This application note demonstrates that stopped-flow CD measurements can be used to measure the protein unfolding processes, analyze the secondary structure changes, and obtain reaction rate constants

这个应用文章主要将演示缓解和一滴血微量试样光盘通过测量的效率四个蛋白的CD光谱。

This application note monitors the existence of an intermediate state by probing the refolding of denatured cytochrome c using a JASCO CD spectrometer and the SFS-492 Stopped-Flow system.

Circular dichroism spectroscopy (CD) is very sensitive to the secondary structure of polypeptides and proteins. Circular dichroism is a form of light absorption spectroscopy that measures the difference in absorbance of rightand left-circularly polarized light (rather than the commonly used absorbance of isotropic light) by a substance. It has been shown that CD spectra between 260 and approximately 180 nm can be analyzed for the different secondary structure types: alpha helix, beta sheet, beta turn, random coils, etc. Secondary structure determination by CD is reported to achieve accuracies of 0.97 for helices, 0.75 for beta sheet, 0.50 for turns, and 0.89 for other structure types. In this application CD will be used to follow the denaturation of myoglobin during an automated pH titration with acid.

The functionalities of chiral compounds have been studied for the development of advanced molecules for many applications. The study of chiral compounds has spread to several fields such as natural products, pharmaceuticals and other functional molecules, and it can be pointed out that among those studies, the structural analysis of chiral compounds is a very important topic. X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR) and Electronic Circular Dichroism (ECD) using UV/Vis light are employed as primary methods for the structural analysis of chiral compounds. In this paper, the measurement of chiral compounds by Vibrational Circular Dichroism (VCD) using infrared light will be outlined.

This application note illustrates temperature-dependent CD and fluorescence measurements of lysozyme obtained simultaneously using the J-1500 CD spectrometer, FMO-522 Emission Monochromator accessory, and Temperature/Wavelength Scan Measurement program.

绿色荧光蛋白(GFP)是一种在紫外光照射下发出绿色荧光的蛋白质。此应用说明 用CPL-300和J-1500分别获得了gfp的cpl和cd光谱。

由于自动测量系统具有自动分析多个样品的能力，节省了时间和人力，越来越受到制药企业的重视。本文介绍了一种使用高通量圆二色光谱仪自动测量多个蛋白质样品的方法。

随着核磁共振和X射线结晶学的发展，圆二色谱是蛋白质结构分析中不可缺少的一种测量方法.对于CD样品的测量，小体积或低浓度是典型的要求，JASCO开发了一种新的采样技术来满足这些样品的要求。 此应用通过测量四种蛋白质的圆二色光谱来说明一滴微取样盘的简易性和有效性。