人血栓素B2ELISA试剂盒厂家
我们免费代测,品牌供应,我们拥有三千多种酶联免疫吸附测定试剂盒,包含多种种属ELISA试剂盒,待测标本形式多样:有液体及半固体形式。
样本保存:
2-8℃冰箱最多2周
-20到-50℃的冰箱可以放3-6个月
人血栓素B2ELISA试剂盒厂家-50℃一下的冰箱,可以放一年
样本收集:
血清样本用干燥管(红色)或者促凝管(黄色)收集
血浆用抗凝管,一般有EDTA抗凝(紫色)、枸橼酸钠抗凝(黑色或者蓝色)
血浆样本最好是不要用肝素(绿色)抗凝的试管收集
样本处理:
1)血清:室温血液自然凝固10-20分钟,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如出现沉淀,应再次离心。
2)血浆:应根据标本的要求选择EDTA或柠檬酸钠作为抗凝剂,混合10-20分钟后,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如有沉淀形成,应该再次离心。
3)尿液:用无菌管收集,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如有沉淀形成,应再次离心。胸腹水、脑脊液参照实行。
4)细胞培养上清:检测分泌性的成份时,用无菌管收集。离心20分钟左右(2000-3000转/分)。仔细收集上清。检测细胞内的成份时,用PBS(PH7.2-7.4)稀释细胞悬液,细胞浓度达到100万/ml左右。人血栓素B2ELISA试剂盒厂家通过反复冻融,以使细胞破坏并放出细胞内成份。离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。
5)组织标本:切割标本后,称取重量。加入一定量的PBS,PH7.4。用液氮迅速冷冻保存备用。标本融化后仍然保持2-8℃的温度。加入一定量的PBS(PH7.4),用手工或匀浆器将标本匀浆充分。离心20分钟左右(2000-3000转/分)。仔细收集上清。分装后一份待检测,其余冷冻备用。
6)标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验。若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融.
7)不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的(HRP)活性。
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EIA(B004)-029148 人无翅型MMTV整合位点家族成员11(WNT11)ELISA Kit ,Products name: Human WNT11 ELISA Kit ,灵敏度: 7.5pg/mL ,检测范围: 12.5-800pg/mL
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EIA(B004)-029150 人无翅型MMTV整合位点家族成员3(WNT3)ELISA Kit ,Products name: Human WNT3 ELISA Kit ,灵敏度: 37.5pg/mL ,检测范围: 62.5-4000pg/mL
EIA(B004)-029151 人无翅型MMTV整合位点家族成员3A(WNT3A)ELISA Kit ,Products name: Human WNT3A ELISA Kit ,灵敏度: 1.875ng/mL ,检测范围: 3.125-200ng/mL
EIA(B004)-029152 人无翅型MMTV整合位点家族成员4(WNT4)ELISA Kit ,Products name: Human WNT4 ELISA Kit ,灵敏度: 0.93799999999999994ng/mL ,检测范围: 1.5629999999999999-100ng/mL
EIA(B004)-029153 人无翅型MMTV整合位点家族成员5A(WNT5A)ELISA Kit ,Products name: Human WNT5A ELISA Kit ,灵敏度: 0.188ng/mL ,检测范围: 0.313-20ng/mL
EIA(B004)-029154 人无翅型MMTV整合位点家族成员5B(WNT5B)ELISA Kit ,Products name: Human WNT5B ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-029155 人无翅型MMTV整合位点家族成员7A(WNT7A)ELISA Kit ,Products name: Human WNT7A ELISA Kit ,灵敏度: 18.75pg/mL ,检测范围: 31.25-2000pg/mL
EIA(B004)-029156 人无翅型MMTV整合位点家族成员7B(WNT7B)ELISA Kit ,Products name: Human WNT7B ELISA Kit ,灵敏度: 0.188ng/mL ,检测范围: 0.313-20ng/mL
EIA(B004)-029157 人无翅型MMTV整合位点家族成员9B(WNT9B)ELISA Kit ,Products name: Human WNT9B ELISA Kit ,灵敏度: 0.094ng/mL ,检测范围: 0.156-10ng/mL
EIA(B004)-029158 人无花果酶2(FCN2)ELISA Kit ,Products name: Human FCN2 ELISA Kit ,灵敏度: 0.188ng/mL ,检测范围: 0.313-20ng/mL
EIA(B004)-029159 人无脉络膜蛋白(CHM)ELISA Kit ,Products name: Human CHM ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-029160 人无羊膜蛋白(AMN)ELISA Kit ,Products name: Human AMN ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-029161 人五聚素3(PTX3)ELISA Kit ,Products name: PTX3 ELISA Kit ,灵敏度: 5.625ng/mL ,检测范围: 9.375-600ng/mL
EIA(B004)-029162 人五聚素3(PTX3)ELISA Kit ,Products name: Human PTX3 ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-029163 人五聚素3(PTX3)ELISA Kit ,Products name: PTX3 ELISA Kit ,灵敏度: 0.46899999999999997ng/mL ,检测范围: 0.78100000000000003-50ng/mL
EIA(B004)-029164 人五羟色胺(5HT)ELISA Kit ,Products name: 5HT ELISA Kit ,灵敏度: 46.875pg/mL ,检测范围: 78.125-5000pg/mL
EIA(B004)-029165 人舞蹈病蛋白相互作用蛋白1(HIP1)ELISA Kit ,Products name: Human HIP1 ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-029166 人戊糖素(Pentosidine)ELISA Kit ,Products name: Human Pentosidine ELISA Kit ,灵敏度: 1.875ng/mL ,检测范围: 3.125-200ng/mL
EIA(B004)-029167 人戊糖素(Pentosidine)ELISA Kit ,Products name: Human Pentosidine ELISA Kit ,灵敏度: 0.188ng/mL ,检测范围: 0.313-20ng/mL
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EIA(B004)-029169 人戊糖素(PTD)ELISA Kit ,Products name: Human PTD ELISA Kit ,灵敏度: 0.188ng/mL ,检测范围: 0.313-20ng/mL
EIA(B004)-029170 人戊型肝炎病毒IgG(HEV-IgG)ELISA Kit ,Products name: Human hepatitis E virus IgG,HEV-IgG ELISA Kit ,灵敏度: 46.875pg/mL ,检测范围: 78.125-5000pg/mL
EIA(B004)-029171 人戊型肝炎病毒IgM(HEV-IgM)ELISA Kit ,Products name: Human Hepatitis D virus IgM,HDV IgM ELISA Kit ,灵敏度: 0.094ng/mL ,检测范围: 0.156-10ng/mL
EIA(B004)-029172 人硒代半胱氨酸裂解酶(SCLY)ELISA Kit ,Products name: Human SCLY ELISA Kit ,灵敏度: 0.094ng/mL ,检测范围: 0.156-10ng/mL
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