?纤微丝相关糖蛋白2抗体
『别名』MAGP 2; MAGP2; MAGP-2; MFAP 5; MFAP5; MFAP-5; Microfibril associated glycoprotein 2; microfibrillar associated protein 5; Microfibrillar associated protein 5 precursor; MP25.MFAP5_HUMAN
『浓度』?纤微丝相关糖蛋白2抗体1mg/1ml
『规格』0.1ml/100μg 0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
?纤微丝相关糖蛋白2抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
?纤微丝相关糖蛋白2抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
PAB588Ra01 信号转导衔接分子1(STAM1)多克隆抗体 Polyclonal Antibody to Signal Transducing Adaptor Molecule 1 (STAM1)
PAB737Hu01 信号传导转录激活因子6(STAT6)多克隆抗体 Polyclonal Antibody to Signal Transducer And Activator Of Transcription 6 (STAT6)
PAB737Ra01 信号传导转录激活因子6(STAT6)多克隆抗体 Polyclonal Antibody to Signal Transducer And Activator Of Transcription 6 (STAT6)
PAB737Mu01 信号传导转录激活因子6(STAT6)多克隆抗体 Polyclonal Antibody to Signal Transducer And Activator Of Transcription 6 (STAT6)
PAB727Mi01 信号传导转录激活因子5B(STAT5B)多克隆抗体 Polyclonal Antibody to Signal Transducer And Activator Of Transcription 5B (STAT5B)
PAB727Hu01 信号传导转录激活因子5B(STAT5B)多克隆抗体 Polyclonal Antibody to Signal Transducer And Activator Of Transcription 5B (STAT5B)
PAB738Hu02 信号传导转录激活因子5A(STAT5A)多克隆抗体 Polyclonal Antibody to Signal Transducer And Activator Of Transcription 5A (STAT5A)
PAB738Hu01 信号传导转录激活因子5A(STAT5A)多克隆抗体 Polyclonal Antibody to Signal Transducer And Activator Of Transcription 5A (STAT5A)
PAB739Hu01 信号传导转录激活因子4(STAT4)多克隆抗体 Polyclonal Antibody to Signal Transducer And Activator Of Transcription 4 (STAT4)
PAB743Bo01 信号传导转录激活因子3(STAT3)多克隆抗体 Polyclonal Antibody to Signal Transducer And Activator Of Transcription 3 (STAT3)
PAB743Ra02 信号传导转录激活因子3(STAT3)多克隆抗体 Polyclonal Antibody to Signal Transducer And Activator Of Transcription 3 (STAT3)
PAB743Ra01 信号传导转录激活因子3(STAT3)多克隆抗体 Polyclonal Antibody to Signal Transducer And Activator Of Transcription 3 (STAT3)
PAB743Mu02 信号传导转录激活因子3(STAT3)多克隆抗体 Polyclonal Antibody to Signal Transducer And Activator Of Transcription 3 (STAT3)
PAB743Mu01 信号传导转录激活因子3(STAT3)多克隆抗体 Polyclonal Antibody to Signal Transducer And Activator Of Transcription 3 (STAT3)
PAB743Hu01 信号传导转录激活因子3(STAT3)多克隆抗体 Polyclonal Antibody to Signal Transducer And Activator Of Transcription 3 (STAT3)
PAB796Hu01 信号传导转录激活因子2(STAT2)多克隆抗体 Polyclonal Antibody to Signal Transducer And Activator Of Transcription 2 (STAT2)
PAB796Mu01 信号传导转录激活因子2(STAT2)多克隆抗体 Polyclonal Antibody to Signal Transducer And Activator Of Transcription 2 (STAT2)
PAB796Ra01 信号传导转录激活因子2(STAT2)多克隆抗体 Polyclonal Antibody to Signal Transducer And Activator Of Transcription 2 (STAT2)
PAB740Hu01 信号传导转录激活因子1(STAT1)多克隆抗体 Polyclonal Antibody to Signal Transducer And Activator Of Transcription 1 (STAT1)
PAB740Mu01 信号传导转录激活因子1(STAT1)多克隆抗体 Polyclonal Antibody to Signal Transducer And Activator Of Transcription 1 (STAT1)
PAA947Hu01 信号调节蛋白γ(SIRPγ)多克隆抗体 Polyclonal Antibody to Signal Regulatory Protein Gamma (SIRPg)
PAA949Hu01 信号调节蛋白β1(SIRPβ1)多克隆抗体 Polyclonal Antibody to Signal Regulatory Protein Beta 1 (SIRPb1)
PAA949Mu01 信号调节蛋白β1(SIRPβ1)多克隆抗体 Polyclonal Antibody to Signal Regulatory Protein Beta 1 (SIRPb1)
PAA953Hu01 信号调节蛋白α(SIRPα)多克隆抗体 Polyclonal Antibody to Signal Regulatory Protein Alpha (SIRPa)
PAA953Ra01 信号调节蛋白α(SIRPα)多克隆抗体 Polyclonal Antibody to Signal Regulatory Protein Alpha (SIRPa)
PAQ389Hu01 CUB域EGF样信号肽3(SCUBE3)多克隆抗体 Polyclonal Antibody to Signal Peptide, CUB Domain, EGF Like 3 (SCUBE3)
PAC843Mu01 Sideroflexin 1蛋白(SFXN1)多克隆抗体 Polyclonal Antibody to Sideroflexin 1 (SFXN1)
PAC843Mu02 Sideroflexin 1蛋白(SFXN1)多克隆抗体 Polyclonal Antibody to Sideroflexin 1 (SFXN1)
PAE279Ra01 唾液酸酶2(SIAL2)多克隆抗体 Polyclonal Antibody to Sialidase 2, Cytosolic (SIAL2)
PAD922Hu01 唾液酸结合Ig样凝集素9(SIGLEC9)多克隆抗体 Polyclonal Antibody to Sialic Acid Binding Ig Like Lectin 9 (SIGLEC9)
PAB790Hu01 唾液酸结合Ig样凝集素8(SIGLEC8)多克隆抗体 Polyclonal Antibody to Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8)
PAB789Hu01 唾液酸结合Ig样凝集素7(SIGLEC7)多克隆抗体 Polyclonal Antibody to Sialic Acid Binding Ig Like Lectin 7 (SIGLEC7)
PAB789Hu02 唾液酸结合Ig样凝集素7(SIGLEC7)多克隆抗体 Polyclonal Antibody to Sialic Acid Binding Ig Like Lectin 7 (SIGLEC7)
PAB789Hu03 唾液酸结合Ig样凝集素7(SIGLEC7)多克隆抗体 Polyclonal Antibody to Sialic Acid Binding Ig Like Lectin 7 (SIGLEC7)
PAA961Hu01 唾液酸结合Ig样凝集素5(SIGLEC5)多克隆抗体 Polyclonal Antibody to Sialic Acid Binding Ig Like Lectin 5 (SIGLEC5)
PAA961Hu02 唾液酸结合Ig样凝集素5(SIGLEC5)多克隆抗体 Polyclonal Antibody to Sialic Acid Binding Ig Like Lectin 5 (SIGLEC5)
PAA961Hu03 唾液酸结合Ig样凝集素5(SIGLEC5)多克隆抗体 Polyclonal Antibody to Sialic Acid Binding Ig Like Lectin 5 (SIGLEC5)
PAA020Mu01 唾液酸结合Ig样凝集素3(SIGLEC3)多克隆抗体 Polyclonal Antibody to Sialic Acid Binding Ig Like Lectin 3 (SIGLEC3)
PAA020Mu02 唾液酸结合Ig样凝集素3(SIGLEC3)多克隆抗体 Polyclonal Antibody to Sialic Acid Binding Ig Like Lectin 3 (SIGLEC3)
PAB144Hu01 唾液酸结合Ig样凝集素2(SIGLEC2)多克隆抗体 Polyclonal Antibody to Sialic Acid Binding Ig Like Lectin 2 (SIGLEC2)
PAS344Hu01 唾液酸结合Ig样凝集素14(SIGLEC14)多克隆抗体 Polyclonal Antibody to Sialic Acid Binding Ig Like Lectin 14 (SIGLEC14)
PAB245Mu01 唾液酸结合Ig样凝集素12(SIGLEC12)多克隆抗体 Polyclonal Antibody to Sialic Acid Binding Ig Like Lectin 12 (SIGLEC12)
PAB245Hu01 唾液酸结合Ig样凝集素12(SIGLEC12)多克隆抗体 Polyclonal Antibody to Sialic Acid Binding Ig Like Lectin 12 (SIGLEC12)
PAB245Hu02 唾液酸结合Ig样凝集素12(SIGLEC12)多克隆抗体 Polyclonal Antibody to Sialic Acid Binding Ig Like Lectin 12 (SIGLEC12)
PAD921Hu01 唾液酸结合Ig样凝集素10(SIGLEC10)多克隆抗体 Polyclonal Antibody to Sialic Acid Binding Ig Like Lectin 10 (SIGLEC10)
PAA966Hu01 唾液酸结合Ig样凝集素1(SIGLEC1)多克隆抗体 Polyclonal Antibody to Sialic Acid Binding Ig Like Lectin 1 (SIGLEC1)
PAA966Hu02 唾液酸结合Ig样凝集素1(SIGLEC1)多克隆抗体 Polyclonal Antibody to Sialic Acid Binding Ig Like Lectin 1 (SIGLEC1)
PAH168Hu01 唾液酸乙酰酯酶(SIAE)多克隆抗体 Polyclonal Antibody to Sialic Acid Acetylesterase (SIAE)
PAS099Ge01 唾液酸(SA)多克隆抗体 Polyclonal Antibody to Sialic Acid (SA)
PAT373Hu01 短腭肺鼻腔上皮癌关联蛋白3(SPLUNC3)多克隆抗体 Polyclonal Antibody to Short Palate, Lung And Nasal Epithelium Carcinoma Associated Protein 3 (SPLUNC3)
PAT372Hu01 短腭肺鼻腔上皮癌关联蛋白2(SPLUNC2)多克隆抗体 Polyclonal Antibody to Short Palate, Lung And Nasal Epithelium Carcinoma Associated Protein 2 (SPLUNC2)
PAU613Hu01 Shisa同源物4(SHISA4)多克隆抗体 Polyclonal Antibody to Shisa Homolog 4 (SHISA4)
PAU613Mu01 Shisa同源物4(SHISA4)多克隆抗体 Polyclonal Antibody to Shisa Homolog 4 (SHISA4)
PAE670Hu01 SHC-转化蛋白3(SHC3)多克隆抗体 Polyclonal Antibody to SHC-Transforming Protein 3 (SHC3)
PAB751Mu01 SHC-转化蛋白2(SHC2)多克隆抗体 Polyclonal Antibody to SHC-Transforming Protein 2 (SHC2)
PAE671Hu01 SHC-转化蛋白1?纤微丝相关糖蛋白2抗体(SHC1)多克隆抗体 Polyclonal Antibody to SHC-Transforming Protein 1 (SHC1)
PAH176Hu01 SH3域结合蛋白2(SH3BP2)多克隆抗体 Polyclonal Antibody to SH3 Domain Binding Protein 2 (SH3BP2)
PAP692Hu01 SH3域结合谷氨酸丰富蛋白样蛋白(SH3BGRL)多克隆抗体 Polyclonal Antibody to SH3 Domain Binding Glutamic Acid Rich Protein Like Protein (SH3BGRL)
PAP694Mu01 含SH2域蛋白1A(SH2D1A)多克隆抗体 Polyclonal Antibody to SH2 Domain Containing Protein 1A (SH2D1A)