E3连接酶MMS21蛋白抗体
『别名』C8orf36; E3 SUMO-protein ligase NSE2; hMMS21; hNSE2; methyl methanesulfonate sensitivity gene 21; MMS21; MMS21 homolog; non-SMC element 2; Non-SMC element 2 homolog; Non-structural maintenance of chromosomes element 2 homolog; NSE2_HUMAN; NSMCE2.
『浓度』E3连接酶MMS21蛋白抗体1mg/1ml
『规格』0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
E3连接酶MMS21蛋白抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
E3连接酶MMS21蛋白抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
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PAA572Ga01 Ⅱ型胶原(COL2)多克隆抗体 Polyclonal Antibody to Collagen Type II (COL2)
PAA572Gu01 Ⅱ型胶原(COL2)多克隆抗体 Polyclonal Antibody to Collagen Type II (COL2)
PAA572Rb51 Ⅱ型胶原(COL2)多克隆抗体 Polyclonal Antibody to Collagen Type II (COL2)
PAA572Hu01 Ⅱ型胶原(COL2)多克隆抗体 Polyclonal Antibody to Collagen Type II (COL2)
PAA572Ra01 Ⅱ型胶原(COL2)多克隆抗体 Polyclonal Antibody to Collagen Type II (COL2)
PAA572Mu01 Ⅱ型胶原(COL2)多克隆抗体 Polyclonal Antibody to Collagen Type II (COL2)
PAA215Bo01 Ⅰ型胶原α2(COL1α2)多克隆抗体 Polyclonal Antibody to Collagen Type I Alpha 2 (COL1a2)
PAA215Ca01 Ⅰ型胶原α2(COL1α2)多克隆抗体 Polyclonal Antibody to Collagen Type I Alpha 2 (COL1a2)
PAA215Ga01 Ⅰ型胶原α2(COL1α2)多克隆抗体 Polyclonal Antibody to Collagen Type I Alpha 2 (COL1a2)
PAA215Hu01 Ⅰ型胶原α2(COL1α2)多克隆抗体 Polyclonal Antibody to Collagen Type I Alpha 2 (COL1a2)
PAA215Rb51 Ⅰ型胶原α2(COL1α2)多克隆抗体 Polyclonal Antibody to Collagen Type I Alpha 2 (COL1a2)
PAA215Mu01 Ⅰ型胶原α2(COL1α2)多克隆抗体 Polyclonal Antibody to Collagen Type I Alpha 2 (COL1a2)
PAA350Mu01 Ⅰ型胶原α1(COL1α1)多克隆抗体 Polyclonal Antibody to Collagen Type I Alpha 1 (COL1a1)
PAA350Ra01 Ⅰ型胶原α1(COL1α1)多克隆抗体 Polyclonal Antibody to Collagen Type I Alpha 1 (COL1a1)
PAA350Mu02 Ⅰ型胶原α1(COL1α1)多克隆抗体 Polyclonal Antibody to Collagen Type I Alpha 1 (COL1a1)
PAA350Hu02 Ⅰ型胶原α1(COL1α1)多克隆抗体 Polyclonal Antibody to Collagen Type I Alpha 1 (COL1a1)
PAA350Hu01 Ⅰ型胶原α1(COL1α1)多克隆抗体 Polyclonal Antibody to Collagen Type I Alpha 1 (COL1a1)
PAA571Hu01 Ⅰ型胶原(COL1)多克隆抗体 Polyclonal Antibody to Collagen Type I (COL1)
PAA571Ra01 Ⅰ型胶原(COL1)多克隆抗体 Polyclonal Antibody to Collagen Type I (COL1)
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PAE178Hu01 胰辅脂酶(CLPS)多克隆抗体 Polyclonal Antibody to Colipase, Pancreatic (CLPS)
PAE178Mu01 胰辅脂酶(CLPS)多克隆抗体 Polyclonal Antibody to Colipase, Pancreatic (CLPS)
PAE178Ra01 胰辅脂酶(CLPS)多克隆抗体 Polyclonal Antibody to Colipase, Pancreatic (CLPS)
PAE178Po01 胰辅脂酶(CLPS)多克隆抗体 Polyclonal Antibody to Colipase, Pancreatic (CLPS)
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PAJ901Ra01 含卷曲螺旋域蛋白80(CCDC80)多克隆抗体 Polyclonal Antibody to Coiled Coil Domain Containing Protein 80 (CCDC80)
PAJ901Hu02 含卷曲螺旋域蛋白80(CCDC80)多克隆抗体 Polyclonal Antibody to Coiled Coil Domain Containing Protein 80 (CCDC80)
PAU807Mu01 含卷曲螺旋域蛋白60(CCDC60)多克隆抗体 Polyclonal Antibody to Coiled Coil Domain Containing Protein 60 (CCDC60)
PAU807Ra01 含卷曲螺旋域蛋白60(CCDC60)多克隆抗体 Polyclonal Antibody to Coiled Coil Domain Containing Protein 60 (CCDC60)
PAU807Hu01 含卷曲螺旋域蛋白60(CCDC60)多克隆抗体 Polyclonal Antibody to Coiled Coil Domain Containing Protein 60 (CCDC60)
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PAB861Hu01 丝切蛋白2(CFL2)多克隆抗体 Polyclonal Antibody to Cofilin 2, Muscle (CFL2)
PAB559Hu01 丝切蛋白1(CFL1)多克隆抗体 Polyclonal Antibody to Cofilin 1, Non Muscle (CFL1)
PAB559Mu01 丝切蛋白1(CFL1)多克隆抗体 Polyclonal Antibody to Cofilin 1, Non Muscle (CFL1)
PAB559Mu02 丝切蛋白1(CFL1)多克隆抗体 Polyclonal Antibody to Cofilin 1, Non Muscle (CFL1)
PAB559Ra01 丝切蛋白1(CFL1)多克隆抗体 Polyclonal Antibody to Cofilin 1, Non Muscle (CFL1)
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PAB613Hu01 凝血因子ⅩⅢB肽(F13B)多克隆抗体 Polyclonal Antibody to Coagulation Factor XIII B Polypeptide (F13B)
PAB094Hu01 凝血因子ⅩⅢA1肽(F13A1)多克隆抗体 Polyclonal Antibody to Coagulation Factor XIII A1 Polypeptide (F13A1)
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