In Situ Synthesis of MIL-100(Fe) in the Capillary Column for Capillary Electrochromatographic Separation of Small Organic Molecules
Because of the unusual properties of the structure, the
metal organic frameworks (MOFs) have received great interest in
separation science. However, the most existing methods for the
applications of MOFs in separation science require an off-line
procedure to prepare the materials. Here, we report an in situ, layerby-
layer self-assembly approach to fabricate MIL-100(Fe) coated
open tubular (OT) capillary columns for capillary electrochromatography.
By a controllable manner, the OT capillary columns with a
tailored MIL-100(Fe) coating have been successfully synthesized.
The results of SEM, XRD, FT-IR, and ICP-AES indicated that MIL-
100(Fe) was successfully grafted on the inner wall of the capillary.
Some neutral, acidic and basic analytes were used to evaluate the performance of the MIL-100(Fe) coating OT capillary column.
Because of the size selectivity of lattice aperture and hydrophobicity of the organic ligands, three types of analytes were well
separated with this novel MIL-100(Fe) coating OT capillary column. For three consecutive runs, the intraday relative standard
deviations (RSDs) of migration time and peak areas were 0.4?4.6% and 1.2?6.6%, respectively. The interday RSDs of migration
time and peak areas were 0.6?8.0% and 2.2?9.5%, respectively. The column-to-column reproducibility of retention time was in
range of 0.6?9.2%. Additionally, the 10 cycles OT capillary column (10-LC) could be used for more than 150 runs with no
observable changes on the separation efficiency.