NFKB激活蛋白1抗体

NFKB激活蛋白1抗体 

『别名』MAP3K7IP 3; Mitogen activated protein kinase kinase kinase 7 interacting protein 3; Mitogen-activated protein kinase kinase kinase 7-interacting protein 3; NAP1; NF kappa B activating protein 1; NF-kappa-B-activating protein 1; NFkB activating protein 1; TAB-3; TAB3; TAB3_HUMAN; TAK1 binding protein 3; TAK1-binding protein 3; TGF-beta-activated kinase 1 and MAP3K7-binding protein 3; TGF-beta-activated kinase 1-binding protein 3. 

『浓度』NFKB激活蛋白1抗体1mg/1ml 

『规格』0.1ml/100μg  0.2ml/200μg     

『抗体来源』Rabbit  

抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展，抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务，并将成为您在科研及生产中的得力助手。

我公司免费提供抗血清亲合层析纯化服务，得到目的蛋白的抗体IgG。

我公司还免费提供真空冷冻抽干服务，得到冻干粉状的抗体IgG产品，以便产品的使用及长期保存。

NFKB激活蛋白1抗体洗脱问题

抗体组目前采用的是低pH洗脱方法，按道理说，这个方法应该可以洗脱掉纯大部分抗体，但是有时候出现了一些顽固抗体很难洗脱，大家可以考虑其它的洗脱方法，beads说明书上提到了五大类办法，我觉得比较可靠的方法只有两类，一是改变pH，二是改变离子浓度，另外几种办法要么会带入有机试剂，要么会带入氨基，所以不太适用。改变pH的办法除了用低pH，还可以考虑用高pH来洗脱，好像没多少人尝试过这种办法，在确实没有办法的时候推荐尝试一下。 

NFKB激活蛋白1抗体其它问题

1、看了很多抗体纯化的资料，不管是从血清中纯化多抗还是从腹水中纯化单抗，都推荐大家在上柱子之前把血清或腹水稀释2-5倍，另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了，但是稀释这一步推荐大家试一试，有文献说稀释可以提高回收率

2、封闭这一步做完后，是不是考虑可以用HCl洗一次？连在beads上的蛋白有的是蛋白间非特异性结合上去的，洗脱之前的任何步骤也许都无法洗干净它们，所以这里把它们都洗掉，以免最后都洗到抗体里去了。

3、CNBr活化的琼脂糖或葡聚糖本身的缺陷：这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白，其中包括我们要的抗体，它们都带有一定的负电，所以都会和这个部位进行非特异性结合，在洗脱前，有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的，但是它也会洗掉直接与beads非特异结合的蛋白，也包括我们要的抗体，至于这一步会丢掉多少抗体，好像没有人测过。

EL0106c 豚鼠胸腺白血病抗原(TLA)酶联免疫吸附测定试剂盒 Guinea pig TLA (Thymus Leukemia Antigen) ELISA Kit

EL1237c 豚鼠肿瘤坏死因子配体相关分子-1A(TL1A)酶联免疫吸附测定试剂盒 Guinea pig TL1A (Tumor Necrosis Factor Related Ligand 1A) ELISA Kit

EL0551c 豚鼠紧密结合蛋白1(TJP1)酶联免疫吸附测定试剂盒 Guinea pig TJP1 (Tight Junction Protein 1) ELISA Kit 

EL0520c 豚鼠基质金属蛋白酶抑制因子3(TIMP-3)酶联免疫吸附测定试剂盒 Guinea pig TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) ELISA Kit

EL0641c 豚鼠基质金属蛋白酶抑制因子2(TIMP-2)酶联免疫吸附测定试剂盒 Guinea pig TIMP-2 (Tissue Inhibitors of Metalloproteinase 2) ELISA Kit

EL0014c 豚鼠基质金属蛋白酶组织抑制因子1(TIMP-1)酶联免疫吸附测定试剂盒 Guinea pig TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit

EL1063c 豚鼠胸腺非依赖性抗原(TI-Ag)酶联免疫吸附测定试剂盒 Guinea pig TI-Ag (Thymus Independent Antigen) ELISA Kit

EL0311c 豚鼠甲状腺素受体α(THRα)酶联免疫吸附测定试剂盒 Guinea pig THRα (Thyroid Hormone Receptor Alpha) ELISA Kit

EL1155c 豚鼠酪氨酸羟化酶(TH)酶联免疫吸附测定试剂盒 Guinea pig TH (Tyrosine Hydroxylase) ELISA Kit

EL0966c 豚鼠T细胞特异性鸟苷三磷酸酶(Tgtp)酶联免疫吸附测定试剂盒 Guinea pig Tgtp (T-cell Specific Gtpase) ELISA Kit

EL0657c 豚鼠组织转谷氨酰胺酶(TGM2)酶联免疫吸附测定试剂盒 Guinea pig TGM2 (Transglutaminase 2, Tissue) ELISA Kit

EL0488c 豚鼠角质转谷氨酰胺酶(TGM1)酶联免疫吸附测定试剂盒 Guinea pig TGM1 (Transglutaminase 1, Keratinocyte) ELISA Kit

EL0356c 豚鼠转化生长因子β3(TGF-β3)酶联免疫吸附测定试剂盒 Guinea pig TGF-β3 (Transforming Growth Factor β3) ELISA Kit 

EL0963c 豚鼠转化生长因子β2(TGF-β2)酶联免疫吸附测定试剂盒 Guinea pig TGF-β2 (Transforming Growth Factor β2) ELISA Kit

EL0158c 豚鼠转化生长因子α(TGF-α)酶联免疫吸附测定试剂盒 Guinea pig TGF-α (Transforming Growth Factor α) ELISA Kit

EL0424c 豚鼠甲状腺球蛋白(TG)酶联免疫吸附测定试剂盒 Guinea pig TG (Thyroglobulin) ELISA Kit 

EL0810c 豚鼠转铁蛋白受体2(TFR2)酶联免疫吸附测定试剂盒 Guinea pig TFR2 (Transferrin Receptor 2) ELISA Kit  

EL0187c 豚鼠转铁蛋白受体(TFR/CD71)酶联免疫吸附测定试剂盒 Guinea pig TFR/CD71 (Transferrin Receptor) ELISA Kit

EL1074c 豚鼠组织因子途径抑制因子2(TFPI2)酶联免疫吸附测定试剂盒 Guinea pig TFPI2 (Tissue Factor Pathway Inhibitor 2) ELISA Kit 

EL0614c 豚鼠组织因子途径抑制因子(TFPI)酶联免疫吸附测定试剂盒 Guinea pig TFPI (Tissue Factor Pathway Inhibitor) ELISA Kit

EL0752c 豚鼠肠三叶肽因子3(TFF3)酶联免疫吸附测定试剂盒 Guinea pig TFF3 (Trefoil Factor 3, Intestinal ) ELISA Kit

EL0507c 豚鼠三叶因子2(TFF2)酶联免疫吸附测定试剂盒 Guinea pig TFF2 (Trefoil Factor 2) ELISA Kit 

EL1012c 豚鼠转移因子(TF)酶联免疫吸附测定试剂盒 Guinea pig TF (Transfer Factor) ELISA Kit

EL0272c 豚鼠组织因子(TF)酶联免疫吸附测定试剂盒 Guinea pig TF (Tissue Factor) ELISA Kit

EL1107c 豚鼠端粒酶逆转录酶(TERT)酶联免疫吸附测定试剂盒 Guinea pig TERT (Telomerase Reverse Transcriptase) ELISA Kit

EL1200c 豚鼠促甲状腺激素胚胎因子(TEF)酶联免疫吸附测定试剂盒 Guinea pig TEF (Thyrotroph Embryonic Factor) ELISA Kit 

EL0995c 豚鼠胸腺表达趋化因子(TECK/CCL25)酶联免疫吸附测定试剂盒 Guinea pig TECK/CCL25 (Thymus Expressed Chemokine) ELISA Kit 

EL0026c 豚鼠端粒酶(TE)酶联免疫吸附测定试剂盒 Guinea pig TE (Telomerase) ELISA Kit

EL0172c 豚鼠末端脱氧核糖核酸转移酶(TDT)酶联免疫吸附测定试剂盒 Guinea pig TDT (Terminal Deoxynucleotidyl Transferase) ELISA Kit

EL0611c 豚鼠畸胎瘤衍生生长因子1(TDGF1)酶联免疫吸附测定试剂盒 Guinea pig TDGF1 (Teratocarcinoma Derived Growth Factor 1) ELISA Kit 

EL0874c 豚鼠胸腺依赖性抗原(TD-Ag)酶联免疫吸附测定试剂盒 Guinea pig TD-Ag (Thymus Dependent Antigen) ELISA Kit

EL0718c 豚鼠T细胞受体(TCR)酶联免疫吸附测定试剂盒 Guinea pig TCR (Tcell Receptor) ELISA Kit

EL0007c 豚鼠末端补体复合物C5b-9(TCC C5b-9)酶联免疫吸附测定试剂盒 Guinea pig TCC C5b-9 (Terminal Complement Complex C5b-9) ELISA Kit

EL0649c 豚鼠硫氧还蛋白结合蛋白2(TBP2)酶联免疫吸附测定试剂盒 Guinea pig TBP2 (Thioredoxin Binding Protein 2) ELISA Kit 

EL0189c 豚鼠凝血酶/抗凝血酶复合物(TAT)酶联免疫吸附测定试剂盒 Guinea pig TAT (Thrombin/Antithrombin Complex) ELISA Kit

EL0981c 豚鼠胸腺激活调节趋化因子(TARC)酶联免疫吸附测定试剂盒 Guinea pig TARC (Thymus Activation Regulated Chemokine) ELISA Kit

EL0117c 豚鼠胰蛋白酶原激活肽(TAP)酶联免疫吸附测定试剂盒 Guinea pig TAP (Trypsinogen Activation Peptide) ELISA Kit

EL0093c 豚鼠抗原提呈相关转运蛋白(TAP)酶联免疫吸附测定试剂盒 Guinea pig TAP (Transporter Associated with Antigen Processing) ELISA Kit

EL1196c 豚鼠转胶蛋白(TAGLN)酶联免疫吸附测定试剂盒 Guinea pig TAGLN (Transgelin) ELISA Kit

EL1039c 豚鼠纤溶抑制因子(TAFI)酶联免疫吸附测定试剂盒 Guinea pig TAFI (Thrombin Activatable Fibrinolysis Inhibitor) ELISA Kit 

EL0991c 豚鼠TATA框结合蛋白相关因子2(TAF2)酶联免疫吸附测定试剂盒 Guinea pig TAF2 (TATA Box Binding Protein Associated Factor 2) ELISA Kit

EL0210c 豚鼠TATA框结合蛋白相关因子13(TAF13)酶联免疫吸附测定试剂盒 Guinea pig TAF13 (TATA Box Binding Protein Associated Factor 13) ELISA Kit

EL0532c 豚鼠TATA框结合蛋白相关因子1(TAF1)酶联免疫吸附测定试剂盒 Guinea pig TAF1 (TATA Box Binding Protein Associated Factor 1) ELISA Kit 

EL0636c 豚鼠速激肽受体3(TACR3)酶联免疫吸附测定试剂盒 Guinea pig TACR3 (Tachykinin Receptor 3) ELISA Kit

EL0678c 豚鼠速激肽受体2(TACR2)酶联免疫吸附测定试剂盒 Guinea pig TACR2 (Tachykinin Receptor 2) ELISA Kit

EL0760c 豚鼠甲状腺素抗体(TAb)酶联免疫吸附测定试剂盒 Guinea pig TAB (Thyroxine Antibody) ELISA Kit

EL1319c 豚鼠胸腺肽α1(Ta1)酶联免疫吸附测定试剂盒 Guinea pig Ta1 (Thymosin-α1) ELISA Kit

EL1309c 豚鼠突触素(SYP)酶联免疫吸附测定试剂盒 Guinea pig SYP (Synaptophysin) ELISA Kit

EL1183c 豚鼠突触足蛋白(SYNPO)酶联免疫吸附测定试剂盒 Guinea pig SYNPO (Synaptopodin) ELISA Kit 

EL0927c 豚鼠丝氨酸/苏氨酸激酶39(STK39)酶联免疫吸附测定试剂盒 Guinea pig STK39 (Serine/Threonine Kinase 39) ELISA Kit

EL1092c 豚鼠信号传导转录激活因子2(STAT2)酶联免疫吸附测定试剂盒 Guinea pig STAT2 (Signal Transducer and Activator of Transcription 2) ELISA Kit 

EL1057c 豚鼠信号传导转录激活因子1(STAT1)酶联免疫吸附测定试剂盒 Guinea pig STAT1 (Signal Transducer and Activator of Transcription 1) ELISA Kit 

EL0341c 豚鼠基质裂解素(ST3)酶联免疫吸附测定试剂盒 Guinea pig ST3 (Stromelysin-3)ELISA Kit

EL0975c 豚鼠同线蛋白2(ST2)酶联免疫吸附测定试剂盒 Guinea pig ST2 (Syntenin 2) ELISA Kit 

EL0789c 豚鼠精子特异性抗原2(SSFA2)酶联免疫吸附测定试剂盒 Guinea pig SSFA2 (Sperm Specific Antigen 2) ELISA Kit 

EL0526c 豚鼠信号识别颗粒(SRP9)酶联免疫吸附测定试剂盒 Guinea pig SRP9 (Signal Recognition Particle 9kDa) ELISA Kit 

EL0571c 豚鼠皮肤反应因子/炎性因子(SRF/IF)酶联免疫吸附测定试剂盒 Guinea pig SRF/IF (Skin Reactive Factor/Inflammatory Factor) ELISA Kit

EL0191c 豚鼠胆固醇调节元件结合蛋白(SREBP)酶联免疫吸附测定试剂盒 Guinea pig SREBP (Sterol Regulatory Element Binding Proteins) ELISA Kit

EL0396c 豚鼠非红细胞血影蛋白β4 (SPTβN4)酶联免疫吸附测定试剂盒 Guinea pig SPTβN4 (Spectrin Beta, Non Erythrocytic 4) ELISA Kit

EL0422c 豚鼠斑型POZ蛋白NFKB激活蛋白1抗体(SPOP)酶联免疫吸附测定试剂盒 Guinea pig SPOP (Speckle Type POZ Protein) ELISA Kit 

EL0329c 豚鼠表面活性物质关联蛋白J(SPJ)酶联免疫吸附测定试剂盒 Guinea pig SPJ (Surfactant Associated Protein J) ELISA Kit

EL0971c 豚鼠肺表面活性物质相关蛋白D(SPD)酶联免疫吸附测定试剂盒 Guinea pig SPD (Pulmonary Surfactant Associated Protein D) ELISA Kit