抑癌基因抗体

抑癌基因抗体 

『别名』mammary serine proteinase inhibitor; Maspin; Ovalbumin; PI-5; PI5; Protease inhibitor 5; Serine (or cysteine) peptidase inhibitor, clade B, member 5; Serpin B5; serpin peptidase inhibitor; Serpin peptidase inhibitor, clade B (ovalbumin), member 5; SERPINB5; SPB5_HUMAN; Spi5; Spi7.  

『浓度』抑癌基因抗体1mg/1ml 

『规格』0.1ml/100μg  0.2ml/200μg     

『抗体来源』Rabbit  

抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展，抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务，并将成为您在科研及生产中的得力助手。

我公司免费提供抗血清亲合层析纯化服务，得到目的蛋白的抗体IgG。

我公司还免费提供真空冷冻抽干服务，得到冻干粉状的抗体IgG产品，以便产品的使用及长期保存。

抑癌基因抗体洗脱问题

抗体组目前采用的是低pH洗脱方法，按道理说，这个方法应该可以洗脱掉纯大部分抗体，但是有时候出现了一些顽固抗体很难洗脱，大家可以考虑其它的洗脱方法，beads说明书上提到了五大类办法，我觉得比较可靠的方法只有两类，一是改变pH，二是改变离子浓度，另外几种办法要么会带入有机试剂，要么会带入氨基，所以不太适用。改变pH的办法除了用低pH，还可以考虑用高pH来洗脱，好像没多少人尝试过这种办法，在确实没有办法的时候推荐尝试一下。 

抑癌基因抗体其它问题

1、看了很多抗体纯化的资料，不管是从血清中纯化多抗还是从腹水中纯化单抗，都推荐大家在上柱子之前把血清或腹水稀释2-5倍，另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了，但是稀释这一步推荐大家试一试，有文献说稀释可以提高回收率

2、封闭这一步做完后，是不是考虑可以用HCl洗一次？连在beads上的蛋白有的是蛋白间非特异性结合上去的，洗脱之前的任何步骤也许都无法洗干净它们，所以这里把它们都洗掉，以免最后都洗到抗体里去了。

3、CNBr活化的琼脂糖或葡聚糖本身的缺陷：这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白，其中包括我们要的抗体，它们都带有一定的负电，所以都会和这个部位进行非特异性结合，在洗脱前，有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的，但是它也会洗掉直接与beads非特异结合的蛋白，也包括我们要的抗体，至于这一步会丢掉多少抗体，好像没有人测过。

PAB947Hu01 顶体蛋白(ACR)多克隆抗体 Polyclonal Antibody to Acrosin (ACR)

PAB947Ra01 顶体蛋白(ACR)多克隆抗体 Polyclonal Antibody to Acrosin (ACR)

PAJ683Hu01 顺乌头酸酶1(ACO1)多克隆抗体 Polyclonal Antibody to Aconitase 1 (ACO1)

PAJ683Mu01 顺乌头酸酶1(ACO1)多克隆抗体 Polyclonal Antibody to Aconitase 1 (ACO1)

PAJ683Hu03 顺乌头酸酶1(ACO1)多克隆抗体 Polyclonal Antibody to Aconitase 1 (ACO1)

PAJ683Hu04 顺乌头酸酶1(ACO1)多克隆抗体 Polyclonal Antibody to Aconitase 1 (ACO1)

PAJ683Hu02 顺乌头酸酶1(ACO1)多克隆抗体 Polyclonal Antibody to Aconitase 1 (ACO1)

PAJ683Mu02 顺乌头酸酶1(ACO1)多克隆抗体 Polyclonal Antibody to Aconitase 1 (ACO1)

PAJ683Ra01 顺乌头酸酶1(ACO1)多克隆抗体 Polyclonal Antibody to Aconitase 1 (ACO1)

PAD812Hu01 酸性唾液脯氨酸丰富磷蛋白2(PRH2)多克隆抗体 Polyclonal Antibody to Acidic Salivary Proline Rich Phosphoprotein 2 (PRH2)

PAJ636Hu01 酸性核磷蛋白32A(ANP32A)多克隆抗体 Polyclonal Antibody to Acidic Nuclear Phosphoprotein 32 Family, Member A (ANP32A)

PAJ636Mu01 酸性核磷蛋白32A(ANP32A)多克隆抗体 Polyclonal Antibody to Acidic Nuclear Phosphoprotein 32 Family, Member A (ANP32A)

PAB360Hu01 酸性神经鞘磷脂酶(ASM)多克隆抗体 Polyclonal Antibody to Acid Sphingomyelinase (ASM)

PAA902Hu01 耐酒石酸酸性磷酸酶(ACP5)多克隆抗体 Polyclonal Antibody to Acid Phosphatase 5, Tartrate Resistant (ACP5)

PAA902Mu01 耐酒石酸酸性磷酸酶(ACP5)多克隆抗体 Polyclonal Antibody to Acid Phosphatase 5, Tartrate Resistant (ACP5)

PAA902Ra01 耐酒石酸酸性磷酸酶(ACP5)多克隆抗体 Polyclonal Antibody to Acid Phosphatase 5, Tartrate Resistant (ACP5)

PAA902Rb51 耐酒石酸酸性磷酸酶(ACP5)多克隆抗体 Polyclonal Antibody to Acid Phosphatase 5, Tartrate Resistant (ACP5)

PAA902Mu02 耐酒石酸酸性磷酸酶(ACP5)多克隆抗体 Polyclonal Antibody to Acid Phosphatase 5, Tartrate Resistant (ACP5)

PAA168Hu01 前列腺酸性磷酸酶(ACP3)多克隆抗体 Polyclonal Antibody to Acid Phosphatase 3, Prostatic (ACP3)

PAA168Mu01 前列腺酸性磷酸酶(ACP3)多克隆抗体 Polyclonal Antibody to Acid Phosphatase 3, Prostatic (ACP3)

PAA168Hu02 前列腺酸性磷酸酶(ACP3)多克隆抗体 Polyclonal Antibody to Acid Phosphatase 3, Prostatic (ACP3)

PAD089Hu01 溶酶体酸性磷酸酶2(ACP2)多克隆抗体 Polyclonal Antibody to Acid Phosphatase 2, Lysosomal (ACP2)

PAD089Mu01 溶酶体酸性磷酸酶2(ACP2)多克隆抗体 Polyclonal Antibody to Acid Phosphatase 2, Lysosomal (ACP2)

PAD089Mu02 溶酶体酸性磷酸酶2(ACP2)多克隆抗体 Polyclonal Antibody to Acid Phosphatase 2, Lysosomal (ACP2)

PAA994Hu01 酸性磷酸酶1(ACP1)多克隆抗体 Polyclonal Antibody to Acid Phosphatase 1 (ACP1)

PAA994Ra01 酸性磷酸酶1(ACP1)多克隆抗体 Polyclonal Antibody to Acid Phosphatase 1 (ACP1)

PAA994Hu02 酸性磷酸酶1(ACP1)多克隆抗体 Polyclonal Antibody to Acid Phosphatase 1 (ACP1)

PAJ867Hu01 无刚毛鳞甲复合体样蛋白1(ASCL1)多克隆抗体 Polyclonal Antibody to Achaete Scute Complex Like Protein 1 (ASCL1)

PAD496Hu01 乙酰复合胺-O-甲基转移酶(ASMT)多克隆抗体 Polyclonal Antibody to Acetylserotonin-O-Methyltransferase (ASMT)

PAJ684Hu01 乙酰胆碱酯酶关联蛋白(ACHAP)多克隆抗体 Polyclonal Antibody to Acetylcholinesterase Associated Protein (ACHAP)

PAB447Ra01 乙酰胆碱酯酶(AchE)多克隆抗体 Polyclonal Antibody to Acetylcholinesterase (AchE)

PAB447Mu01 乙酰胆碱酯酶(AchE)多克隆抗体 Polyclonal Antibody to Acetylcholinesterase (AchE)

PAB447Mu02 乙酰胆碱酯酶(AchE)多克隆抗体 Polyclonal Antibody to Acetylcholinesterase (AchE)

PAB447Hu01 乙酰胆碱酯酶(AchE)多克隆抗体 Polyclonal Antibody to Acetylcholinesterase (AchE)

PAB447Ra02 乙酰胆碱酯酶(AchE)多克隆抗体 Polyclonal Antibody to Acetylcholinesterase (AchE)

PAA912Ge01 乙酰胆碱(ACH)多克隆抗体 Polyclonal Antibody to Acetylcholine (ACH)

PAB284Mu01 乙酰辅酶A羧化酶α(ACACα)多克隆抗体 Polyclonal Antibody to Acetyl Coenzyme A Carboxylase Alpha (ACACa)

PAB284Hu01 乙酰辅酶A羧化酶α(ACACα)多克隆抗体 Polyclonal Antibody to Acetyl Coenzyme A Carboxylase Alpha (ACACa)

PAB284Mu02 乙酰辅酶A羧化酶α(ACACα)多克隆抗体 Polyclonal Antibody to Acetyl Coenzyme A Carboxylase Alpha (ACACa)

PAB284Ra01 乙酰辅酶A羧化酶α(ACACα)多克隆抗体 Polyclonal Antibody to Acetyl Coenzyme A Carboxylase Alpha (ACACa)

PAD665Hu01 乙酰辅酶A乙酰转移酶2(ACAT2)多克隆抗体 Polyclonal Antibody to Acetyl Coenzyme A Acetyltransferase 2 (ACAT2)

PAD665Mu01 乙酰辅酶A乙酰转移酶2(ACAT2)多克隆抗体 Polyclonal Antibody to Acetyl Coenzyme A Acetyltransferase 2 (ACAT2)

PAD665Mu02 乙酰辅酶A乙酰转移酶2(ACAT2)多克隆抗体 Polyclonal Antibody to Acetyl Coenzyme A Acetyltransferase 2 (ACAT2)

PAD664Mu01 乙酰辅酶A乙酰转移酶1(ACAT1)多克隆抗体 Polyclonal Antibody to Acetyl Coenzyme A Acetyltransferase 1 (ACAT1)

PAD664Mu02 乙酰辅酶A乙酰转移酶1(ACAT1)多克隆抗体 Polyclonal Antibody to Acetyl Coenzyme A Acetyltransferase 1 (ACAT1)

PAG302Hu01 黑素瘤缺乏因子2(AIM2)多克隆抗体 Polyclonal Antibody to Absent In Melanoma 2 (AIM2)

PAG301Hu01 黑素瘤缺乏因子1(AIM1)多克隆抗体 Polyclonal Antibody to Absent In Melanoma 1 (AIM1)

PAB218Ge01 激素脱落酸(ABA)多克隆抗体 Polyclonal Antibody to Abscisic Acid (ABA)

PAJ696Hu01 Abl交互子1(ABI1)多克隆抗体 Polyclonal Antibody to Abl Interactor 1 (ABI1)

PAE892Hu01 激酶锚定蛋白12(AKAP12)多克隆抗体 Polyclonal Antibody to A Kinase Anchor Protein 12 (AKAP12)

PAC197Hu01 激酶锚定蛋白11(AKAP11)多克隆抗体 Polyclonal Antibody to A Kinase Anchor Protein 11 (AKAP11)

PAK209Hu01 血小板反应蛋白解整合素金属肽酶9(ADAMTS9)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 9 (ADAMTS9)

PAK208Hu01 血小板反应蛋白解整合素金属肽酶8(ADAMTS8)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 8 (ADAMTS8)

PAB974Hu01 血小板反应蛋白解整合素金属肽酶7(ADAMTS7)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7)

PAB974Ra01 血小板反应蛋白解整合素金属肽酶7(ADAMTS7)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7)

PAB974Mu01 抑癌基因抗体血小板反应蛋白解整合素金属肽酶7(ADAMTS7)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7)

PAK205Hu01 血小板反应蛋白解整合素金属肽酶5(ADAMTS5)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5)

PAK205Ra01 血小板反应蛋白解整合素金属肽酶5(ADAMTS5)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5)

PAK204Hu01 血小板反应蛋白解整合素金属肽酶4(ADAMTS4)多克隆抗体 Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4)