关注
已关注
已认证
粉丝量 0
400-827-8006
仪器信息网认证电话，请放心拨打
自动pH滴定期间肌红蛋白结构的CD检测
2019/12/22 15:00
阅读：224
分享：
方案摘要：
产品配置单：
JASCO日本分光 圆二色光谱仪/圆二色谱 J-1500
型号： J-1500
产地： 日本
品牌： 日本分光JASCO
¥130万
参考报价
联系电话
方案详情：
Circular dichroism spectroscopy (CD) is very sensitive to the secondary structure of polypeptides and proteins. Circular dichroism is a form of light absorption spectroscopy that measures the difference in absorbance of rightand left-circularly polarized light (rather than the commonly used absorbance of isotropic light) by a substance. It has been shown that CD spectra between 260 and approximately 180 nm can be analyzed for the different secondary structure types: alpha helix, beta sheet, beta turn, random coils, etc. Secondary structure determination by CD is reported to achieve accuracies of 0.97 for helices, 0.75 for beta sheet, 0.50 for turns, and 0.89 for other structure types. In this application CD will be used to follow the denaturation of myoglobin during an automated pH titration with acid.
下载本篇解决方案：
更多
使用CD分析安非他命及其对映异构体
鉴定合成副产物和确定活性物质的对映体组成以及制剂中存在的杂质，是法医和药理学分析的重要目的。 这对于比较和评价生产方法也是有用的。 在本应用中，我们将展示CD在对映体组成测定中的适用性。
制药/生物制药
2021/01/21
使用圆二色自动滴定装置分析人血清白蛋白与水杨酸相互作用
This application notes demonstrates the use of the J-1500 CD spectrometer and ATS-530 automatic titrator to monitor the interaction of HSA titrated with 3,5-diiodosalicylic acid. While 3,5-diiodosalicylic acid is achiral, its interaction with chiral HSA induces circular dichroism.
医疗/卫生
2020/09/09
使用停留圆二色光谱研究三氟乙醇对刀豆蛋白A的展开
This application note demonstrates that stopped-flow CD measurements can be used to measure the protein unfolding processes, analyze the secondary structure changes, and obtain reaction rate constants
生物产业
2020/04/08
