JASCO日本分光 圆二色光谱仪/圆二色谱 J-1500

鉴定合成副产物和确定活性物质的对映体组成以及制剂中存在的杂质，是法医和药理学分析的重要目的。 这对于比较和评价生产方法也是有用的。 在本应用中，我们将展示CD在对映体组成测定中的适用性。

The function of a protein or peptide is closely related to its structure. Since CD measurements probe amino acid structure changes, CD is widely used for the structural analysis of protein and peptide molecules formulated for pharmaceutical use. CD spectral shapes reflect the abundance ratio of secondary structure motifs in proteins and poly-peptides and secondary structure analysis of CD data provides a fast and accurate indication of change in these structures.

本应用介绍了如何使用圆二色光谱仪（CD）的多元SSE程序分析八种蛋白质的二级结构，并与x射线晶体学得到的二次结构结果吻合较好。

Human serum albumin (HSA) is the most abundant protein in blood plasma. HSA binds with pharmaceutical compounds and other in-vivo substances and plays an important role in the transport of these substances to target organs. Several studies have reported on the binding affinity of HSA and its interactions with a variety of compounds.

This application note demonstrates that stopped-flow CD measurements can be used to measure the protein unfolding processes, analyze the secondary structure changes, and obtain reaction rate constants

这个应用文章主要将演示缓解和一滴血微量试样光盘通过测量的效率四个蛋白的CD光谱。

This application note monitors the existence of an intermediate state by probing the refolding of denatured cytochrome c using a JASCO CD spectrometer and the SFS-492 Stopped-Flow system.

Circular dichroism spectroscopy (CD) is very sensitive to the secondary structure of polypeptides and proteins. Circular dichroism is a form of light absorption spectroscopy that measures the difference in absorbance of rightand left-circularly polarized light (rather than the commonly used absorbance of isotropic light) by a substance. It has been shown that CD spectra between 260 and approximately 180 nm can be analyzed for the different secondary structure types: alpha helix, beta sheet, beta turn, random coils, etc. Secondary structure determination by CD is reported to achieve accuracies of 0.97 for helices, 0.75 for beta sheet, 0.50 for turns, and 0.89 for other structure types. In this application CD will be used to follow the denaturation of myoglobin during an automated pH titration with acid.

This application note illustrates temperature-dependent CD and fluorescence measurements of lysozyme obtained simultaneously using the J-1500 CD spectrometer, FMO-522 Emission Monochromator accessory, and Temperature/Wavelength Scan Measurement program.

由于自动测量系统具有自动分析多个样品的能力，节省了时间和人力，越来越受到制药企业的重视。本文介绍了一种使用高通量圆二色光谱仪自动测量多个蛋白质样品的方法。

This application notes demonstrates the use of the J-1500 CD spectrometer and ATS-530 automatic titrator to monitor the interaction of HSA titrated with 3,5-diiodosalicylic acid. While 3,5-diiodosalicylic acid is achiral, its interaction with chiral HSA induces circular dichroism.

本应用说明了如何使用圆二色的光谱质控软件监测HSA的生物分子稳定性，并突出了光谱质控测试程序的特点。