药物开发 ∣ 斑马鱼等模式生物的高通量筛选方法介绍—Molecular Devices

基于斑马鱼的体内实验应用有大量的已开发的人类疾病斑马鱼模型如：
1.代谢综合症：肥胖 (内脏肥胖)、 血脂异常、脂肪肝、糖耐受失调
2.人类癌症细胞异种移植：肿瘤血管生成、远端转移
3.循环疾病：心力衰竭、药物诱导的心率失调
4.中央神经系统失调：耳聋、视力障碍、嗅觉失调、癫痫、发育紊乱、睡眠唤醒障碍、肌营养不良 (ALS)

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2014 Jan 15; 9(1).
2. Umemoto, N., et al., Fluorescent-based methods for gene knockdown and

functional cardiac imaging in zebrafish, Mol Biotechnol, 2013 Oct; 55(2): 131-

42.
3. Kanungo, J., et al., In vivo imaging and quantitative analysis of changes in

axon length using transgenic zebrafish embryos. Neurotoxicol Teratol, 2011

Nov-Dec; 33(6): 618-23.
4. Diekmann, H., et al., Characterization of optic nerve regeneration using

transgenic zebrafish. Front Cell Neurosci, 2015 April 9; 9:118.
5. Huan, H., et al., High-throughput screening for bioactive molecules using

primary cell culture of transgenic zebrafish embryos. Cell Rep, 2012 Sep 27;

2(3):695-704.

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