肌球蛋白结合蛋白C抗体

?肌球蛋白结合蛋白C抗体 

『别名』skeletal muscle slow isoform; slow-type; C protein, skeletal muscle slow isoform; C-protein; MYBPC1; MYBPCC; MYBPCS; Myosin binding protein C, slow type; Myosin-binding protein C; MYPC1_HUMAN; skeletal muscle C protein; Slow MyBP C; Slow MyBP-C. 

『浓度』肌球蛋白结合蛋白C抗体1mg/1ml 

『规格』0.2ml/200μg     

『抗体来源』Rabbit  

抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展，抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务，并将成为您在科研及生产中的得力助手。

我公司免费提供抗血清亲合层析纯化服务，得到目的蛋白的抗体IgG。

我公司还免费提供真空冷冻抽干服务，得到冻干粉状的抗体IgG产品，以便产品的使用及长期保存。

肌球蛋白结合蛋白C抗体洗脱问题

抗体组目前采用的是低pH洗脱方法，按道理说，这个方法应该可以洗脱掉纯大部分抗体，但是有时候出现了一些顽固抗体很难洗脱，大家可以考虑其它的洗脱方法，beads说明书上提到了五大类办法，我觉得比较可靠的方法只有两类，一是改变pH，二是改变离子浓度，另外几种办法要么会带入有机试剂，要么会带入氨基，所以不太适用。改变pH的办法除了用低pH，还可以考虑用高pH来洗脱，好像没多少人尝试过这种办法，在确实没有办法的时候推荐尝试一下。 

肌球蛋白结合蛋白C抗体其它问题

1、看了很多抗体纯化的资料，不管是从血清中纯化多抗还是从腹水中纯化单抗，都推荐大家在上柱子之前把血清或腹水稀释2-5倍，另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了，但是稀释这一步推荐大家试一试，有文献说稀释可以提高回收率

2、封闭这一步做完后，是不是考虑可以用HCl洗一次？连在beads上的蛋白有的是蛋白间非特异性结合上去的，洗脱之前的任何步骤也许都无法洗干净它们，所以这里把它们都洗掉，以免最后都洗到抗体里去了。

3、CNBr活化的琼脂糖或葡聚糖本身的缺陷：这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白，其中包括我们要的抗体，它们都带有一定的负电，所以都会和这个部位进行非特异性结合，在洗脱前，有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的，但是它也会洗掉直接与beads非特异结合的蛋白，也包括我们要的抗体，至于这一步会丢掉多少抗体，好像没有人测过。

PAB212Hu01 嗜铬蛋白A(CHGA)多克隆抗体 Polyclonal Antibody to Chromogranin A (CHGA)

PAB212Ra01 嗜铬蛋白A(CHGA)多克隆抗体 Polyclonal Antibody to Chromogranin A (CHGA)

PAB212Mu01 嗜铬蛋白A(CHGA)多克隆抗体 Polyclonal Antibody to Chromogranin A (CHGA)

PAB212Po01 嗜铬蛋白A(CHGA)多克隆抗体 Polyclonal Antibody to Chromogranin A (CHGA)

PAC211Hu01 染色质域解旋酶DNA结合蛋白5(CHD5)多克隆抗体 Polyclonal Antibody to Chromodomain Helicase DNA Binding Protein 5 (CHD5)

PAD202Mu01 染色质域解旋酶DNA结合蛋白4(CHD4)多克隆抗体 Polyclonal Antibody to Chromodomain Helicase DNA Binding Protein 4 (CHD4)

PAD202Mu02 染色质域解旋酶DNA结合蛋白4(CHD4)多克隆抗体 Polyclonal Antibody to Chromodomain Helicase DNA Binding Protein 4 (CHD4)

PAA317Hu01 染色质域解旋酶DNA结合蛋白3(CHD3)多克隆抗体 Polyclonal Antibody to Chromodomain Helicase DNA Binding Protein 3 (CHD3)

PAA317Mu01 染色质域解旋酶DNA结合蛋白3(CHD3)多克隆抗体 Polyclonal Antibody to Chromodomain Helicase DNA Binding Protein 3 (CHD3)

PAC363Hu01 染色框同源物3(CBX3)多克隆抗体 Polyclonal Antibody to Chromobox Homolog 3 (CBX3)

PAC363Mu01 染色框同源物3(CBX3)多克隆抗体 Polyclonal Antibody to Chromobox Homolog 3 (CBX3)

PAJ447Mu01 染色质装配因子1亚基B(CHAF1B)多克隆抗体 Polyclonal Antibody to Chromatin Assembly Factor 1, Subunit B (CHAF1B)

PAJ447Mu02 染色质装配因子1亚基B(CHAF1B)多克隆抗体 Polyclonal Antibody to Chromatin Assembly Factor 1, Subunit B (CHAF1B)

PAD215Hu01 绒毛膜促性腺激素β肽(CGβ)多克隆抗体 Polyclonal Antibody to Chorionic Gonadotropin Beta Polypeptide (CGb)

PAB612Hu01 绒毛膜促性腺激素α肽(CGα)多克隆抗体 Polyclonal Antibody to Chorionic Gonadotropin Alpha Polypeptide (CGa)

PAB612Mu01 绒毛膜促性腺激素α肽(CGα)多克隆抗体 Polyclonal Antibody to Chorionic Gonadotropin Alpha Polypeptide (CGa)

PAD216Hu01 绒毛膜促性腺激素(CG)多克隆抗体 Polyclonal Antibody to Chorionic Gonadotropin (CG)

PAF626Mu01 腱蛋白样蛋白2(CHRDL2)多克隆抗体 Polyclonal Antibody to Chordin Like Protein 2 (CHRDL2)

PAF626Mu02 腱蛋白样蛋白2(CHRDL2)多克隆抗体 Polyclonal Antibody to Chordin Like Protein 2 (CHRDL2)

PAF625Hu01 腱蛋白样蛋白1(CHRDL1)多克隆抗体 Polyclonal Antibody to Chordin Like Protein 1 (CHRDL1)

PAF625Mu01 腱蛋白样蛋白1(CHRDL1)多克隆抗体 Polyclonal Antibody to Chordin Like Protein 1 (CHRDL1)

PAC126Ra01 腱蛋白(CHRD)多克隆抗体 Polyclonal Antibody to Chordin (CHRD)

PAC126Mu01 腱蛋白(CHRD)多克隆抗体 Polyclonal Antibody to Chordin (CHRD)

PAC126Hu01 腱蛋白(CHRD)多克隆抗体 Polyclonal Antibody to Chordin (CHRD)

PAB141Ra01 硫酸软骨素蛋白多糖5(CSPG5)多克隆抗体 Polyclonal Antibody to Chondroitin Sulfate Proteoglycan 5 (CSPG5)

PAA723Ge01 硫酸软骨素(CS)多克隆抗体 Polyclonal Antibody to Chondroitin Sulfate (CS)

PAD144Hu01 烟碱型胆碱受体β3(CHRNβ3)多克隆抗体 Polyclonal Antibody to Cholinergic Receptor, Nicotinic, Beta 3 (CHRNb3)

PAB513Hu01 烟碱型胆碱受体β2(CHRNβ2)多克隆抗体 Polyclonal Antibody to Cholinergic Receptor, Nicotinic, Beta 2 (CHRNb2)

PAB648Ra01 烟碱型胆碱受体β1(CHRNβ1)多克隆抗体 Polyclonal Antibody to Cholinergic Receptor, Nicotinic, Beta 1 (CHRNb1)

PAC305Hu01 烟碱型胆碱受体α7(CHRNα7)多克隆抗体 Polyclonal Antibody to Cholinergic Receptor, Nicotinic, Alpha 7 (CHRNa7)

PAD142Hu01 烟碱型胆碱受体α5(CHRNα5)多克隆抗体 Polyclonal Antibody to Cholinergic Receptor, Nicotinic, Alpha 5 (CHRNa5)

PAD143Hu01 烟碱型胆碱受体α3(CHRNα3)多克隆抗体 Polyclonal Antibody to Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3)

PAA981Ra01 烟碱型胆碱受体α1(CHRNα1)多克隆抗体 Polyclonal Antibody to Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1)

PAA981Hu01 烟碱型胆碱受体α1(CHRNα1)多克隆抗体 Polyclonal Antibody to Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1)

PAB838Mu01 毒蕈碱型胆碱受体M3(CHRM3)多克隆抗体 Polyclonal Antibody to Cholinergic Receptor, Muscarinic 3 (CHRM3)

PAB838Mu02 毒蕈碱型胆碱受体M3(CHRM3)多克隆抗体 Polyclonal Antibody to Cholinergic Receptor, Muscarinic 3 (CHRM3)

PAB838Hu01 毒蕈碱型胆碱受体M3(CHRM3)多克隆抗体 Polyclonal Antibody to Cholinergic Receptor, Muscarinic 3 (CHRM3)

PAB213Hu01 毒蕈碱型胆碱受体M2(CHRM2)多克隆抗体 Polyclonal Antibody to Cholinergic Receptor, Muscarinic 2 (CHRM2)

PAB213Mu01 毒蕈碱型胆碱受体M2(CHRM2)多克隆抗体 Polyclonal Antibody to Cholinergic Receptor, Muscarinic 2 (CHRM2)

PAB213Ra01 毒蕈碱型胆碱受体M2(CHRM2)多克隆抗体 Polyclonal Antibody to Cholinergic Receptor, Muscarinic 2 (CHRM2)

PAB213Mu02 毒蕈碱型胆碱受体M2(CHRM2)多克隆抗体 Polyclonal Antibody to Cholinergic Receptor, Muscarinic 2 (CHRM2)

PAA982Ra01 毒蕈碱型胆碱受体M1(CHRM1)多克隆抗体 Polyclonal Antibody to Cholinergic Receptor, Muscarinic 1 (CHRM1)

PAB929Mu01 胆碱乙酰转移酶(ChAT)多克隆抗体 Polyclonal Antibody to Choline Acetyltransferase (ChAT)

PAB929Ra01 胆碱乙酰转移酶(ChAT)多克隆抗体 Polyclonal Antibody to Choline Acetyltransferase (ChAT)

PAB929Hu01 胆碱乙酰转移酶(ChAT)多克隆抗体 Polyclonal Antibody to Choline Acetyltransferase (ChAT)

PAB929Mu02 胆碱乙酰转移酶(ChAT)多克隆抗体 Polyclonal Antibody to Choline Acetyltransferase (ChAT)

PAS088Ge01 胆酸(CA)多克隆抗体 Polyclonal Antibody to Cholic Acid (CA)

PAA814Hu01 脂质转运蛋白(CETP)多克隆抗体 Polyclonal Antibody to Cholesteryl Ester Transfer Protein (CETP)

PAA814Rb52 脂质转运蛋白(CETP)多克隆抗体 Polyclonal Antibody to Cholesteryl Ester Transfer Protein (CETP)

PAA814Hu02 脂质转运蛋白(CETP)多克隆抗体 Polyclonal Antibody to Cholesteryl Ester Transfer Protein (CETP)

PAA814Rb51 脂质转运蛋白肌球蛋白结合蛋白C抗体(CETP)多克隆抗体 Polyclonal Antibody to Cholesteryl Ester Transfer Protein (CETP)

PAA814Hu03 脂质转运蛋白(CETP)多克隆抗体 Polyclonal Antibody to Cholesteryl Ester Transfer Protein (CETP)

PAG357Hu01 胆固醇-25-羟化酶(CH25H)多克隆抗体 Polyclonal Antibody to Cholesterol-25-Hydroxylase (CH25H)

PAB701Ge01 胆固醇(CH)多克隆抗体 Polyclonal Antibody to Cholesterol (CH)