive method using hydride generation (HG) with ICP-MS has been developed for the separation and detection of inorganic arsenic (iAs) in foodstuffs. The iAs measured values in 31 commercial rice products all fell below the current legislative maximum level (ML) of 150 μg/kg set by China. Comparing the results obtained by HG-ICP-MS and HPLC-ICP-MS showed comparable limits of detection were achieved. HG-ICP-MS is ideally suited to the large scale screening of food samples, such as rice, as analysis can be performed in a fraction of the time typically required by a conventional HPLC-ICP-MS approach, with no compromise in analytical performance.

Milk and milk-based products are an important source of nutrients in the human diet, especially for infants and children. Milk products are widely consumed in many parts of the world. As tastes change and incomes rise, the popularity of dairy products in many Asian and developing countries is rising. With increased production to meet the growing demand, product quality is important. The levels of major elements such as Na, K, Mg, Ca, and essential elements such as Se, P, Mn, Zn, are measured to provide valuable nutritional information. Potentially toxic elements such as As, Cd, Sn, Hg and Pb are also measured in animal-derived milk, to monitor potential contamination from soil, fertilizer, animal feed, or processing equipment. Agilent ICP-MS instruments are widely used in environmental and food testing laboratories due to their exceptional sensitivity, fast multi-elementanalysis and wide elemental coverage. Recent developments have extended the analytical dynamic range of the Agilent 7900 ICP-MS to more than ten orders of magnitude, allowing routine measurement of major elements (such as Na, K and Ca) in milk, in the same analytical run as the trace elements.

Red wine produced from the Malbec grape is increasing in popularity in the United States. However, the US is a relatively small producer of the wine compared to Argentina, where it is the most extensively planted grape variety in the country. With rising imports into the US from Argentina there are growing concerns relating to the validation of the geographical origin of this wine. Typically ICP - M S is used to distinguish between wines originating from different regions by comparing the relative concentrations of mineral elements, which are characteristic of the soil composition of the region of production. In this study, a cost - effective approac h has been taken using Microwave Plasma - Atomic Emission Spectroscopy (MP - AES) to measure 6 elements (Sr, Rb, Ca, K, Na and Mg). Agilent’s Mass Profiler Professional (MPP) integrated chemometrics software and another data analysis package were used to m odel the MP - AES results to distinguish the geographical origin of 41 Malbec wine samples produced in Argentina and the USA.

Milk is one of the most important food commodities in the world and its consumption has grown, particularly in developing countries which have exp erienced strong economic growth and urbanization in recent decades. As a substantial source of several nutrients such as pr oteins, enzymes, fats, vitamins and essential elements (also known as minerals), mi lk plays a key role during all phases of human life. Rapid growth during infancy and early childhoo d creates high demand for the nutrients that milk provides. This development phase requir es a balanced amount of different elements, as mineral deficiencies may impair body devel opment whilst excessive mineral intake may increase the osmotic load and cause complicati ons in the developing kidneys of a child. Essential elements such as Ca, K, Mg, Na and P have several physiological functions in the tissue struct ure of humans and other animals, su ch as maintaining osmotic/electrolyte balance, and acting as a cofactor for many enzymes. Deficiencies in these essenti al elements causes disturbances

为保证获得优质的食品，您必须精确地检测出低含量的变质食品和掺假物并确认食品的来源及类别。 Agilent GC/MS、LC/MS 和 ICP-MS 仪器配备有功能强大的 Mass Profiler Professional 软件，可让您实现： 1，对食品样品高效、可靠的分析 2，高度自动化地运行单个软件平台 3，使用简单的方法对食品样品进行质谱分析

Cereal-based and sucrose-coated infant cereals were ground, and the sucrose, glucose, and fructose concentrations were determined with the Agilent Cary 630 Fourier transform mid-infrared, FTIR, instrument and compared to values measured by HPLC. The results indicate that use of the portable FTIR instrument was in line with the HPLC measurements, and that the Cary 630 is suitable for real-time analysis of sugar concentration in breakfast cereals.

The degree of substitution for sulfobutyl ether b-cyclodextrin (SBE-b-CD) has historically been characterized by nuclear magnetic resonance spectroscopy, but this approach provides gross values for the degree of substitution. The capillary electrophoresis method described here resolves the mixture of positional and regional isomers based on the degree of sulfo butyl ether substitution. Indirect UV detection has been used, where the detection of cyclodextrin is accomplished by a decrease in background absorbance of the background electrolyte buffer benzoic acid. Ten peaks of SBE-b-CD were well resolved with an optimum pH of 8.0 using an Agilent 50 μm id, 56 cm length bare fused silica capillary. The system suitability requirements were passed as per United States Pharmacopeia (USP), and acceptence criteria for averge degree of substitution 6.2 to 6.9 were met.

多环芳烃(PAH) 由稠合苯环体系组成，因此难以降解。它们通过在环境中的积聚以及烟熏等烹饪方法进入水生生物体内。通常高脂肪含量复杂食品基质中的PAH 分析极具挑战性，因为共萃取出的基质会以干扰物、基质效应和分析流路中的累积等形式影响定量分析的准确性。Agilent Bond Elut QuEChERS EMR-Lipid 增强型脂质去除产品为新一代样品前处理产品，可用于便捷的分散固相萃取(dSPE)中，实现高度选择性的基质去除且不影响分析物回收率。本文证明了这一样品前处理方法在进行三文鱼体内PAH分析中的有效性。此方法为各种浓度下的全部15种PAH化合物提供了出色的准确度（回收率为84% - 115%）和精密度（RSD 为0.5% - 4.4%），因此能够快速、稳定而有效地分析高脂肪含量样品。

众所周知，砷 (As) 是一种有毒元素，可存在于环境和食品中。因此，多个国家 和地区对其进行了严格监管。然而，由于砷的生物毒性在很大程度上取决于其化 学形态，因此砷形态分析比总砷分析更为重要。例如，如果已知一种海藻样品含 有高浓度砷，但主要以 AsB 形式存在，那么食用该样品就没有潜在风险，因为 AsB 是无毒的。在砷的五种主要形态 As(V)、MMA、As(III)、DMA 和 AsB 中， 只有两种无机形态（As(III) 和 As(V)）是有毒的。这些无机砷还对人体有致癌作 用，因此尤其需要对食品中的无机砷进行测定。以大米为主食的国家对大米中的 砷特别关注。水稻可从土壤和水中吸收砷。与其他农作物相比，水稻的生长需要 大量的水，因此更可能发生砷积聚。 本研究展示了使用配备 Agilent ZORBAX SB-Aq 色谱柱的 Agilent 1260 HPLC 系统与 Agilent 7900 ICP-MS 联用对大米中有毒砷形态的快速测定方法。

采用 Agilent 7000 系列三重四极杆气质联用系统中的一种完善的分析方法来验证 7000C三重四极杆气质联用系统的性能。在李子和冬南瓜的基质中加标 110 种浓度为 1 ng/g 的农药，这两种复杂基质中各有 92 种农药,其计算的 RSD 值 ~20% (n = 5)。据估算，这两种商品中均有 91% 的农药定量限 (LOQ) 可以达到 ~5 ng/g 的水平。这表明本系统在低于欧盟和日本规定的最大残留限量 (MRL) 0.01 mg/kg (10 ng/g) 的检测水平下，所采集的数据可用于暴露监测。

采用Agilent 1290 UHPLC–三重四极杆6460 串联质谱开发了一个超高灵敏度的、可同时对食物中的全氟辛烷磺酸 (PFOS) 和全氟辛酸 (PFOA) 进行定量分析的方法。样品首先用甲醇提取，然后用弱阴离子交换小柱进行净化。所得溶液采用反相UHPLC 进行分离，并且采用三重四极杆质谱在MRM 模式下进行检测。PFOA 和PFOS 的动态线性范围分别为0.01–10 ng/mL 和0.1–40 ng/mL，线性相关回归系数不小于0.999。PFOA 和PFOS 的检测限 (LOD)［信噪比(S/N) = 3）］分别为0.002 和0.02 μg/kg，其定量限 (LOQ) (S/N =10) 分别为0.01 和0.1 μg/kg。鱼、虾和蛋类基质中三个浓度水平加标的平均回收率在79.2%–113.1% 范围内，其相对标准偏差 (RSD) 在3.2%–10.7% 范围内 (n=6)。实验室间验证进一步确证了方法的准确度和可靠性。所建立的方法具有高灵敏度、高回收率以及良好的选择性等优势。因此，它可用于动物源性食品中PFOA 和PFOS 的常规检验。

食品中潜在有毒元素和化合物的存在日益受到公众的密切关注，因此，食品生产者及管理部门就要求有快速、可靠的筛选方法来准确定量食品和饮料中这些污染物的含量。对于砷 (As) 而言，它在食品中的富集浓度可能会通过以往含砷农药如砷酸氢铅（砷酸铅）或砷酸钙的使用而上升。这些化合物作为收获前农药在20 世纪广泛用于控制水果类作物（主要是苹果）上的害虫，如苹果蠹蛾、苹果蛆和果蝇。虽然在 1970 年禁止了这些农药的广泛使用，但是砷酸铅和砷酸钙非常稳定并可长期存留于土壤之中，因此在其应用被禁止很长时间以后仍可以影响在污染土壤中生长的作物。

本文使用带高灵敏的Extractor EI 离子源的Agilent 5977A 系列GC/MSD，利用非目标化合物分析和统计工具，对化合物进行分析，以区分五种不同品牌的威士忌。

高灵敏、高准确性,Agilent针对乳粉中双氰胺检测的整体解决方案，使用高效LC MS/MS。高灵敏、高准确性,Agilent针对乳粉中双氰胺检测的整体解决方案，使用高效LC MS/MS。

Agilent 5975C GC/MS And 7000 GC/QQQ 分析食品中的塑化剂 塑化剂背景介绍 Agilent 5975C GC/MS 分析食品中的塑化剂 Agilent 7000 GC/QQQ 分析食品中的塑化剂 塑化剂分析仪器配置安捷伦邻苯二甲酸酯类化合物分析解决方案 采用液相色谱-串联质谱法测定食品添加剂中的邻苯二甲酸酯类化合物 - 非法食品添加剂- DEHP - 邻苯二甲酸酯类化合物(PAEs) - 邻苯二甲酸酯类化合物的法规认证 PAEs 分析面临那些挑战 PAEs 有那些代谢产物，这些代谢产物如何进行分析

安捷伦质谱全系列产品及分析软件，您可以通过不同特征风味物质、矿质元素多方面分析葡萄酒，从而获得准确度极高的、完整的葡萄酒检测解决方案。