This application note demonstrates how to determine a sample’s excitation and emission maxima for obtaining more indepth fluorescence measurements using the 3-D Fluorescence Measurement Program.

This application note demonstrates the measurement and analysis of camphorquinone using two complimentary techniques: circular dichroism and circularly polarized luminescence.

Atmospheric water vapor and carbon dioxide (CO2) strongly absorb in the infrared region and can interfere with the analysis and detection of weak sample peaks due to the additional peaks in the spectrum. In order to reduce the effect of the background peaks associated with H20 and CO2 it is important to maintain the concentration of water vapor and CO2 at the same level when measuring both the background and sample. In this application note, we would like to introduce a couple measurement options to minimize the effects of CO2 and water vapor, including “Shuttle Measurement” and “Purge and Vacuum”.

This application note demonstrates the high resolution CPL spectrum measurements of europium tris[3-(trifluorometh ylhydroxymethylene)-(+)-camphorate] (Eu(facam)3) using a CPL-300. Eu(facam)3 is a common NMR shift reagent and has been used as a standard for CPL measurements.2