甲硝唑单克隆抗体
『别名』2-Methyl-5-nitroimidazole-1-ethanol
『浓度』甲硝唑单克隆抗体1mg/1ml
『规格』0.1ml/100μg 0.2ml/200μg
『抗体来源』Mouse
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
甲硝唑单克隆抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
甲硝唑单克隆抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
PAN346Ra02 Reprimo蛋白(RPRM)多克隆抗体 Polyclonal Antibody to Reprimo (RPRM)
PAH217Hu01 复制蛋白A1(RPA1)多克隆抗体 Polyclonal Antibody to Replication Protein A1 (RPA1)
PAH217Ra01 复制蛋白A1(RPA1)多克隆抗体 Polyclonal Antibody to Replication Protein A1 (RPA1)
PAG516Hu01 复制启动因子1(REPIN1)多克隆抗体 Polyclonal Antibody to Replication Initiator 1 (REPIN1)
PAG516Mu01 复制启动因子1(REPIN1)多克隆抗体 Polyclonal Antibody to Replication Initiator 1 (REPIN1)
PAG516Mu02 复制启动因子1(REPIN1)多克隆抗体 Polyclonal Antibody to Replication Initiator 1 (REPIN1)
PAC764Hu01 肾素结合蛋白(RENBP)多克隆抗体 Polyclonal Antibody to Renin Binding Protein (RENBP)
PAA889Ca01 肾素(REN)多克隆抗体 Polyclonal Antibody to Renin (REN)
PAA889Hu01 肾素(REN)多克隆抗体 Polyclonal Antibody to Renin (REN)
PAA889Mu01 肾素(REN)多克隆抗体 Polyclonal Antibody to Renin (REN)
PAA889Ra01 肾素(REN)多克隆抗体 Polyclonal Antibody to Renin (REN)
PAA889Rb51 肾素(REN)多克隆抗体 Polyclonal Antibody to Renin (REN)
PAA889Mu02 肾素(REN)多克隆抗体 Polyclonal Antibody to Renin (REN)
PAA889Ra02 肾素(REN)多克隆抗体 Polyclonal Antibody to Renin (REN)
PAC845Hu01 肾酶(RNLS)多克隆抗体 Polyclonal Antibody to Renalase (RNLS)
PAC845Mu01 肾酶(RNLS)多克隆抗体 Polyclonal Antibody to Renalase (RNLS)
PAC845Mu02 肾酶(RNLS)多克隆抗体 Polyclonal Antibody to Renalase (RNLS)
PAC845Hu02 肾酶(RNLS)多克隆抗体 Polyclonal Antibody to Renalase (RNLS)
PAC845Ra01 肾酶(RNLS)多克隆抗体 Polyclonal Antibody to Renalase (RNLS)
PAH266Hu01 肾肿瘤抗原(RAGE)多克隆抗体 Polyclonal Antibody to Renal Tumor Antigen (RAGE)
PAH266Ra01 肾肿瘤抗原(RAGE)多克隆抗体 Polyclonal Antibody to Renal Tumor Antigen (RAGE)
PAH266Mu01 肾肿瘤抗原(RAGE)多克隆抗体 Polyclonal Antibody to Renal Tumor Antigen (RAGE)
PAD866Hu01 松弛素/胰岛素样肽受体3(RXFP3)多克隆抗体 Polyclonal Antibody to Relaxin/Insulin Like Family Peptide Receptor 3 (RXFP3)
PAB456Hu01 松弛素/胰岛素样肽受体1(RXFP1)多克隆抗体 Polyclonal Antibody to Relaxin/Insulin Like Family Peptide Receptor 1 (RXFP1)
PAB456Hu02 松弛素/胰岛素样肽受体1(RXFP1)多克隆抗体 Polyclonal Antibody to Relaxin/Insulin Like Family Peptide Receptor 1 (RXFP1)
PAD869Ra01 松弛肽3(RLN3)多克隆抗体 Polyclonal Antibody to Relaxin 3 (RLN3)
PAD869Mu01 松弛肽3(RLN3)多克隆抗体 Polyclonal Antibody to Relaxin 3 (RLN3)
PAD869Mu02 松弛肽3(RLN3)多克隆抗体 Polyclonal Antibody to Relaxin 3 (RLN3)
PAD868Hu01 松弛肽2(RLN2)多克隆抗体 Polyclonal Antibody to Relaxin 2 (RLN2)
PAD868Ra01 松弛肽2(RLN2)多克隆抗体 Polyclonal Antibody to Relaxin 2 (RLN2)
PAB216Bo01 松弛肽(RLN)多克隆抗体 Polyclonal Antibody to Relaxin (RLN)
PAB216Hu01 松弛肽(RLN)多克隆抗体 Polyclonal Antibody to Relaxin (RLN)
PAB216Mu01 松弛肽(RLN)多克隆抗体 Polyclonal Antibody to Relaxin (RLN)
PAB216Po01 松弛肽(RLN)多克隆抗体 Polyclonal Antibody to Relaxin (RLN)
PAB216Mu02 松弛肽(RLN)多克隆抗体 Polyclonal Antibody to Relaxin (RLN)
PAB216Ra01 松弛肽(RLN)多克隆抗体 Polyclonal Antibody to Relaxin (RLN)
PAB216Rb51 松弛肽(RLN)多克隆抗体 Polyclonal Antibody to Relaxin (RLN)
PAM129Mu01 相关受体酪氨酸激酶(RYK)多克隆抗体 Polyclonal Antibody to Related To Receptor Tyrosine Kinase (RYK)
PAM129Hu01 相关受体酪氨酸激酶(RYK)多克隆抗体 Polyclonal Antibody to Related To Receptor Tyrosine Kinase (RYK)
PAD706Ra01 蛋白信号调节因子6(RGS6)多克隆抗体 Polyclonal Antibody to Regulator Of G Protein Signaling 6 (RGS6)
PAD705Mu01 蛋白信号调节因子5(RGS5)多克隆抗体 Polyclonal Antibody to Regulator Of G Protein Signaling 5 (RGS5)
PAD705Mu02 蛋白信号调节因子5(RGS5)多克隆抗体 Polyclonal Antibody to Regulator Of G Protein Signaling 5 (RGS5)
PAA978Hu01 蛋白信号调节因子4(RGS4)多克隆抗体 Polyclonal Antibody to Regulator Of G Protein Signaling 4 (RGS4)
PAP367Hu01 蛋白信号调节因子19(RGS19)多克隆抗体 Polyclonal Antibody to Regulator Of G Protein Signaling 19 (RGS19)
PAD701Hu01 蛋白信号调节因子1(RGS1)多克隆抗体 Polyclonal Antibody to Regulator Of G Protein Signaling 1 (RGS1)
PAA116Hu01 调节激活正常T-细胞表达分泌因子(RANTES)多克隆抗体 Polyclonal Antibody to Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES)
PAA116Mu01 调节激活正常T-细胞表达分泌因子(RANTES)多克隆抗体 Polyclonal Antibody to Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES)
PAA116Ra01 调节激活正常T-细胞表达分泌因子(RANTES)多克隆抗体 Polyclonal Antibody to Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES)
PAA116Gu01 调节激活正常T-细胞表达分泌因子(RANTES)多克隆抗体 Polyclonal Antibody to Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES)
PAA116Si01 调节激活正常T-细胞表达分泌因子(RANTES)多克隆抗体 Polyclonal Antibody to Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES)
PAA116Ca01 调节激活正常T-细胞表达分泌因子(RANTES)多克隆抗体 Polyclonal Antibody to Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES)
PAA116Po01 调节激活正常T-细胞表达分泌因子(RANTES)多克隆抗体 Polyclonal Antibody to Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES)
PAA116Bo01 调节激活正常T-细胞表达分泌因子(RANTES)多克隆抗体 Polyclonal Antibody to Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES)
PAA116Mu02 调节激活正常T-细胞表达分泌因子(RANTES)多克隆抗体 Polyclonal Antibody to Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES)
PAA116Eq01 甲硝唑单克隆抗体调节激活正常T-细胞表达分泌因子(RANTES)多克隆抗体 Polyclonal Antibody to Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES)
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PAB819Hu01 再生胰岛衍生蛋白4(REG4)多克隆抗体 Polyclonal Antibody to Regenerating Islet Derived Protein 4 (REG4)
PAB819Mu01 再生胰岛衍生蛋白4(REG4)多克隆抗体 Polyclonal Antibody to Regenerating Islet Derived Protein 4 (REG4)