肾皮质组织和腹部皮肤组织蛋白质含量的检测

高钠饮食可将大鼠肾蛋白多糖转化为促炎介质High sodium diet converts renal proteoglycans into pro-inflammatory mediators in rats

Measurements of renal and dermal sodium 

Cortical renal tissue was obtained by cutting a triangular shaped sample from the cortical area of the kidney. Abdominal skin tissue was obtained and shaved before sodium measurements. Samples were cut in half and the wet weight of each part was measured. Per sample, both halves were dried overnight at 80˚C, dry weight was measured and one of the halves solved in pure nitric acid (Sigma-Aldrich, St Louis, USA) for sodium measurements. The second half of the sample was used to calculate the amount of protein per sample by measuring the nitrogen content according to Dumas using the Gerhardt Dumatherm Nitrogen/Protein analyser (C. Gerhardt UK Ltd, Northamptonshire, UK). Sodium concentrations were measured by atomic absorption (flame) spectrometry (Thermo M Series AA Spectrometer, Thermo Fisher Scientific, Waltham, USA) and expressed per dry weight and per nitrogen content.