Agilent 6100 系列单四极杆液质联用系统

本文采用 Agilent 6100 系列单四极杆质谱，反相色谱法，建立了航空煤油中抗氧剂 2,6-二叔丁基对甲酚的检测方法，检测限为 0.35 μg/mL，线性范围为 0.35 μg/mL-50 μg/mL，保留时间 RSD 为 0.03%，峰面积 RSD 为 1.72%。

遵照 EU 2002/72 指令，应当对与食品接触的材料中可能存在的浸出物进行检测。超临界流体色谱 (SFC) 是 HPLC 的一种补充性分离技术，用于分析和检测包装材料中可能会释放出的聚合物添加剂。本文使用 Agilent 1260 Infinity 分析型 SFC 系统结合 Agilent 6130 单四极杆 LC/MS 系统，论证了 SFC 用于典型聚合物添加剂分析的适用性。

The purification of a valuable product or product library after a long and complex synthesis is a very important process. The Agilent Automated Purifi cation Software introduces an Open-Access-based solution for isolating substances, with automated focused gradient to simplify the pre- and post-purification steps. This Technical Overview describes the easy steps to follow, from crude sample analytical scouting submission to pure target product archiving or screening. For example, a purification sample containing a target peak overlapped by an impurity was purifi ed on the Agilent 1260 Infinity Preparative-Scale LC/MS Purifi cation System using the Automated Purifi cation Software. This purifi cation produced a pure target compound with 88 % recovery, saving 40 % of solvent and runtime compared to a standard purifi cation method.

Purifying a large number of compounds by liquid chromatography usually requires screening experiments and manual interaction during upscaling to preparative conditions. The Agilent Automated Purification software facilitates the workflow of analytical scouting to preparative purification by smart algorithms and calculated scale-up of separation gradients, all integrated in an intuitive interface. This Technical Overview demonstrates the purification of a compound library using the Agilent 1260 Infinity Automated LC/MSD Purification System controlled by the Automated Purification software.

治疗性多肽和蛋白质的表征及杂质分析需要联合使用色谱分离和质量选择检测。然而，多肽和蛋白质的色谱分离方法通常使用 MS 不兼容的流动相。本应用简报采用多中心切割 (MHC) 二维液相色谱 (2D-LC) 作为质量选择检测的自动脱盐工具。在第一维中，使用 MS 不兼容的流动相根据 USP 39 分析多肽胰高血糖素。然后在第二维中进行自动脱盐和质量选择检测。

稳定的 Agilent 1260 Infinity LC 与 Agilent 6120B 单四极杆质谱系统组合使用，可以对各种物理化学性质迥异的合成化合物进行可靠的分析。紫外与质谱检测的互补使用 有助于鉴定多种目标分析物。使用 Walkup 软件可以轻松创建和分析样品列，无需具备使用 LC/MS 硬件或软件的专业知识。使用 ASR 软件自动解析数据可以确保结果一致且可靠。

Reliable and easy - to - use instrumentation for compound identification as well as purity checking are required during drug discovery and development. The Agilent 6120B Single Quadrupole hardware and software are ideal to support Walkup LC/MS methods for analyzing small molecules and biomolecules in a multi - user environment. A simp lified software interface combined with robust hardware gives nonexpert users the confidence to perform high quality and reliable LC/MS analysis. Agilent MassHunter Walkup Software provides automated email reports, which allows the user to receive the r esults in an organized manner at their desk. This Application Note discusses key hardware and software features of the 6120B Single Quadrupole Walkup system, and its practical use for applications including the analysis of chemic al libraries