?磷酸化肌球蛋白轻链9抗体
『别名』20 kDa myosin light chain; Human 20kDa myosin light chain (MLC2) mRNA complete cds; LC20; MGC3505; MLC 2; MLC-2C; MLC2; MLY 9; MRLC1; MYL9; MYL9_HUMAN; Myosin light chain 9 regulatory; Myosin light polypeptide 9 regulatory; Myosin regulatory light chain 2; Myosin regulatory light chain 2 smooth muscle isoform; Myosin regulatory light chain 9; Myosin regulatory light chain MRLC1; Myosin regulatory light polypeptide 9; Myosin RLC; Myosin vascular smooth muscle light chain 2; MYRL2; OTTHUMP00000030857; smooth muscle isoform.
『浓度』磷酸化肌球蛋白轻链9抗体1mg/1ml
『规格』0.1ml/100μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
磷酸化肌球蛋白轻链9抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
磷酸化肌球蛋白轻链9抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
PAA734Mu02 蛋白C(PROC)多克隆抗体 Polyclonal Antibody to Protein C (PROC)
PAK108Hu01 端粒保护1同源物(POT1)多克隆抗体 Polyclonal Antibody to Protection Of Telomeres 1 Homolog (POT1)
PAB336Hu01 保护素(CD59)多克隆抗体 Polyclonal Antibody to Protectin (CD59)
PAB336Mu01 保护素(CD59)多克隆抗体 Polyclonal Antibody to Protectin (CD59)
PAB336Ra01 保护素(CD59)多克隆抗体 Polyclonal Antibody to Protectin (CD59)
PAB336Rb51 保护素(CD59)多克隆抗体 Polyclonal Antibody to Protectin (CD59)
PAB336Si01 保护素(CD59)多克隆抗体 Polyclonal Antibody to Protectin (CD59)
PAB336Po01 保护素(CD59)多克隆抗体 Polyclonal Antibody to Protectin (CD59)
PAB336Mu02 保护素(CD59)多克隆抗体 Polyclonal Antibody to Protectin (CD59)
PAG273Hu01 蛋白酶体亚基β6(PSMβ6)多克隆抗体 Polyclonal Antibody to Proteasome Subunit Beta Type 6 (PSMb6)
PAG262Hu01 蛋白酶体亚基α7(PSMα7)多克隆抗体 Polyclonal Antibody to Proteasome Subunit Alpha Type 7 (PSMa7)
PAG260Hu01 蛋白酶体亚基α5(PSMα5)多克隆抗体 Polyclonal Antibody to Proteasome Subunit Alpha Type 5 (PSMa5)
PAD329Hu01 蛋白酶体亚基α1(PSMα1)多克隆抗体 Polyclonal Antibody to Proteasome Subunit Alpha Type 1 (PSMa1)
PAG283Hu01 蛋白酶激活亚基3(PSME3)多克隆抗体 Polyclonal Antibody to Proteasome Activator Subunit 3 (PSME3)
PAP779Hu01 非ATP酶蛋白酶体26S亚基9(PSMD9)多克隆抗体 Polyclonal Antibody to Proteasome 26S Subunit, Non ATPase 9 (PSMD9)
PAG282Hu01 非ATP酶蛋白酶体26S亚基7(PSMD7)多克隆抗体 Polyclonal Antibody to Proteasome 26S Subunit, Non ATPase 7 (PSMD7)
PAQ311Hu01 非ATP酶蛋白酶体26S亚基6(PSMD6)多克隆抗体 Polyclonal Antibody to Proteasome 26S Subunit, Non ATPase 6 (PSMD6)
PAG281Hu01 非ATP酶蛋白酶体26S亚基5(PSMD5)多克隆抗体 Polyclonal Antibody to Proteasome 26S Subunit, Non ATPase 5 (PSMD5)
PAG280Hu01 非ATP酶蛋白酶体26S亚基4(PSMD4)多克隆抗体 Polyclonal Antibody to Proteasome 26S Subunit, Non ATPase 4 (PSMD4)
PAG279Hu01 非ATP酶蛋白酶体26S亚基2(PSMD2)多克隆抗体 Polyclonal Antibody to Proteasome 26S Subunit, Non ATPase 2 (PSMD2)
PAP780Hu01 非ATP酶蛋白酶体26S亚基13(PSMD13)多克隆抗体 Polyclonal Antibody to Proteasome 26S Subunit, Non ATPase 13 (PSMD13)
PAP783Hu01 非ATP酶蛋白酶体26S亚基10(PSMD10)多克隆抗体 Polyclonal Antibody to Proteasome 26S Subunit, Non ATPase 10 (PSMD10)
PAG276Hu01 ATP酶蛋白酶体26S亚基6(PSMC6)多克隆抗体 Polyclonal Antibody to Proteasome 26S Subunit, ATPase 6 (PSMC6)
PAG274Hu01 ATP酶蛋白酶体26S亚基4(PSMC4)多克隆抗体 Polyclonal Antibody to Proteasome 26S Subunit, ATPase 4 (PSMC4)
PAG278Hu01 ATP酶蛋白酶体26S亚基3(PSMC3)多克隆抗体 Polyclonal Antibody to Proteasome 26S Subunit, ATPase 3 (PSMC3)
PAG277Hu01 ATP酶蛋白酶体26S亚基2(PSMC2)多克隆抗体 Polyclonal Antibody to Proteasome 26S Subunit, ATPase 2 (PSMC2)
PAD327Hu01 ATP酶蛋白酶体26S亚基1(PSMC1)多克隆抗体 Polyclonal Antibody to Proteasome 26S Subunit, ATPase 1 (PSMC1)
PAG321Hu01 丝氨酸蛋白酶8(PRSS8)多克隆抗体 Polyclonal Antibody to Protease, Serine 8 (PRSS8)
PAG321Ra01 丝氨酸蛋白酶8(PRSS8)多克隆抗体 Polyclonal Antibody to Protease, Serine 8 (PRSS8)
PAG321Ra02 丝氨酸蛋白酶8(PRSS8)多克隆抗体 Polyclonal Antibody to Protease, Serine 8 (PRSS8)
PAG321Mu01 丝氨酸蛋白酶8(PRSS8)多克隆抗体 Polyclonal Antibody to Protease, Serine 8 (PRSS8)
PAN806Hu01 丝氨酸蛋白酶50(PRSS50)多克隆抗体 Polyclonal Antibody to Protease, Serine 50 (PRSS50)
PAS152Hu01 丝氨酸蛋白酶33(PRSS33)多克隆抗体 Polyclonal Antibody to Protease, Serine 33 (PRSS33)
PAQ421Hu01 丝氨酸蛋白酶23(PRSS23)多克隆抗体 Polyclonal Antibody to Protease, Serine 23 (PRSS23)
PAB537Hu01 丝氨酸蛋白酶2(PRSS2)多克隆抗体 Polyclonal Antibody to Protease, Serine 2 (PRSS2)
PAB537Mu01 丝氨酸蛋白酶2(PRSS2)多克隆抗体 Polyclonal Antibody to Protease, Serine 2 (PRSS2)
PAB400Mu01 丝氨酸蛋白酶12(PRSS12)多克隆抗体 Polyclonal Antibody to Protease, Serine 12 (PRSS12)
PAB400Hu01 丝氨酸蛋白酶12(PRSS12)多克隆抗体 Polyclonal Antibody to Protease, Serine 12 (PRSS12)
PAA230Hu01 丝氨酸蛋白酶1(PRSS1)多克隆抗体 Polyclonal Antibody to Protease, Serine 1 (PRSS1)
PAA230Ra01 丝氨酸蛋白酶1(PRSS1)多克隆抗体 Polyclonal Antibody to Protease, Serine 1 (PRSS1)
PAA127Hu01 蛋白酶激活受体4(PAR4)多克隆抗体 Polyclonal Antibody to Protease Activated Receptor 4 (PAR4)
PAA127Ra01 蛋白酶激活受体4(PAR4)多克隆抗体 Polyclonal Antibody to Protease Activated Receptor 4 (PAR4)
PAA852Hu01 蛋白酶激活受体2(PAR2)多克隆抗体 Polyclonal Antibody to Protease Activated Receptor 2 (PAR2)
PAA852Mu01 蛋白酶激活受体2(PAR2)多克隆抗体 Polyclonal Antibody to Protease Activated Receptor 2 (PAR2)
PAA852Ra01 蛋白酶激活受体2(PAR2)多克隆抗体 Polyclonal Antibody to Protease Activated Receptor 2 (PAR2)
PAA852Mu02 蛋白酶激活受体2(PAR2)多克隆抗体 Polyclonal Antibody to Protease Activated Receptor 2 (PAR2)
PAC939Hu01 蛋白酶激活受体1(PAR1)多克隆抗体 Polyclonal Antibody to Protease Activated Receptor 1 (PAR1)
PAC939Ra01 蛋白酶激活受体1(PAR1)多克隆抗体 Polyclonal Antibody to Protease Activated Receptor 1 (PAR1)
PAH307Hu01 鱼精蛋白2(PRM2)多克隆抗体 Polyclonal Antibody to Protamine 2 (PRM2)
PAH307Mu01 鱼精蛋白2(PRM2)多克隆抗体 Polyclonal Antibody to Protamine 2 (PRM2)
PAH308Hu01 鱼精蛋白1(PRM1)多克隆抗体 Polyclonal Antibody to Protamine 1 (PRM1)
PAC735Hu01 前列腺干细胞抗原(PSCA)多克隆抗体 Polyclonal Antibody to Prostate Stem Cell Antigen (PSCA)
PAC735Hu02 前列腺干细胞抗原(PSCA)多克隆抗体 Polyclonal Antibody to Prostate Stem Cell Antigen磷酸化肌球蛋白轻链9抗体 (PSCA)
PAA740Hu01 前列腺素I合酶(PTGIS)多克隆抗体 Polyclonal Antibody to Prostaglandin I Synthase (PTGIS)
PAA699Hu01 环加氧酶2(PTGS2)多克隆抗体 Polyclonal Antibody to Prostaglandin Endoperoxide Synthase 2 (PTGS2)
PAA699Mu01 环加氧酶2(PTGS2)多克隆抗体 Polyclonal Antibody to Prostaglandin Endoperoxide Synthase 2 (PTGS2)