重组人CD73蛋白|CD73蛋白

产品编号: KMP2245

产品名称: Human CD73 Protein, Fc Tag

中文名称: 重组人CD73蛋白

中文全称: 重组人CD73蛋白

关键词: 5'-Nucleotidase, 5'-NT, Ecto-5'-Nucleotidase, CD73, NT5E, NT5, NTE, Human CD73 Protein, Fc Tag, Recombinant Protein, KMP2245

物种: Human

宿主: HEK293 Cells

规格: 50ug, 100ug, 200ug

纯化方式: Affinity purification

纯度: >95% as determined by SDS-PAGE

内毒素水平: <1.0 EU/ug determined by the LAL method

缓冲体系: 20mM TrisHCl, 120mM NaCl, 4mM CaCl2, 20% Glycerol, pH7.5

Uniprot: P21589

SDS-PAGE: KMP2245 SDS-PAGE image1.png

功能: Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate (PubMed:21933152, PubMed:34403084, PubMed:24887587, PubMed:22997138, PubMed:23142347).

Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (PubMed:34403084).

Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (PubMed:21933152, PubMed:34403084, PubMed:24887587, PubMed:22997138, PubMed:23142347).

产品背景: CD73 is a glycosyl phosphatidylinositol(GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity. Defects in NT5E are the cause of calcification of joints and arteries(CAJA). The recombinant CD73 lacking GPI anchor is secreted as a monomer.

保存条件: Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.

运输条件: In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.

说明: This product is for research use only.

官网链接: https://www.kmdbioscience.cn/

参考文献: 1.Biochem. J. 441:131-141 (2012)

2.ChemBioChem 13:2384-2391 (2012)

3.Structure 20:2161-2173 (2012)

4.PLoS ONE 9:E98568-E98568 (2014)

5.Purinergic Signal. 17:693-704 (2021)