聚类分析辅助中药寡糖电泳分析鉴定中药

2015-03-27 15:27

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摘要：基于中药多糖结构的复杂性和特征性，针对多糖部分降解后的寡糖片段，建立r一种采用毛细管区带电泳法 (czE)分离分析中药寡糖，并利用其特征性电泳谱图信息，结合聚类分析(cA)进行中药鉴定的方法。该方法以1．苯基一3一甲基一5一吡唑啉酮(PMP)为寡糖柱前衍生化试剂，对3个科属的6种中药如黄精、玉竹等同时进行鉴定。采用的电泳条件：未涂层熔融石英毛细管柱(49 cm(有效氏度40 cm)×50¨m)，以50 mmoL／L磷酸盐缓冲液(pH 2．5)为运行缓冲液，检测波长为245 nm，运行电压为15 kV，虹吸进样10 cm×4 s，柱温为室温。结果表明聚类分析辅助中药寡糖电泳分析法可有效用于3个科属6种中药的鉴定。本方法结果可靠，重现性好，可以作为中药鉴定的一种有效手段。

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改进的PMP 衍生方法测定天门冬多糖的单糖组成

Study on the separation mechanism of 1-phenyl-3-methyl-5-pyrazolone derivatives of aldoses in acid buffer by capillary zone electrophoresis

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