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当前位置: 顺元生物 > 蛋白质/多肽 > Recombinant E.Coli Dnak ATPase Binding Domain

Recombinant E.Coli Dnak ATPase Binding Domain

供货周期: 7天
品牌:
型号: 10μg
货号: HSP-010
报价: ¥700
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产品介绍
Synonyms HSP-70, HSP70, DnaK, Chaperone protein dnaK, Heat shock protein 70, Heat shock 70 kDa protein, groP, grpF, seg, b0014, JW0013.
Introduction DnaK, originally identified for its DNA replication by bacteriophage l in E. coli is the bacterial HSP-70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins.
DnaK(amino acids1-384) is N-terminal ATPase domain and ATP bound to the ATPase domain induces a conformational change in the substrate binding domain (residues 385-638). The protein coding region of the ATPase domain of DNAK (amino acids 1-384) was amplified by PCR and cloned into an E. coli expression vector. The ATPase domain of DNAK was purified to apparent homogeneity by using conventional column chromatography techniques.
Description Recombinant DnaK Substrate Binding Domain produced in E.Coli is a single, non-glycosylated polypeptide chain containing 384 amino acids and having a molecular mass of 48.1 kDa.
Source Escherichia Coli.
Physical Appearance Sterile filtered colorless solution.
Formulation The DnaK protein contains 25mM Tris-HCl, pH7.5, 100mM NaCl, 5mM DTT and 10%Glycerol.
Stability Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Sequence MGKIIGIDLG TTNSCVAIMD GTTPRVLENA EGDRTTPSII AYTQDGETLV GQPAKRQAVTNPQNTLFAIK RLIGRRFQDE EVQRDVSIMP FKIIAADNGD AWVEVKGQKM APPQISAEVLKKMKKTAEDY LGEPVTEAVI TVPAYFNDAQ RQATKDAGRI AGLEVKRIIN EPTAAALAYGLDKGTGNRTI AVYDLGGGTF DISIIEIDEV DGEKTFEVLA TNGDTHLGGE DFDSRLINYLVEEFKKDQGI DLRNDPLAMQ RLKEAAEKAK IELSSAQQTD VNLPYITADA TGPKHMNIKV TRAKLESLVE DLVNRSIEPL KVALQDAGLS VSDIDDVILV GGQTRMPMVQ KKVAEFFGKEPRKDVNPDEA VAIGAAVQGG VLTG.
Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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» DnaK SBD
» Dnak SBD C-terminus E.Coli
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上海顺元生物科技有限公司

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310116002748169

成立日期

2012-11-29

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50

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Recombinant E.Coli Dnak ATPase Binding Domain由上海顺元生物科技有限公司为您提供,货号HSP-010,规格:10μg,CAS号:,如您想了解更多关于Recombinant E.Coli Dnak ATPase Binding Domain价格、Recombinant E.Coli Dnak ATPase Binding Domain结构式、批发、用途等信息,欢迎咨询。除供应Recombinant E.Coli Dnak ATPase Binding Domain外,还可为您提供人转化生长因子β2(TGFB2)抗体ELISA kit、猴糜蛋白酶样弹性蛋白酶1(ELA1)ELISA kit、豚鼠胰淀粉酶α2A(AMY2A)ELISA Kit等试剂,公司有专业的客户服务团队,是您值得信赖的合作伙伴,顺元生物客户服务电话,售前、售后均可联系。
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