贴壁细胞

贴壁细胞转基因--PP35-2P电极 是设计用于原位贴壁细胞的电穿孔或在除样品杯以外的其他容器内进行较大量的电穿孔操作，使用电极在原位对贴壁细胞进行电穿孔避免了需使用化学品消化细胞以及低穿孔效率，中断了细胞周期及细胞间的联系等诸多问题．贴壁细胞转基因--PP35-2P电极 应用于哺乳动物细胞转染，基因治疗，蛋白或药物的传递，也可应用于植物和酵母。贴壁细胞转基因--PP35-2P电极 是一种可复用的电极，适用于配装于6孔板的每个单孔或一个35mm直径的培养皿，由13个间隔2mm的镀金电极组成，适用于BTX各型电穿孔仪。The Petri Pulser? PP35-2P is a reusable electroporationapplicator designed to fit into each single well of a 6-well plateor an individual 35mm diameter petri dish. The PP35-2P consists ofan electrode assembly embedded in a polyurethane holder with highvoltage electrode cables. The thin electrodes are gold plated anddesigned to maximize the surface area of electroporation. The highvoltage cables are connected with a BTX Pulse Generator, and theelectrode head inserted into the dish or well containing thesample. An electroporation pulse may then be delivered. The entireapplicator may be cleaned with mild detergent, and the electrodesmay be sterilized with ethanol and dried with acetone.ApplicationsGeneral ApplicationsThe Petri Pulser PP35-2P is designed for the electroporation ofadherent cells in situ or as an alternative to cuvetteelectroporation for larger volumes or multiple cell samples. Theuse of the electrode for the electroporation of adherent cells insitu eliminates the need for trypsinization or mechanical removalof cells from their growth substrate. This eliminates associatedproblems including low plating efficiencies followingelectroporation, interruption of cell cycle and interruption ofintercellular communication.Mammalian Cell Protein/Drug ElectroincorporationThe Petri Pulser PP35-2P supplements our Model 366 Petri DishElectrode designed for 100 mm petri dishes, as the ideal electrodefor in situ electroincorporation of protein into mammaliancells.1,2,3 This electrode has been used for electroincorporationof peptides and proteins in CHO cells4 and vascular smooth musclecells5.Mammalian Cell Transfections/Gene TherapyThe Petri Pulser PP35-2P is capable of supporting transfectionprotocols developed for our larger Model 366 Petri Dish Electrode,including protocols for BHK and HUVEC.6 The electrode itself hasbeen used for the transfection of 3T3-L1, Rat adipose cells, SF295human neuroglioblastoma and Cos 7 cells.7Plant, Yeast and Bacterial Electroporation ApplicationsApplications involving plant, yeast and bacterial electroporationmay be carried out using the Petri Pulser. Scale up and multiplesample processing.Technical SpecificationsStandard CapabilitiesVoltage Range: 0 - 300VPulse Length Range: 1 - 35 msec in PBSVolume Range: 0.5 - 3.0 mlPhysical CharacteristicsWeight: 6 ozNumber of Electrodes: 13Electrode Thickness: 0.5 mmElectrode Material: Gold Plated.Generator Compatibility ECM 830, 630, 600, 399, 395, T 820Ordering InformationSystem Model Number Available ConfigurationsPP35-2P Petri Pulser PP35-2P and Instruction SheetReferencesMarrero et al., Journal of Biological Chemistry, 270 (26):15734-15738 (1995)Scheiffer et al., Journal of Biological Chemistry, 271 (17):10329-10333 (1996)Marrero et al., Journal of Biological Chemistry, 272 (39):24684-24690 (1997)Lobo, Errol, University of California, San Francisco, PersonalCommunication (1998)Fukai, Masuko, Emory University, Personal Communication (1998)BTX Protocols PR0165 and PR0330 (1998)BTX Protocols PR0290, PR00286, PR0359, PR0360, PR0364 (1998)

Virya™ 细胞培养皿品牌：virya™ 货号：3500356 / 3500606 / 3501006 规格：35mm / 60mm / 100mm材质：聚苯乙烯(PS)分类：细胞培养灭菌：是包装：20个/袋，25袋/箱 ?细胞培养皿是直接、方便地进行细胞处理的最佳选择。培养皿盖有叠放定位环，易于叠放，确保拿取培养皿或细胞操作时的安全。整体设计利于操作便利性和叠放稳定性，确保安全、可靠地进行细胞培养。◆高度透明、聚苯乙烯(PS)材质制成，符合USP-Class VI标准；◆真空等离子表面处理（TC处理），细胞贴壁性优良◆皿盖通气栅设计，确保气体交换；◆TC处理，无热源、无内毒素、无DNA酶、无RNA酶；◆γ射线灭菌；◆堆叠设计，易于堆放，不易滑落；◆密封包装，有效防止污染，方便易用。类别品牌货号产品名称包装规格 35mmvirya3500356细胞培养皿 35mm细胞培养皿，等离子处理20个/袋，25袋/箱60mmvirya3500606细胞培养皿 60mm细胞培养皿，等离子处理20个/袋，25袋/箱100mmvirya3501006细胞培养皿 100mm细胞培养皿，等离子处理10个/袋，30袋/箱

康宁CellCube® 细胞培养系统为贴壁细胞的大规模培养提供了一种快速、简单、紧凑的方法。它的表面是组织培养处理的生长表面，利于细胞的贴壁，并不断用新鲜培养基灌注以提高细胞生产力。CellCube系统模拟体内条件的环境，并可靠地将营养物质和氧气分布在模块内的所有细胞上，具有较低的差异梯度。CellCube模块提供传统的组织培养处理表面或Corning CellBind® 表面，用于贴壁细胞的生长。产品特点：持续灌流系统快速、简单、紧凑模拟体内环境及条件可靠的营养成分及氧气分配供给贴壁细胞规模化扩增的独立生物反应器订货信息：