环指蛋白59抗体
『别名』BBBG 1; BBBG1; Finger on X and Y mouse homolog of antibody; FXY; GBBB 1; GBBB1; MID 1; MID-1; Mid1; Midin; Midline 1 (Opitz/BBB syndrome); Midline 1; Midline 1 ring finger; Midline 1 RING finger protein; Midline-1; Midline1; OGS 1; OGS1; OS antibody; OSX; Putative transcription factor XPRF; RING finger protein 59; RNF 59; RNF59; TRI18; TRI18_HUMAN; TRIM 18; TRIM18; Tripartite motif containing protein 18; Tripartite motif protein TRIM18; Tripartite motif-containing protein 18; XPRF; Zinc finger X and Y antibody; ZNFXY.
『浓度』环指蛋白59抗体1mg/1ml
『规格』0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
环指蛋白59抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
环指蛋白59抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
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PAE676Mu01 再生胰岛衍生蛋白3γ(REG3γ)多克隆抗体 Polyclonal Antibody to Regenerating Islet Derived Protein 3 Gamma (REG3g)
PAE676Ra01 再生胰岛衍生蛋白3γ(REG3γ)多克隆抗体 Polyclonal Antibody to Regenerating Islet Derived Protein 3 Gamma (REG3g)
PAE676Mu02 再生胰岛衍生蛋白3γ(REG3γ)多克隆抗体 Polyclonal Antibody to Regenerating Islet Derived Protein 3 Gamma (REG3g)
PAK541Mu01 再生胰岛衍生蛋白3β(REG3β)多克隆抗体 Polyclonal Antibody to Regenerating Islet Derived Protein 3 Beta (REG3b)
PAK541Mu02 再生胰岛衍生蛋白3β(REG3β)多克隆抗体 Polyclonal Antibody to Regenerating Islet Derived Protein 3 Beta (REG3b)
PAK541Ra01 再生胰岛衍生蛋白3β(REG3β)多克隆抗体 Polyclonal Antibody to Regenerating Islet Derived Protein 3 Beta (REG3b)
PAE675Hu01 再生胰岛衍生蛋白3α(REG3α)多克隆抗体 Polyclonal Antibody to Regenerating Islet Derived Protein 3 Alpha (REG3a)
PAE675Ra01 再生胰岛衍生蛋白3α(REG3α)多克隆抗体 Polyclonal Antibody to Regenerating Islet Derived Protein 3 Alpha (REG3a)
PAE675Mu01 再生胰岛衍生蛋白3α(REG3α)多克隆抗体 Polyclonal Antibody to Regenerating Islet Derived Protein 3 Alpha (REG3a)
PAE675Mu02 再生胰岛衍生蛋白3α(REG3α)多克隆抗体 Polyclonal Antibody to Regenerating Islet Derived Protein 3 Alpha (REG3a)
PAE674Hu01 再生胰岛衍生蛋白1β(REG1β)多克隆抗体 Polyclonal Antibody to Regenerating Islet Derived Protein 1 Beta (REG1b)
PAE674Mu01 再生胰岛衍生蛋白1β(REG1β)多克隆抗体 Polyclonal Antibody to Regenerating Islet Derived Protein 1 Beta (REG1b)
PAE674Mu02 再生胰岛衍生蛋白1β(REG1β)多克隆抗体 Polyclonal Antibody to Regenerating Islet Derived Protein 1 Beta (REG1b)
PAB760Hu01 再生胰岛衍生蛋白1α(REG1α)多克隆抗体 Polyclonal Antibody to Regenerating Islet Derived Protein 1 Alpha (REG1a)
PAB760Ra01 再生胰岛衍生蛋白1α(REG1α)多克隆抗体 Polyclonal Antibody to Regenerating Islet Derived Protein 1 Alpha (REG1a)
PAB760Mu01 再生胰岛衍生蛋白1α(REG1α)多克隆抗体 Polyclonal Antibody to Regenerating Islet Derived Protein 1 Alpha (REG1a)
PAB760Mu02 再生胰岛衍生蛋白1α(REG1α)多克隆抗体 Polyclonal Antibody to Regenerating Islet Derived Protein 1 Alpha (REG1a)
PAC775Hu01 络丝蛋白(RL)多克隆抗体 Polyclonal Antibody to Reelin (RL)
PAC775Mu01 络丝蛋白(RL)多克隆抗体 Polyclonal Antibody to Reelin (RL)
PAC775Ra01 络丝蛋白(RL)多克隆抗体 Polyclonal Antibody to Reelin (RL)
PAC775Mu02 络丝蛋白(RL)多克隆抗体 Polyclonal Antibody to Reelin (RL)
PAC775Hu03 络丝蛋白(RL)多克隆抗体 Polyclonal Antibody to Reelin (RL)
PAC775Hu02 络丝蛋白(RL)多克隆抗体 Polyclonal Antibody to Reelin (RL)
PAC775Hu04 络丝蛋白(RL)多克隆抗体 Polyclonal Antibody to Reelin (RL)
PAF484Hu01 恢复蛋白(RCVRN)多克隆抗体 Polyclonal Antibody to Recoverin (RCVRN)
PAE272Hu01 受体酪氨酸激酶样孤儿素受体2(ROR2)多克隆抗体 Polyclonal Antibody to Receptor Tyrosine Kinase Like Orphan Receptor 2 (ROR2)
PAG522Hu01 受体酪氨酸激酶样孤儿素受体1(ROR1)多克隆抗体 Polyclonal Antibody to Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1)
PAG522Mu01 受体酪氨酸激酶样孤儿素受体1(ROR1)多克隆抗体 Polyclonal Antibody to Receptor Tyrosine Kinase Like Orphan Receptor 1 (ROR1)
PAE639Ra01 受体相互作用丝氨酸苏氨酸激酶3(RIPK3)多克隆抗体 Polyclonal Antibody to Receptor Interacting Serine Threonine Kinase 3 (RIPK3)
PAE639Mu01 受体相互作用丝氨酸苏氨酸激酶3(RIPK3)多克隆抗体 Polyclonal Antibody to Receptor Interacting Serine Threonine Kinase 3 (RIPK3)
PAE639Hu01 受体相互作用丝氨酸苏氨酸激酶3(RIPK3)多克隆抗体 Polyclonal Antibody to Receptor Interacting Serine Threonine Kinase 3 (RIPK3)
PAE640Hu01 受体相互作用丝氨酸苏氨酸激酶1(RIPK1)多克隆抗体 Polyclonal Antibody to Receptor Interacting Serine Threonine Kinase 1 (RIPK1)
PAE640Ra01 受体相互作用丝氨酸苏氨酸激酶1(RIPK1)多克隆抗体 Polyclonal Antibody to Receptor Interacting Serine Threonine Kinase 1 (RIPK1)
PAB579Hu01 IgG-Fc片段受体Ⅱ(FcγRⅡ)多克隆抗体 Polyclonal Antibody to Receptor II For The Fc Region Of Immunoglobulin G (FcgRII)
PAB579Ra01 IgG-Fc片段受体Ⅱ(FcγRⅡ)多克隆抗体 Polyclonal Antibody to Receptor II For The Fc Region Of Immunoglobulin G (FcgRII)
PAB582Hu01 IgE-Fc片段受体Ⅱ(FcεRⅡ)多克隆抗体 Polyclonal Antibody to Receptor II For The Fc Region Of Immunoglobulin E (FceRII)
PAB582Mu01 IgE-Fc片段受体Ⅱ(FcεRⅡ)多克隆抗体 Polyclonal Antibody to Receptor II For The Fc Region Of Immunoglobulin E (FceRII)
PAB582Mu02 IgE-Fc片段受体Ⅱ(FcεRⅡ)多克隆抗体 Polyclonal Antibody to Receptor II For The Fc Region Of Immunoglobulin E (FceRII)
PAB578Hu01 IgG-Fc片段受体Ⅰ(FcγRⅠ)多克隆抗体 Polyclonal Antibody to Receptor I For The Fc Region Of Immunoglobulin G (FcgRI)
PAB578Mu01 IgG-Fc片段受体Ⅰ(FcγRⅠ)多克隆抗体 Polyclonal Antibody to Receptor I For The Fc Region Of Immunoglobulin G (FcgRI)
PAB578Mu02 IgG-Fc片段受体Ⅰ(FcγRⅠ)多克隆抗体 Polyclonal Antibody to Receptor I For The Fc Region Of Immunoglobulin G (FcgRI)
PAB581Mu01 IgE-Fc受体Ⅰ(FcεRⅠ)多克隆抗体 Polyclonal Antibody to Receptor I For The Fc Region Of Immunoglobulin E (FceRI)
PAB581Mu02 IgE-Fc受体Ⅰ(FcεRⅠ)多克隆抗体 Polyclonal Antibody to Receptor I For The Fc Region Of Immunoglobulin E (FceRI)
PAC762Mu01 突触受体关联蛋白(RAPSN)多克隆抗体 Polyclonal Antibody to Receptor Associated Protein Of The Synapse (RAPSN)
PAC762Mu02 突触受体关联蛋白(RAPSN)多克隆抗体 Polyclonal Antibody to Receptor Associated Protein Of The Synapse (RAPSN)
PAH262Hu01 受体活性修饰蛋白2(RAMP2)多克隆抗体 Polyclonal Antibody to Receptor Activity Modifying Protein 2 (RAMP2)
PAA855Ga01 核因子κB受体激活因子配基(RANκL)多克隆抗体 Polyclonal Antibody to Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)
PAA855Hu01 核因子κB受体激活因子配基(RANκL)多克隆抗体 Polyclonal Antibody to Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)
PAA855Mu01 核因子κB受体激活因子配基(RANκL)多克隆抗体 Polyclonal Antibody to Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)
PAA855Ra01 核因子κB受体激活因子配基(RANκL)多克隆抗体 Polyclonal Antibody to Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)
PAA855Mu02 核因子κB受体激活因子配基(RANκL)多克隆抗体 Polyclonal Antibody to Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)
PAC057Hu01 核因子κB受体激活因子(RANκ)多克隆抗体 Polyclonal Antibody to Receptor Activator Of Nuclear Factor Kappa B (RANk)
PAC057Ra01 核因子κB受体激活因子(RANκ)多克隆抗体 Polyclonal Antibody to Receptor Activator Of Nuclear Factor Kappa B (RANk)
PAC057Mu01 核因子κB受体激活因子(RANκ)多克隆抗体 Polyclonal Antibody to Receptor Activator Of Nuclear Factor Kappa B (RANk)
PAQ510Hu01 活性氧物种调节因子1(ROMO1)多克隆抗体 Polyclonal Antibody to Reactive Oxygen Species Modulator 1 (ROMO1)
PAM113Hu01 含RCC1和BTB域蛋白2环指蛋白59抗体(RCBTB2)多克隆抗体 Polyclonal Antibody to RCC1 And BTB Domain Containing Protein 2 (RCBTB2)
PAM427Ra01 Ras相关C3肉毒菌毒素底物1(Rac1)多克隆抗体 Polyclonal Antibody to Ras Related C3 Botulinum Toxin Substrate 1 (Rac1)
PAM427Hu01 Ras相关C3肉毒菌毒素底物1(Rac1)多克隆抗体 Polyclonal Antibody to Ras Related C3 Botulinum Toxin Substrate 1 (Rac1)
PAE328Hu01 Ras同源基因家族成员Q(RHOQ)多克隆抗体 Polyclonal Antibody to Ras Homolog Gene Family, Member Q (RHOQ)