磷酸化丝裂原活化蛋白激酶激酶5抗体
『别名』MAP2K5 (phospho S142); Dual specificity mitogen activated protein kinase kinase 5; MAP kinase kinase 5; MAP kinase kinase MEK5b; MAP2K5; MAPK/ERK kinase 5; MAPKK5; PRKMK5; EC 2.7.12.2; MP2K5_HUMAN; Dual specificity mitogen-activated protein kinase kinase 5; MAPKK 5; MAPK/ERK kinase 5; MEK 5.
『浓度』磷酸化丝裂原活化蛋白激酶激酶5抗体1mg/1ml
『规格』0.1ml/100μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
磷酸化丝裂原活化蛋白激酶激酶5抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
磷酸化丝裂原活化蛋白激酶激酶5抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
EL0149c 豚鼠黑色素瘤标记物(MART/Melan-A)酶联免疫吸附测定试剂盒 Guinea pig MART/Melan-A (Melanoma Marker A) ELISA Kit
EL0833c 豚鼠微管关联蛋白(MAPτ)酶联免疫吸附测定试剂盒 Guinea pig MAPτ (Microtubule Associated Protein Tau/Tau Protein) ELISA Kit
EL0140c 豚鼠丝裂原活化蛋白激酶14(MAPK14)酶联免疫吸附测定试剂盒 Guinea pig MAPK14 (Mitogen Activated Protein Kinase 14) ELISA Kit
EL0406c 豚鼠丝裂原激活蛋白激酶(MAPK)酶联免疫吸附测定试剂盒 Guinea pig MAPK (Mitogen Activated Protein Kinase) ELISA Kit
EL0860c 豚鼠丝裂原激活蛋白激酶激酶6(MAP2K6/MKK6)酶联免疫吸附测定试剂盒 Guinea pig MAP2K6/MKK6 (Mitogen Activated Protein Kinase Kinase 6) ELISA Kit
EL0558c 豚鼠单胺氧化酶(MAO)酶联免疫吸附测定试剂盒 Guinea pig MAO (Monoamine Oxidase) ELISA Kit
EL0848c 豚鼠中脑星型胶质细胞来源神经营养因子(MANF)酶联免疫吸附测定试剂盒 Guinea pig MANF (Mesencephalic Astrocyte Derived Neurotrophic Factor) ELISA Kit
EL0924c 豚鼠甘露糖苷酶α1A类成员1(MAN1A1)酶联免疫吸附测定试剂盒 Guinea pig MAN1A1 (Mannosidase Alpha Class 1A Member 1) ELISA Kit
EL0364c 豚鼠黑色素瘤相关抗原(MAGE)酶联免疫吸附测定试剂盒 Guinea pig MAGE (Melanoma-associated Antigen) ELISA Kit
EL0728c 豚鼠抗髓鞘相关糖蛋白抗体(MAG Ab)酶联免疫吸附测定试剂盒 Guinea pig MAG Ab (Anti-Myelin Associated Glycoprotein Antibody) ELISA Kit
EL0986c 豚鼠髓鞘相关糖蛋白(MAG)酶联免疫吸附测定试剂盒 Guinea pig MAG (Myelin Associated Glycoprotein) ELISA Kit
EL1189c 豚鼠毒蕈碱型乙酰胆碱受体亚型M2(M-AChRM2)酶联免疫吸附测定试剂盒 Guinea pig M-AChR M2 (Muscarinic Acetylcholine Receptor M2) ELISA Kit
EL0139c 豚鼠毒蕈碱型乙酰胆碱受体(M-AChR)酶联免疫吸附测定试剂盒 Guinea pig M-AChR (Muscarinic Acetylcholine Receptor) ELISA Kit
EL0624c 豚鼠溶菌酶(LZM)酶联免疫吸附测定试剂盒 Guinea pig LZM (Lysozyme) ELISA Kit
EL0988c 豚鼠淋巴细胞抗原6复合物基因座A(Ly6a)酶联免疫吸附测定试剂盒 Guinea pig Ly6a (Lymphocyte Antigen 6 Complex Locus A) ELISA Kit
EL1045c 豚鼠基膜聚糖(LUM)酶联免疫吸附测定试剂盒 Guinea pig LUM (Lumican) ELISA Kit
EL0713c 豚鼠白三烯E4(LTE4)酶联免疫吸附测定试剂盒 Guinea pig LT-E4 (Leukotriene E4) ELISA Kit
EL0594c 豚鼠白三烯C4(LTC4)酶联免疫吸附测定试剂盒 Guinea pig LTC4 (Leukotriene C4) ELISA Kit
EL0363c 豚鼠白三烯A4水解酶(LTA4H)酶联免疫吸附测定试剂盒 Guinea pig LTA4H (Leukotriene A4 Hydrolase) ELISA Kit
EL1211c 豚鼠白细胞调节素(LR)酶联免疫吸附测定试剂盒 Guinea pig LR (Leukoregulin) ELISA Kit
EL0286c 豚鼠淋巴细胞趋化因子(Lptn/XCL1)酶联免疫吸附测定试剂盒 Guinea pig Lptn/XCL1 (Lymphotactin) ELISA Kit
EL0116c 豚鼠脂蛋白关联磷脂酶A2(LpPLA2)酶联免疫吸附测定试剂盒 Guinea pig LpPLA2 (Phospholipase A2, Lipoprotein Associated) ELISA Kit
EL0557c 豚鼠脂蛋白a(LP-a)酶联免疫吸附测定试剂盒 Guinea pig LP-a (Lipoprotein a) ELISA Kit
EL0621c 豚鼠赖氨酰氧化酶样蛋白4(LOXL4)酶联免疫吸附测定试剂盒 Guinea pig LOXL4 (Lysyl Oxidase Like Protein 4 ) ELISA Kit
EL0416c 豚鼠凝集素样氧化低密度脂蛋白受体1(LOX-1)酶联免疫吸附测定试剂盒 Guinea pig LOX-1 (Lectin Like Oxidized Low Density Lipoprotein Receptor 1) ELISA Kit
EL0509c 豚鼠层粘蛋白(LN)酶联免疫吸附测定试剂盒 Guinea pig LN (Laminin) ELISA Kit
EL1285c 豚鼠L-乳酸脱氢酶(L-LDH)酶联免疫吸附测定试剂盒 Guinea pig L-LDH (L-Lactate Dehydrogenase) ELISA Kit
EL0839c 豚鼠脂肪酶H (LIPH)酶联免疫吸附测定试剂盒 Guinea pig LIPH (Lipase H) ELISA Kit
EL0347c 豚鼠肝脂酶 (LIPC)酶联免疫吸附测定试剂盒 Guinea pig LIPC (Lipase, Hepatic) ELISA Kit
EL0623c 豚鼠白细胞免疫球蛋白样受体亚家族B成员4(LILRB4)酶联免疫吸附测定试剂盒 Guinea pig LILRB4 (Leukocyte Immunoglobulin Like Receptor Subfamily B Member 4) ELISA Kit
EL0464c 豚鼠白血病抑制因子受体(LIFR)酶联免疫吸附测定试剂盒 Guinea pig LIFR (Leukemia Inhibitory Factor Receptor) ELISA Kit
EL0177c 豚鼠白血病抑制因子(LIF)酶联免疫吸附测定试剂盒 Guinea pig LIF (Leukemia Inhibitory Factor) ELISA Kit
EL0169c 豚鼠促黄体生成激素(LH)酶联免疫吸附测定试剂盒 Guinea pig LH (Luteinizing Hormone) ELISA Kit
EL0445c 豚鼠淋巴细胞功能相关抗原3(LFA-3/CD58)酶联免疫吸附测定试剂盒 Guinea pig LFA-3 (Lymphocyte Function Associated Antigen 3) ELISA Kit
EL0103c 豚鼠淋巴细胞功能相关抗原2(LFA-2/CD2)酶联免疫吸附测定试剂盒 Guinea pig LFA-2/CD2 (Lymphocyte Function Associated Antigen 2) ELISA Kit
EL0545c 豚鼠瘦素受体(LEPR)酶联免疫吸附测定试剂盒 Guinea pig LEPR ( Leptin Receptor) ELISA Kit
EL0762c 豚鼠瘦素(LEP)酶联免疫吸附测定试剂盒 Guinea pig LEP (Leptin) ELISA Kit
EL0394c 豚鼠低密度脂蛋白(LDL)酶联免疫吸附测定试剂盒 Guinea pig LDL (Low Density Lipoprotein) ELISA Kit
EL0133c 豚鼠乳酸脱氢酶C(LDHC)酶联免疫吸附测定试剂盒 Guinea pig LDHC (Lactate Dehydrogenase C) ELISA Kit
EL0474c 豚鼠乳酸脱氢酶B(LDHB)酶联免疫吸附测定试剂盒 Guinea pig LDHB (Lactate Dehydrogenase B) ELISA Kit
EL0359c 豚鼠脂质运载蛋白1(LCN1)酶联免疫吸附测定试剂盒 Guinea pig LCN1 (Lipocalin 1) ELISA Kit
EL0759c 豚鼠脂多糖结合蛋白(LBP)酶联免疫吸附测定试剂盒 Guinea pig LBP (Lipopolysaccharide Binding Protein) ELISA Kit
EL1218c 豚鼠长效甲状腺刺激素(LATS)酶联免疫吸附测定试剂盒 Guinea pig LATS (Long Acting Thyroid Stimulator) ELISA Kit
EL0843c 豚鼠T细胞活化连接蛋白(LAT)酶联免疫吸附测定试剂盒 Guinea pig LAT (Linker For Activation of T-cell) ELISA Kit
EL0895c 豚鼠亮氨酰氨基肽酶(LAP)酶联免疫吸附测定试剂盒 Guinea pig LAP (Leucine Aminopeptidase) ELISA Kit
EL1304c 豚鼠激肽释放酶6(KLK6)酶联免疫吸附测定试剂盒 Guinea pig KLK6 (Kallikrein 6) ELISA Kit
EL0291c 豚鼠角化细胞内分泌因子(KAF)酶联免疫吸附测定试剂盒 Guinea pig KAF (Keratinocyte Autocrine Factor) ELISA Kit
EL0152c 豚鼠内凝集蛋白1(ITLN1)酶联免疫吸附测定试剂盒 Guinea pig ITLN1 (Intelectin 1/Omentin) ELISA Kit
EL1234c 豚鼠干扰素诱导T细胞α亚族趋化因子 (I-TAC)酶联免疫吸附测定试剂盒 Guinea pig I-TAC (Interferon Inducible T-cell Alpha Chemoattractant) ELISA Kit
EL0038c 豚鼠钙非依赖性磷脂酶A2(iPLA2)酶联免疫吸附测定试剂盒 Guinea pig iPLA2 (Phospholipidase A2, Calcium Independent) ELISA Kit
EL0697c 豚鼠胰岛素受体(INSR)酶联免疫吸附测定试剂盒 Guinea pig INSR (Insulin Receptor) ELISA Kit
EL1181c 豚鼠胰岛素样蛋白3(INSL3)酶联免疫吸附测定试剂盒 Guinea pig INSL3 (Insulin Like Protein 3) ELISA Kit
EL1356c 豚鼠胰岛素(INS)酶联免疫吸附测定试剂盒 Guinea pig INS (Insulin) ELISA Kit
EL1291c 豚鼠白介素9磷酸化丝裂原活化蛋白激酶激酶5抗体(IL-9)酶联免疫吸附测定试剂盒 Guinea pig IL-9 (Interleukin 9) ELISA Kit
EL0879c 豚鼠白介素6受体(IL-6R)酶联免疫吸附测定试剂盒 Guinea pig IL-6R (Interleukin 6 Receptor) ELISA Kit
EL0045c 豚鼠白介素6(IL-6)酶联免疫吸附测定试剂盒 Guinea pig IL-6(Interleukin 6)ELISA Kit
EL0043c 豚鼠白介素4(IL-4)酶联免疫吸附测定试剂盒 Guinea pig IL-4 (Interleukin 4) ELISA Kit