肠道菌增菌肉汤(EE)Enterobacteria Enrichment Broth250g用于肠道菌的增菌培养(GB、FDA标准)
肠道菌增菌肉汤(EE)
Enterobacteria Enrichment Broth
用途:用于肠道菌的增菌培养(GB标准)
肠道菌增菌肉汤(EE)配方:(g/L)
蛋白胨 10.0
葡萄糖 5.0
磷酸氢二钠 8.0
KH2PO4 2.0
牛胆盐 20.0
煌绿 0.015
pH值7.2 ± 0.2 25℃
原理:
蛋白胨提供蛋白质、维生素和氨基酸;葡萄糖提供碳源;磷酸氢二钠和KH2PO4为缓冲剂;牛胆盐和煌绿为选择性抑菌剂,抑制非肠杆菌科细菌的生长。
肠道菌增菌肉汤(EE)用法:
称取本品 45.0g,加热搅拌溶解于 1000ml 蒸馏水中,分装三角瓶每瓶 90ml, 100℃水浴加热 30 分钟 , 迅速在流动的冷水中冷却,无需高压灭菌
质量控制:
在36±1℃培养18-24小时。
质控菌株菌株编号生长情况其它特征
金黄色葡萄球菌ATCC25923- /
大肠埃希氏菌ATCC25922+++ /
阴沟肠杆菌ATCC23355+++ /
沙门氏菌ATCC14028+++ /
Med(01)001627卵黄琼脂培养基基础,规格:250g/瓶,英文名:Egg Yolk Agar Base,用途:用于梭菌的分离培养,每100ml添加2支S3405
Med(01)001628胰胨-亚硫酸盐-环丝氨酸(TSC)琼脂基础/亚硫酸盐-环丝氨酸(SC)琼脂基础,规格:250g/瓶,英文名:Tryptose Sulfite Cycloserine Agar Base,用途:用于产气荚膜梭菌计数,每250ml添加1支S5602和5支S3405
Med(01)001629胰胨-亚硫酸盐-环丝氨酸(TSC)琼脂基础/亚硫酸盐-环丝氨酸(SC)琼脂基础,规格:250g/瓶,英文名:Tryptose Sulfite Cycloserine Agar Base,用途:用于产气荚膜梭菌计数,每250ml添加1支S5602和5支S3405
Med(01)001630SC琼脂基础,规格:250g/瓶,英文名:Sulfite Cycloserine Agar Base,用途:用于产气荚膜梭菌的计数和培养,需添加S5602及S3405
Med(01)001631厌氧卵黄琼脂基础,规格:250g/瓶,英文名:Anaerobic Egg Yolk Agar Base,用途:用于肉毒梭菌增菌分离,每100ml培养基中添加3支S3405
Med(01)001632CCFA琼脂基础,规格:250g/瓶,英文名:Cycloserine Cefoxitin Fructose Agar Base,用途:用于艰难梭菌的选择性分离.每200ml添加1支S5602、2支S3405、1支S5607
Med(01)001633梭杆菌选择性琼脂基础,规格:250g/瓶,英文名:Fusobacterium Selective Agar (FSA) Base,用途:用于梭杆菌的选择性分离和培养,需添加羊血及抗生素
Med(01)001634肝汤琼脂,规格:250g/瓶,英文名:Liver Broth Agar,用途:用于厌氧菌检验
Med(01)001635亚硫酸盐-多粘菌素-磺胺嘧啶(SPS)琼脂基础,规格:250g/瓶,英文名:Sulfite-Polymyxin-Sulphadiazine Agar Base,用途:用于产气荚膜梭菌计数,每100ml添加1支S5603
Med(01)001636含铁牛奶培养基(含铁牛乳培养基),规格:250g/瓶,英文名:Iron Milk Medium,用途:用于产气荚膜梭菌暴烈发酵试验
Med(01)001637胰酪蛋白胨葡萄糖酵母浸粉牛肉浸粉培养基(TPGYB),规格:250g/瓶,英文名:Trypticase peptone Glucose Yeast Extract Beef,用途:用于肉毒梭菌增菌培养
Med(01)001638产芽孢肉汤,规格:250g/瓶,英文名:Sporulation Broth,用途:用于产气荚膜梭菌的芽孢试验
Med(01)001639多价蛋白胨-酵母膏(PY)培养基,规格:250g/瓶,英文名:Poly-Peptone Extract Medium,用途:用于产气荚膜梭菌培养
Med(01)001640缓冲动力-硝酸盐培养基基础,规格:250g/瓶,英文名:Buffered Power-Nitrate Medium Base,用途:用于产气荚膜梭菌的硝酸盐还原试验,每200ml添加1支S0502
Med(01)001641乳糖-明胶培养基,规格:250g/瓶,英文名:Lactose-Gelatin Medium,用途:用于产气荚膜梭菌的乳糖、明胶生化试验
Med(01)001642亚硫酸铁琼脂,规格:250g/瓶,英文名:Sulfite Iron Agar,用途:用于厌氧亚硫酸盐还原梭状芽胞杆菌的平板计数
Med(01)001643胰蛋白胨葡萄糖酵母浸膏肉汤(TPGYT基础),规格:250g/瓶,英文名:TPGY Broth(TPGYT Base),用途:用于肉毒梭菌增菌培养,添加1:250胰蛋白酶配制成TPGYT
Med(01)001644明胶磷酸盐缓冲液,规格:250g/瓶,英文名:Gelatin PBS Broth,用途:用于肉毒梭菌和肉毒毒素检样的制备
Med(01)001645TSN琼脂,规格:250g/瓶,英文名:Trypticase Sulfite Neomycin Agar,用途:用于产气荚膜梭菌的选择性分离和计数
Med(01)001646TBB培养基,规格:250g/瓶,英文名:Tyrobutyricun Broth Base,用途:用于乳酪产品中梭菌的计数
Med(01)001647强化梭菌鉴别琼脂(DRCA),规格:250g/瓶,英文名:Differentia Reinforced Clostridial Agar,用途:用于食品和其它物质中梭菌的计数和培养
Med(01)001648梭菌鉴别琼脂(DCA),规格:250g/瓶,英文名:Differentia Clostridial Agar,用途:用于干燥食品中亚硫酸盐还原梭菌的计数
Med(01)001649强化梭菌培养基(RCM),规格:250g/瓶,英文名:Reinforced Clostridium Medium,用途:用于梭菌的培养
Med(01)001650强化梭菌琼脂,规格:250g/瓶,英文名:Reinforced Clostridium Agar,用途:用于梭菌的分离培养
Med(01)001651艰难梭菌选择性琼脂基础(CDA),规格:250g/瓶,英文名:Clostridium difficile Selective Agar,用途:用于艰难梭菌的选择性分离.每400ml添加1支S5602、1支S5607、3支S3405、4ml马血
Med(01)001652梭状芽孢杆菌计数琼脂,规格:250g/瓶,英文名:Clostridia Count Agar,用途:用于厌氧亚硫酸盐梭状芽孢杆菌计数
Med(01)001653庖肉培养基基础,规格:250g/瓶,英文名:Cooked Meat Medium Base,用途:含9.8%牛心浸粉,用于厌氧菌的培养,需添加适量S5604
Med(01)001654改良FM琼脂,规格:250g/瓶,英文名:FM Agar, Modified,用途:用于梭杆菌的分离和培养
Med(01)001655TYA培养基基础,规格:250g/瓶,英文名:TYA Medium Base,用途:用于厌氧菌的培养,每升培养基中需添加3g醋酸铵
Med(01)001656硫乙醇酸盐培养基(Brewer),规格:250g/瓶,英文名:Thioglycollate Medium (Brewer),用途:以美蓝/亚甲基蓝为指示剂,用于厌氧菌培养
Med(01)001657碎肉肉汤基础,规格:250g/瓶,英文名:Chopped Meat Broths(CM),用途:用于厌氧菌特别是专性厌氧菌的培养,含VK ,需添加S5604
Med(01)001658碎肉葡萄糖肉汤基础,规格:250g/瓶,英文名:Chopped Meat Glucose(CMG),用途:用于厌氧菌特别是专性厌氧菌的培养,含VK ,需添加S5604
Med(01)001659碎肉碳水化合物肉汤基础,规格:250g/瓶,英文名:Chopped Meat Carbohydrate Broth(CMC),用途:用于厌氧菌特别是专性厌氧菌的培养,含VK ,需添加S5604
Med(01)001660厌氧基础肉汤,规格:250g/瓶,英文名:Anaerobe Basal Broth,用途:用于厌氧菌尤其是拟杆菌的培养
Med(01)001661厌氧基础琼脂,规格:250g/瓶,英文名:Anaerobe Basal Agar,用途:用于厌氧菌尤其是拟杆菌的培养,需添加5-10%无菌脱纤维绵羊全血
Med(01)001662葡萄糖血琼脂基础(新霉素葡萄糖血琼脂基础),规格:250g/瓶,英文名:Glucose Blood Agar,用途:用于细菌的培养,需添加5-10%脱纤维绵羊血。每100ml添加1支S4105制备新霉素葡萄糖血琼脂
Med(01)001663葡萄糖血琼脂基础(新霉素葡萄糖血琼脂基础),规格:250g/瓶,英文名:Glucose Blood Agar,用途:用于细菌的培养,需添加5-10%脱纤维绵羊血。每100ml添加1支S4105制备新霉素葡萄糖血琼脂
Med(01)001664CDC厌氧菌琼脂基础,规格:250g/瓶,英文名:CDC Anaerobe Agar Base,用途:用于厌氧菌的分离培养,每100ml添加S5605和S5606各1支,添加5-10%羊血(S0002)
Med(01)001665AN厌氧琼脂基础,规格:250g/瓶,英文名:Anaerobic Agar Base,用途:厌氧基础培养基,每100ml添加S5605和S5606各1支
Med(01)001666AN厌氧肉汤基础,规格:250g/瓶,英文名:Anaerobic Broth Base,用途:厌氧基础培养基,每100ml添加S5605和S5606各1支
Med(01)001667拟杆菌胆汁七叶苷琼脂基础(BBE),规格:250g/瓶,英文名:Bacteroides Bile Esculin Agar Base,用途:用于脆弱拟杆菌的分离,每100ml添加2支S5605和5支S5601
Med(01)001668BDS培养基,规格:250g/瓶,英文名:Bacteroid Selective Agar,用途:用于拟杆菌的选择性分离
Med(01)001669GAM肉汤,规格:250g/瓶,英文名:Glfu Anaerobic Medium Broth,用途:用于厌氧菌的培养
Med(01)001670GAM半固体培养基,规格:250g/瓶,英文名:Glfu Anaerobic Medium Semisolid,用途:用于厌氧菌的保存
Med(01)001671GAM半固体培养基(不含葡萄糖),规格:250g/瓶,英文名:Glfu Anaerobic Medium Semisolid without Dextrose,用途:用于厌氧菌的保存
Med(01)001672GAM琼脂,规格:250g/瓶,英文名:Glfu Anaerobic Medium Agar,用途:用于厌氧菌的培养
Med(01)001673改良GAM肉汤基础,规格:250g/瓶,英文名:Glfu Anaerobic Medium Broth Base,Modified,用途:用于厌氧菌的培养,每100ml添加S5605、S5606各1支
Med(01)001674改良GAM琼脂基础,规格:250g/瓶,英文名:Glfu Anaerobic Medium Agar Base,Modified,用途:用于厌氧菌的培养,每100ml添加S5605、S5606各1支
Med(01)001675Wilkins-Chalgren厌氧菌琼脂,规格:250g/瓶,英文名:Wilkins-Chalgren Anaerobe Agar,用途:用于厌氧菌的培养和药敏试验
Med(01)001676Wilkins-Chalgren厌氧菌肉汤,规格:250g/瓶,英文名:Wilkins-Chalgren Anaerobe Broth,用途:肠道菌增菌肉汤(EE)用于厌氧菌的培养和药敏试验
Med(01)001677厌氧菌肉汤MIC,规格:250g/瓶,英文名:Anaerobe Broth MIC,用途:用于肉汤微量稀释法测定厌氧菌敏感性养和厌氧菌培养