效率的因素主要有5个方面：①抗体对抗原的亲合性；②抗体对抗原的特异性；③抗原表位结构在该技术中是如何被改变的；④抗体与抗原结合的易接近性；⑤二级试剂的质量和类型
英文名称 Anti-ERp19
中文名称 内质网蛋白ERp19抗体
别 名 AG1; AGR1; anterior gradient homolog 1; endoplasmic reticulum protein ERp19; endoplasmic reticulum resident protein 18; endoplasmic reticulum resident protein 19; endoplasmic reticulum thioredoxin superfamily member, 18 kDa; ER protein 18; ER protein 19; ERP 18; ERP16; ERP19; hAG 1; hAG1; hTLP19; PDIA16; protein disulfide isomerase family A, member 16; thioredoxin domain containing 12 (endoplasmic reticulum; Thioredoxin domain-containing protein 12; thioredoxin like protein p19; TLP19; TXNDC12; TXD12_HUMAN.
内质网蛋白ERp19抗体浓 度 1mg/1ml
规 格 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Sheep
产品类型 一抗
研究领域 细胞生物 信号转导 转运蛋白
蛋白分子量 predicted molecular weight: 17kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human ERp19
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 Endoplasmic reticulum proteins (ERps) are widely expressed proteins and localize to the ER. ERp19, ERp29, ERp46, ERp57 and ERp72 may act as proteases, protein disulfide isomerases, thiol-disulfide oxidases, phospholipases or a combination of these. ERp19, also designated thioredoxin domain-containing protein 12 (TXNDC12), and ERp46, also designated thioredoxin domain containing 5 (TXNDC5), belong to the thioredoxin superfamily and contain a thioredoxin fold with a consensus active-site sequence (CxxC). Both ERp19 and ERp46 are widely expressed ER luminal proteins that are most abundant in the liver and are enriched in purified liver ER vesicles. ERp19 shows significant protein thiol-disulfide oxidase activity in vitro, which is dependent on the presence of both active-site cysteines.
Function : ERp19 belongs to the thioredoxin superfamily. Members of this superfamily possess a thioredoxin fold with a consensus active-site sequence (CxxC) and have roles in redox regulation, defense against oxidative stress, refolding of disulfide-containing proteins, and regulation of transcription factors. Possesses significant protein thiol-disulfide oxidase activity.
Subcellular Location : Endoplasmic reticulum.
Tissue Specificity : Widely expressed.
Similarity : Contains 1 thioredoxin domain.
Database links : UniProtKB/Swiss-Prot: O95881.1
半个多世纪以来，抗体一直作为常用的研究工具，因此对其使用和储存已有丰富的经验。这些经验让人们知道如何对抗体进行操作，由于抗体的稳定性好，使用并不困难。抗体较其他蛋白不易变性，易于存放、纯化和标记。蛋白G和蛋白A等抗体结合蛋白的发现使得这项工作更具可行性。
选择合适的抗体对免疫化学技术的成功极为重要。没有任何一种来源的抗体能够满足本书所述的各种方法的要求。为了研究某一重要的抗原，应选用具有不同特性的多种抗体。在没有一套良好特性的抗体可用于研究机体中每一种蛋白之前，选择及制备适合各种技术要求的抗体非常必要。
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更多内质网蛋白ERp19抗体价格、批发价、品牌、厂家及抗体详细说明书，都可以免费咨询及索取。
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