微管相关蛋白4抗体
『别名』DKFZp779A1753; MAP-4; MAP4; MAP4_HUMAN; MGC8617; Microtubule associated protein 4; Microtubule-associated protein 4; OTTHUMP00000210723; OTTHUMP00000210725; OTTHUMP00000210727; OTTHUMP00000210730.
『浓度』微管相关蛋白4抗体1mg/1ml
『规格』0.1ml/100μg 0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
微管相关蛋白4抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
微管相关蛋白4抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
PAE945Hu01 环指蛋白39(RNF39)多克隆抗体 Polyclonal Antibody to Ring Finger Protein 39 (RNF39)
PAS096Pl01 核酮糖-1,5-二磷酸羧化酶/加氧酶(RuBisCO)多克隆抗体 Polyclonal Antibody to Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase (RuBisCO)
PAM086Hu01 核糖体蛋白S6激酶β2(RPS6Kβ2)多克隆抗体 Polyclonal Antibody to Ribosomal Protein S6 Kinase Beta 2 (RPS6Kb2)
PAL979Hu01 核糖体蛋白S6激酶β1(RPS6Kβ1)多克隆抗体 Polyclonal Antibody to Ribosomal Protein S6 Kinase Beta 1 (RPS6Kb1)
PAM085Hu01 核糖体蛋白S6激酶α1(RPS6Kα1)多克隆抗体 Polyclonal Antibody to Ribosomal Protein S6 Kinase Alpha 1 (RPS6Ka1)
PAF046Hu01 核糖体蛋白L6(RPL6)多克隆抗体 Polyclonal Antibody to Ribosomal Protein L6 (RPL6)
PAP643Hu01 核糖体蛋白L23A(RPL23A)多克隆抗体 Polyclonal Antibody to Ribosomal Protein L23A (RPL23A)
PAQ321Hu01 核糖体蛋白L13A(RPL13A)多克隆抗体 Polyclonal Antibody to Ribosomal Protein L13A (RPL13A)
PAC769Hu01 核糖体结合糖蛋白Ⅰ(RPN1)多克隆抗体 Polyclonal Antibody to Ribophorin I (RPN1)
PAC769Hu02 核糖体结合糖蛋白Ⅰ(RPN1)多克隆抗体 Polyclonal Antibody to Ribophorin I (RPN1)
PAC769Hu04 核糖体结合糖蛋白Ⅰ(RPN1)多克隆抗体 Polyclonal Antibody to Ribophorin I (RPN1)
PAC769Hu03 核糖体结合糖蛋白Ⅰ(RPN1)多克隆抗体 Polyclonal Antibody to Ribophorin I (RPN1)
PAC769Mu01 核糖体结合糖蛋白Ⅰ(RPN1)多克隆抗体 Polyclonal Antibody to Ribophorin I (RPN1)
PAF914Hu01 核苷酸还原酶M1(RRM1)多克隆抗体 Polyclonal Antibody to Ribonucleotide Reductase M1 (RRM1)
PAF914Mu01 核苷酸还原酶M1(RRM1)多克隆抗体 Polyclonal Antibody to Ribonucleotide Reductase M1 (RRM1)
PAF914Mu02 核苷酸还原酶M1(RRM1)多克隆抗体 Polyclonal Antibody to Ribonucleotide Reductase M1 (RRM1)
PAA113Hu01 核糖核酸酶T2(RNASET2)多克隆抗体 Polyclonal Antibody to Ribonuclease T2 (RNASET2)
PAA113Mu01 核糖核酸酶T2(RNASET2)多克隆抗体 Polyclonal Antibody to Ribonuclease T2 (RNASET2)
PAA113Ra01 核糖核酸酶T2(RNASET2)多克隆抗体 Polyclonal Antibody to Ribonuclease T2 (RNASET2)
PAA113Mu02 核糖核酸酶T2(RNASET2)多克隆抗体 Polyclonal Antibody to Ribonuclease T2 (RNASET2)
PAA198Ra01 核糖核酸酶P(RNASEP)多克隆抗体 Polyclonal Antibody to Ribonuclease P (RNASEP)
PAA198Hu01 核糖核酸酶P(RNASEP)多克隆抗体 Polyclonal Antibody to Ribonuclease P (RNASEP)
PAD164Hu01 核糖核酸酶L(RNASEL)多克隆抗体 Polyclonal Antibody to Ribonuclease L (RNASEL)
PAD164Hu02 核糖核酸酶L(RNASEL)多克隆抗体 Polyclonal Antibody to Ribonuclease L (RNASEL)
PAB444Hu01 核糖核酸抑制因子(RI)多克隆抗体 Polyclonal Antibody to Ribonuclease Inhibitor (RI)
PAB444Ra01 核糖核酸抑制因子(RI)多克隆抗体 Polyclonal Antibody to Ribonuclease Inhibitor (RI)
PAB444Mu01 核糖核酸抑制因子(RI)多克隆抗体 Polyclonal Antibody to Ribonuclease Inhibitor (RI)
PAD163Hu01 核糖核酸酶Ⅲ(RNASEN)多克隆抗体 Polyclonal Antibody to Ribonuclease III, Nuclear (RNASEN)
PAA200Hu01 核糖核酸酶H(RNASEH)多克隆抗体 Polyclonal Antibody to Ribonuclease H (RNASEH)
PAQ867Hu01 核糖核酸酶A9(RNASE9)多克隆抗体 Polyclonal Antibody to Ribonuclease A9 (RNASE9)
PAD191Hu01 核糖核酸酶A8(RNASE8)多克隆抗体 Polyclonal Antibody to Ribonuclease A8 (RNASE8)
PAD193Hu01 核糖核酸酶A7(RNASE7)多克隆抗体 Polyclonal Antibody to Ribonuclease A7 (RNASE7)
PAD192Hu01 核糖核酸酶A6(RNASE6)多克隆抗体 Polyclonal Antibody to Ribonuclease A6 (RNASE6)
PAD190Hu01 核糖核酸酶A4(RNASE4)多克隆抗体 Polyclonal Antibody to Ribonuclease A4 (RNASE4)
PAB758Hu01 核糖核酸酶A3(RNASE3)多克隆抗体 Polyclonal Antibody to Ribonuclease A3 (RNASE3)
PAB758Mu01 核糖核酸酶A3(RNASE3)多克隆抗体 Polyclonal Antibody to Ribonuclease A3 (RNASE3)
PAB758Ra01 核糖核酸酶A3(RNASE3)多克隆抗体 Polyclonal Antibody to Ribonuclease A3 (RNASE3)
PAD169Hu01 核糖核酸酶A2(RNASE2)多克隆抗体 Polyclonal Antibody to Ribonuclease A2 (RNASE2)
PAT665Hu01 核糖核酸酶A13(RNASE13)多克隆抗体 Polyclonal Antibody to Ribonuclease A13 (RNASE13)
PAT642Hu01 核糖核酸酶A12(RNASE12)多克隆抗体 Polyclonal Antibody to Ribonuclease A12 (RNASE12)
PAT488Hu01 核糖核酸酶A11(RNASE11)多克隆抗体 Polyclonal Antibody to Ribonuclease A11 (RNASE11)
PAQ746Hu01 核糖核酸酶A10(RNASE10)多克隆抗体 Polyclonal Antibody to Ribonuclease A10 (RNASE10)
PAA297Hu01 核糖核酸酶A(RNASE1)多克隆抗体 Polyclonal Antibody to Ribonuclease A (RNASE1)
PAA297Bo01 核糖核酸酶A(RNASE1)多克隆抗体 Polyclonal Antibody to Ribonuclease A (RNASE1)
PAA297Mu01 核糖核酸酶A(RNASE1)多克隆抗体 Polyclonal Antibody to Ribonuclease A (RNASE1)
PAA297Ra01 核糖核酸酶A(RNASE1)多克隆抗体 Polyclonal Antibody to Ribonuclease A (RNASE1)
PAH234Hu01 核黄素激酶(RFK)多克隆抗体 Polyclonal Antibody to Riboflavin Kinase (RFK)
PAC777Hu01 视紫质(RHO)多克隆抗体 Polyclonal Antibody to Rhodopsin (RHO)
PAC777Mu01 视紫质(RHO)多克隆抗体 Polyclonal Antibody to Rhodopsin (RHO)
PAC777Mu02 视紫质(RHO)多克隆抗体 Polyclonal Antibody to Rhodopsin (RHO)
PAJ851Ra01 Rho鸟嘌呤核苷酸交换因子7(ARHGEF7)多克隆抗体 Polyclonal Antibody to Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7)
PAE330Hu01 Rho GDP解离抑制因子β(ARHGDIβ)多克隆抗体 Polyclonal Antibody to Rho GDP Dissociation Inhibitor Beta (ARHGDIb)
PAE329Hu01 Rho GDP解离抑制因子α(ARHGDIα)多克隆抗体 Polyclonal Antibody to Rho GDP Dissociation Inhibitor Alpha (ARHGDIa)
PAB852Hu01 含Rho关联卷曲螺旋蛋白激酶2(Rock2)多克隆抗体 Polyclonal Antibody to Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2)
PAB852Ra01 微管相关蛋白4抗体含Rho关联卷曲螺旋蛋白激酶2(Rock2)多克隆抗体 Polyclonal Antibody to Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2)
PAE705Mu01 Rho关联含卷曲螺旋结合蛋白激酶1(Rock1)多克隆抗体 Polyclonal Antibody to Rho Associated Coiled Coil Containing Protein Kinase 1 (Rock1)
PAP729Hu01 Rh血型D抗原(RHD)多克隆抗体 Polyclonal Antibody to Rh Blood Group, D Antigen (RHD)
PAC022Ge01 反三碘甲状腺原氨酸(rT3)多克隆抗体 Polyclonal Antibody to Reverse Triiodothyronine (rT3)