Recombinant Human C-SRC

C-SRC Human
C-SRC/ CSK Human Recombinant
Catalog No. PKA-317

Synonyms:
Tyrosine-protein kinase CSK, EC 2.7.10.2, C-SRC kinase, Protein-tyrosine kinase CYL, CSK, C-SRC.

Introduction:
SRC family kinases are involved in transducing growth factor signals for cellular differentiation and proliferation in a variety of cell types. The activity of all Src family kinases (SFKs) is controlled by phosphorylation at their C-terminal 527-tyrosine residue by C-terminal SRC kinase, CSK. The C-terminal SRC kinase (CSK) family of protein tyrosine kinases contains two members: CSK and CSK homologous kinase (CHK). Both phosphorylate and inactivate SRC family kinases.
C-terminal SRC kinase (CSK) contains a catalytic domain and a regulatory region, consisting of an SH3 and an SH2 domain.

Description:
CSRC Human Recombinant produced in Sf9 cells is a glycosylated, polypeptide chain having a molecular mass of 60 KD.
CSRC is purified by proprietary chromatographic techniques.

Source:
Sf9 Insect Cells

Appearance:
Sterile Filtered clear solution.

Formulation:
C-SRC is supplied at a concentration of 0.29mg/ml in 50mM Tris, pH 7.6, 0.15 M NaCl, 0.27M sucrose, 1mM DTT, 0.1mM EGTA, 0.2mM PMSF, 1mM benzamidine, 5mM NaF, 10mM β-glycerol phosphate, 0.1mM sodium vanadate and 0.03% Brij-35.

Biological Activity:
750 units/mg. One unit of C-SRC activity is equal to 1 nmol of phosphate transferred to a peptide corresponding to p34cdc2 (6-20) per minute at 30°C with a final ATP concentration of 100 µM.
Recombinant human active C-SRC also phosphorylates STAT, p34cdc2, gastrin, and cortactin.
Please note:Kinase activity may vary depending on the substrate and reaction conditions.

Storage Conditions:
Store at 4°C if entire vial will be used within 1-2 weeks. Store frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

Purity:
Greater than 95% as determined by SDS-PAGE.

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.