?自噬微管相关蛋白轻链3抗体
『别名』MAP1LC3A; MAP1a + MAP1b; microtubule-associated protein 1 light chain 3; Autophagy LC3; Microtubule-associated proteins 1A/1B light chain 3A; MAPLC3; MAP-LC3; Microtubule-associated protein 1 light chain 3 alpha; MAP1A/1B light chain 3 A; MAP1A/MAP1B LC3 A; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1.MAP1LC3A
『浓度』自噬微管相关蛋白轻链3抗体1mg/1ml
『规格』0.1ml/100μg 0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
自噬微管相关蛋白轻链3抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
自噬微管相关蛋白轻链3抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
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PAB557Ra01 失调蛋白2结合蛋白1(A2BP1)多克隆抗体 Polyclonal Antibody to Ataxin 2 Binding Protein 1 (A2BP1)
PAD248Mu01 失调蛋白10(ATXN10)多克隆抗体 Polyclonal Antibody to Ataxin 10 (ATXN10)
PAD248Mu02 失调蛋白10(ATXN10)多克隆抗体 Polyclonal Antibody to Ataxin 10 (ATXN10)
PAD248Hu01 失调蛋白10(ATXN10)多克隆抗体 Polyclonal Antibody to Ataxin 10 (ATXN10)
PAA514Hu01 失调蛋白1(ATXN1)多克隆抗体 Polyclonal Antibody to Ataxin 1 (ATXN1)
PAA514Mu01 失调蛋白1(ATXN1)多克隆抗体 Polyclonal Antibody to Ataxin 1 (ATXN1)
PAB361Hu01 毛细血管扩张性共济失调突变因子(ATM)多克隆抗体 Polyclonal Antibody to Ataxia Telangiectasia Mutated (ATM)
PAB361Mu01 毛细血管扩张性共济失调突变因子(ATM)多克隆抗体 Polyclonal Antibody to Ataxia Telangiectasia Mutated (ATM)
PAB361Mu02 毛细血管扩张性共济失调突变因子(ATM)多克隆抗体 Polyclonal Antibody to Ataxia Telangiectasia Mutated (ATM)
PAK284Hu01 共济失调毛细血管扩张Rad3相关蛋白(ATR)多克隆抗体 Polyclonal Antibody to Ataxia Telangiectasia And Rad3 Related Protein (ATR)
PAB301Ge01 不对称二甲基精氨酸(ADMA)多克隆抗体 Polyclonal Antibody to Asymmetrical Dimethylarginine (ADMA)
PAJ593Hu01 龙虾肽酶样金属内肽酶(ASTL)多克隆抗体 Polyclonal Antibody to Astacin Like Metallo Endopeptidase (ASTL)
PAC321Hu01 无孢蛋白(ASPN)多克隆抗体 Polyclonal Antibody to Asporin (ASPN)
PAC321Mu01 无孢蛋白(ASPN)多克隆抗体 Polyclonal Antibody to Asporin (ASPN)
PAC321Mu02 无孢蛋白(ASPN)多克隆抗体 Polyclonal Antibody to Asporin (ASPN)
PAG151Hu01 天冬氨酸β羟化酶(ASPH)多克隆抗体 Polyclonal Antibody to Aspartate Beta Hydroxylase (ASPH)
PAD853Hu01 天冬氨酸转氨酶2(AST2)多克隆抗体 Polyclonal Antibody to Aspartate Aminotransferase 2 (AST2)
PAD853Mu01 天冬氨酸转氨酶2(AST2)多克隆抗体 Polyclonal Antibody to Aspartate Aminotransferase 2 (AST2)
PAD853Mu02 天冬氨酸转氨酶2(AST2)多克隆抗体 Polyclonal Antibody to Aspartate Aminotransferase 2 (AST2)
PAB214Hu01 天冬氨酸转氨酶(AST)多克隆抗体 Polyclonal Antibody to Aspartate Aminotransferase (AST)
PAB214Mu01 天冬氨酸转氨酶(AST)多克隆抗体 Polyclonal Antibody to Aspartate Aminotransferase (AST)
PAB214Ra01 天冬氨酸转氨酶(AST)多克隆抗体 Polyclonal Antibody to Aspartate Aminotransferase (AST)
PAB214Bo01 天冬氨酸转氨酶(AST)多克隆抗体 Polyclonal Antibody to Aspartate Aminotransferase (AST)
PAB214Mu02 天冬氨酸转氨酶(AST)多克隆抗体 Polyclonal Antibody to Aspartate Aminotransferase (AST)
PAB214Hu02 天冬氨酸转氨酶(AST)多克隆抗体 Polyclonal Antibody to Aspartate Aminotransferase (AST)
PAB214Hu51 天冬氨酸转氨酶(AST)多克隆抗体 Polyclonal Antibody to Aspartate Aminotransferase (AST)
PAD760Hu01 天冬酰胺合成酶(ASNS)多克隆抗体 Polyclonal Antibody to Asparagine Synthetase (ASNS)
PAD760Mu01 天冬酰胺合成酶(ASNS)多克隆抗体 Polyclonal Antibody to Asparagine Synthetase (ASNS)
PAD760Mu02 天冬酰胺合成酶(ASNS)多克隆抗体 Polyclonal Antibody to Asparagine Synthetase (ASNS)
PAC067Hu01 去唾液酸糖蛋白受体2(ASGR2)多克隆抗体 Polyclonal Antibody to Asialoglycoprotein Receptor 2 (ASGR2)
PAB189Hu01 去唾液酸糖蛋白受体1(ASGPR1)多克隆抗体 Polyclonal Antibody to Asialoglycoprotein Receptor 1 (ASGR1)
PAB189Mu01 去唾液酸糖蛋白受体1(ASGPR1)多克隆抗体 Polyclonal Antibody to Asialoglycoprotein Receptor 1 (ASGR1)
PAE405Hu01 Asc型氨基酸转运蛋白1(ASC1)多克隆抗体 Polyclonal Antibody to Asc Type Amino Acid Transporter 1 (ASC1)
PAG613Hu01 芳基硫酸酯酶F(ARSF)多克隆抗体 Polyclonal Antibody to Arylsulfatase F (ARSF)
PAG610Hu01 芳基硫酸酯酶B(ARSB)多克隆抗体 Polyclonal Antibody to Arylsulfatase B (ARSB)
PAG619Hu01 芳基硫酸酯酶A(ARSA)多克隆抗体 Polyclonal Antibody to Arylsulfatase A (ARSA)
PAJ665Hu01 芳犬尿氨酸甲酰胺酶(AFMID)多克隆抗体 Polyclonal Antibody to Arylformamidase (AFMID)
PAB937Hu01 芳香胺-N-乙酰基转移酶(AANAT)多克隆抗体 Polyclonal Antibody to Arylalkylamine-N-Acetyltransferase (AANAT)
PAJ660Hu01 芳烃受体抑制因子(AHRR)多克隆抗体 Polyclonal Antibody to Aryl Hydrocarbon Receptor Repressor (AHRR)
PAD468Ra01 芳香烃受体核转位因子样蛋白(ARNTL)多克隆抗体 Polyclonal Antibody to Aryl Hydrocarbon Receptor Nuclear Translocator Like Protein (ARNTL)
PAD470Mu01 芳香烃受体核转位因子(ARNT)多克隆抗体 Polyclonal Antibody to Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT)
PAD470Hu01 芳香烃受体核转位因子(ARNT)多克隆抗体 Polyclonal Antibody to Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT)
PAD470Mu02 芳香烃受体核转位因子(ARNT)多克隆抗体 Polyclonal Antibody to Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT)
PAB354Hu01 芳香烃受体(AhR)多克隆抗体 Polyclonal Antibody to Aryl Hydrocarbon Receptor (AhR)
PAB354Mu01 芳香烃受体(AhR)多克隆抗体 Polyclonal Antibody to Aryl Hydrocarbon Receptor (AhR)
PAB354Ra01 芳香烃受体(AhR)多克隆抗体 Polyclonal Antibody to Aryl Hydrocarbon Receptor (AhR)
PAJ596Hu01 神经鞘胚素(ARTN)多克隆抗体 Polyclonal Antibody to Artemin (ARTN)
PAJ596Mu01 神经鞘胚素(ARTN)多克隆抗体 Polyclonal Antibody to Artemin (ARTN)
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PAB993Mu01 抑制蛋白β1(ARRβ1)多克隆抗体 Polyclonal Antibody to Arrestin Beta 1 (ARRb1)
PAB993Mu02 抑制蛋白β1(ARRβ1)多克隆抗体 Polyclonal Antibody to Arrestin Beta 1 (ARRb1)
PAB993Hu01 抑制蛋白β1自噬微管相关蛋白轻链3抗体(ARRβ1)多克隆抗体 Polyclonal Antibody to Arrestin Beta 1 (ARRb1)
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