人抗类固醇生成细胞抗体ELISA试剂盒厂家
我们免费代测,品牌供应,我们拥有三千多种酶联免疫吸附测定试剂盒,包含多种种属ELISA试剂盒,待测标本形式多样:有液体及半固体形式。
样本保存:
2-8℃冰箱最多2周
-20到-50℃的冰箱可以放3-6个月
人抗类固醇生成细胞抗体ELISA试剂盒厂家-50℃一下的冰箱,可以放一年
样本收集:
血清样本用干燥管(红色)或者促凝管(黄色)收集
血浆用抗凝管,一般有EDTA抗凝(紫色)、枸橼酸钠抗凝(黑色或者蓝色)
血浆样本最好是不要用肝素(绿色)抗凝的试管收集
样本处理:
1)血清:室温血液自然凝固10-20分钟,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如出现沉淀,应再次离心。
2)血浆:应根据标本的要求选择EDTA或柠檬酸钠作为抗凝剂,混合10-20分钟后,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如有沉淀形成,应该再次离心。
3)尿液:用无菌管收集,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如有沉淀形成,应再次离心。胸腹水、脑脊液参照实行。
4)细胞培养上清:检测分泌性的成份时,用无菌管收集。离心20分钟左右(2000-3000转/分)。仔细收集上清。检测细胞内的成份时,用PBS(PH7.2-7.4)稀释细胞悬液,细胞浓度达到100万/ml左右。人抗类固醇生成细胞抗体ELISA试剂盒厂家通过反复冻融,以使细胞破坏并放出细胞内成份。离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。
5)组织标本:切割标本后,称取重量。加入一定量的PBS,PH7.4。用液氮迅速冷冻保存备用。标本融化后仍然保持2-8℃的温度。加入一定量的PBS(PH7.4),用手工或匀浆器将标本匀浆充分。离心20分钟左右(2000-3000转/分)。仔细收集上清。分装后一份待检测,其余冷冻备用。
6)标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验。若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融.
7)不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的(HRP)活性。
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EIA(B004)-031292 人甾醇-O-酰基转移酶2(SOAT2)ELISA Kit ,Products name: Human SOAT2 ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
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EIA(B004)-031295 人载脂蛋白A1(APOA1)ELISA Kit ,Products name: APOA1 ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-031296 人载脂蛋白A1(apo-A1)ELISA Kit ,Products name: Human apoprotein A1,apo-A1 ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-031297 人载脂蛋白A1(apo-A1)ELISA Kit ,Products name: Human apoprotein A1,apo-A1 ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-031298 人载脂蛋白A1(apo-A1)ELISA Kit ,Products name: Human apo-A1 ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-031299 人载脂蛋白A1(apo-A1)ELISA Kit ,Products name: Apo-A1 ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-031300 人载脂蛋白A1前体(Pre-Apo-A1)ELISA Kit ,Products name: Pre-APO-A1 ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
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EIA(B004)-031302 人载脂蛋白A2(APOA2)ELISA Kit ,Products name: APOA2 ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-031303 人载脂蛋白A2(apo-A2)ELISA Kit ,Products name: Human apo-A2 ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-031304 人载脂蛋白A4(APOA4)ELISA Kit ,Products name: Human APOA4 ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-031305 人载脂蛋白A4(APOA4)ELISA Kit ,Products name: APOA4 ELISA Kit ,灵敏度: 0.094ng/mL ,检测范围: 0.156-10ng/mL
EIA(B004)-031306 人载脂蛋白A4(Apo-A4)ELISA Kit ,Products name: Human Apo-A4 ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-031307 人载脂蛋白A5(apoA5)ELISA Kit ,Products name: apoA5 ELISA Kit ,灵敏度: 0.094ng/mL ,检测范围: 0.156-10ng/mL
EIA(B004)-031308 人载脂蛋白A5(APOA5)ELISA Kit ,Products name: Human APOA5 ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-031309 人载脂蛋白A5(APOA5)ELISA Kit ,Products name: APOA5 ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-031310 人载脂蛋白A5(apo-A5)ELISA Kit ,Products name: Human apoprotein A5,apo-A5 ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-031311 人载脂蛋白A5(apo-A5)ELISA Kit ,Products name: Human apoprotein A5,apo-A5 ELISA Kit ,灵敏度: 0.094ng/mL ,检测范围: 0.156-10ng/mL
EIA(B004)-031312 人载脂蛋白A5(apo-A5)ELISA Kit ,Products name: Human apo-A5 ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
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EIA(B004)-031314 人载脂蛋白B(APOB)ELISA Kit ,Products name: Human APOB ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
EIA(B004)-031315 人载脂蛋白B(APOB)ELISA Kit ,Products name: APOB ELISA Kit ,灵敏度: 18.75pg/mL ,检测范围: 31.25-2000pg/mL
EIA(B004)-031316 人载脂蛋白B100(APOB100)ELISA Kit ,Products name: Human APOB100 ELISA Kit ,灵敏度: 9.375pg/mL ,检测范围: 15.625-1000pg/mL
EIA(B004)-031317 人载脂蛋白B100(apo-B100)ELISA Kit ,Products name: Human apoprotein B100,apo-B100 ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
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EIA(B004)-031342 人载脂蛋白E(Apo-E)ELISA Kit ,Products name: Human Apolipoprotein E,Apo-E ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
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EIA(B004)-031344 人载脂蛋白E(Apo-E)ELISA Kit ,Products name: Human Apo-E ELISA Kit ,灵敏度: 0pg/mL ,检测范围: 0pg/mL
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EIA(B004)-031346 人载脂蛋白F(APOF)ELISA Kit ,Products name: APOF ELISA Kit ,灵敏度: 0.094ng/mL ,检测范围: 0.156-10ng/mL
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