Mouse Anti Human Vimentin Biotin

Vimentin Antibody, Biotin
Vimentin, Mouse Anti Human Antibody, Biotin
Catalog No. ANT-246

Type:
Mouse Anti Human Monoclonal Antibody

Introduction:
Vimentin expression in human malignant glioma cells depends on cellular density, algorithms of drug delivery and chemo/radio treatment. Vimentin and detyrosinated microtubules provide structural support for the extensive microtentacles observed in detached tumor cells and a mechanism to promote successful metastatic spread. Primary colorectal carcinomas display aberrant expression of vimentin, and have activated Notch and TGFbeta signaling pathways. Vimentin is a strong arterial substrate for transglutaminases. Transglutaminase-mediated vimentin dimerization results in a novel unifying pathway by which vasodilatory and remodeling responses may be regulated. Ablation of vimentin expression inhibits migration and invasion of colon and breast cancer cell lines.

Clone:
hVimentin

Immunogen:
Purified Human Vimentin

Ig Subclass:
mouse IgG1

Purification Method:
Ion exchange column

Protein concentration:
1mg/ml in PBS (after reconstitution).

Shipping Conditions:
Antibody is shipped lyophilized at ambient temperature.

Storage Procedures:
In lyophilized form, for long periods, store at 4°C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20°C.

Reconstitution:
Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Stability / Shelf Life:
Two years lyophilized, one month in solution at 4°C.

Titer:
For intra-cellular staining, use 5-10µl/106 cells. Wash twice with PBS/Azide before adding the appropriate amount of secondary fluorescent reagent.

Cross Reactivity:
Vimentin antibody will recognize most mammalian Vimentin except mouse and rat.

Applications:
Immunohistochemistry.

Other Available Forms:
This antibody is also available conjugated to FITC and non conjugate form.

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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